Mercurial > repos > nick > dunovo
diff dunovo.xml @ 0:f875256c722e draft
planemo upload for repository https://github.com/galaxyproject/dunovo commit b'd00f828e5768c5fac3e382b9d12f34bbdf9019e9\n'-dirty
author | nick |
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date | Sat, 18 Feb 2017 05:58:44 -0500 |
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children | ea832c221ec9 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/dunovo.xml Sat Feb 18 05:58:44 2017 -0500 @@ -0,0 +1,75 @@ +<?xml version="1.0"?> +<tool id="dunovo" name="Du Novo: Make consensus reads" version="0.7"> + <description>from duplex sequencing alignments</description> + <requirements> + <requirement type="package" version="0.7">dunovo</requirement> + <!-- TODO: require Python 2.7 --> + </requirements> + <command detect_errors="exit_code">dunovo.sh -r $min_reads -q $qual_thres -F $qual_format '$input' '$dcs1' '$dcs2' + #if $keep_sscs: + '$sscs1' '$sscs2' + #end if + </command> + <inputs> + <param name="input" type="data" format="tabular" label="Aligned input reads" /> + <param name="min_reads" type="integer" value="3" min="1" label="Minimum reads per family" help="Single-strand families with fewer than this many reads will be skipped."/> + <param name="qual_thres" type="integer" value="25" min="1" label="Minimum base quality" help="Bases with a PHRED score less than this will not be counted in the consensus making."/> + <param name="qual_format" type="select" label="FASTQ format" help="Solexa should also work for Illumina 1.3+ and 1.5+, and Sanger should work for Illumina 1.8+"> + <option value="sanger" selected="true">Sanger (PHRED 0 = "!")</option> + <option value="solexa">Solexa (PHRED 0 = "@")</option> + </param> + <param name="keep_sscs" type="boolean" truevalue="true" falsevalue="" label="Output single-strand consensus sequences as well" /> + </inputs> + <outputs> + <data name="dcs1" format="fasta" label="$tool.name on $on_string (mate 1)"/> + <data name="dcs2" format="fasta" label="$tool.name on $on_string (mate 2)"/> + <data name="sscs1" format="fasta" label="$tool.name on $on_string (SSCS mate 1)"> + <filter>keep_sscs</filter> + </data> + <data name="sscs2" format="fasta" label="$tool.name on $on_string (SSCS mate 2)"> + <filter>keep_sscs</filter> + </data> + </outputs> + <tests> + <test> + <param name="input" value="families.msa.tsv"/> + <output name="dcs1" file="families.cons_1.fa"/> + <output name="dcs2" file="families.cons_2.fa"/> + </test> + </tests> + <citations> + <citation type="bibtex">@article{Stoler2016, + author = {Stoler, Nicholas and Arbeithuber, Barbara and Guiblet, Wilfried and Makova, Kateryna D and Nekrutenko, Anton}, + doi = {10.1186/s13059-016-1039-4}, + issn = {1474-760X}, + journal = {Genome biology}, + number = {1}, + pages = {180}, + pmid = {27566673}, + publisher = {Genome Biology}, + title = {{Streamlined analysis of duplex sequencing data with Du Novo.}}, + url = {http://www.ncbi.nlm.nih.gov/pubmed/27566673}, + volume = {17}, + year = {2016} + }</citation> + </citations> + <help> + +**What it does** + +This is for processing duplex sequencing data. It creates single-strand and duplex consensus reads from aligned read families. + +----- + +**Input** + +This expects the output format of the "Align families" tool. + +----- + +**Output** + +This will output final, duplex consensus reads in two FASTA files (first and second reads in the pairs). Optionally, you can save the single-strand reads too, in a separate FASTA file. + + </help> +</tool>