Mercurial > repos > nikhil-joshi > deseq_and_sam2counts
diff deseq/deseq.xml @ 3:a49aff09553e draft default tip
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author | nikhil-joshi |
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date | Wed, 09 Jan 2013 18:39:12 -0500 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/deseq/deseq.xml Wed Jan 09 18:39:12 2013 -0500 @@ -0,0 +1,31 @@ +<tool id="deseq" name="DE Seq"> + <description>Run Differential Expression analysis from SAM To Count data</description> + + <command interpreter="python"> + stderr_wrapper.py Rscript ${GALAXY_DATA_INDEX_DIR}/deseq.R $counts $column_types $comparison $top_table $diagnostic_html "$diagnostic_html.files_path" "$counts.name" + </command> + + <inputs> + <param format="tabular" name="counts" type="data" optional="false" label="Counts file (from sam2counts)" help="Must have same number of samples as the column types field. E.g. if the column types field is 'kidney,kidney,liver,liver', then there must be 4 sample columns in the counts file."/> + + <param name="column_types" type="text" size="60" optional="false" label="Column Types in counts file" help="A comma separated list (no spaces) of the types of the data columns using the same name for replicates. E.g. kidney,kidney,kidney,liver,liver,liver"> + <validator type="empty_field"/> + <validator type="regex" message="Must be a comma-separated list with no spaces">^(\w+,)+\w+$</validator> + </param> + + <param name="comparison" type="text" size="30" optional="false" label="Comparison type" help="A comma separated tuple (no spaces) of the comparison you want to do. Must use the names from the Column Types list. E.g. comparing kidney to liver: kidney,liver. Comparing liver to kidney: liver,kidney"> + <validator type="empty_field"/> + <validator type="regex" message="Must be a comma-separated tuple with no spaces">^\w+,\w+$</validator> + </param> + </inputs> + + <outputs> + <data format="tabular" name="top_table" label="Top Table from ${tool.name} on ${on_string}"/> + <data format="html" name="diagnostic_html" label="Diagnostic Plots for ${tool.name} on ${on_string}"/> + </outputs> + + <help> + NOTE: This DEseq Galaxy tool can only be run on counts files that are created from SAM files that have been aligned to a transcriptome. + </help> + +</tool>