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author | nikhil-joshi |
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date | Mon, 19 Sep 2011 19:04:41 -0400 |
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<tool id="mapper" name="Mapping to reference"> <description>Mapping using a bowtie index</description> <requirements> <requirement type="perl-module">mapper_wrapper.pl</requirement> </requirements> <command interpreter="perl"> ./mapper_wrapper.pl $bowtie_index_name $output_arf $bowtie_index_name.extra_files_path $reads #if $reads.extension.startswith("fastq"): -e #end if #if $reads.extension.startswith("fasta"): -c #end if #if str($convert_rna) == "convert_rna_true": -i #end if #if str($remove_non_atcgun) == "remove_non_atcgun_true": -j #end if #if str($adapter_seq) != "": -k $adapter_seq #end if #if str($discard_short_reads) != "": -l $discard_short_reads #end if #if str($collapse_reads) == "Collapsed Reads Fasta": -m #end if #if str($map_mismatch) == "map_mismatch_false": -q #end if #if str($map_threshold) != "": -r $map_threshold #end if -h -s $output_fasta -n </command> <inputs> <param format="fastq, fasta" name="reads" type="data" optional="false" label="Reads" help="Reads in fastq or fasta format"/> <param format="bowtie_html_index" name="bowtie_index_name" type="data" optional="false" label="Bowtie indexed reference" help="Select the bowtie-build run, NOT the fasta reference file you indexed."/> <param name="convert_rna" type="boolean" truevalue="convert_rna_true" falsevalue="convert_rna_false" checked="false" label="Convert RNA to DNA alphabet (to map against genome)"/> <param name="remove_non_atcgun" type="boolean" truevalue="remove_non_atcgun_true" falsevalue="remove_non_atcgun_false" checked="false" label="Remove reads with non-standard nucleotides" help="Remove all entries that have a sequence that contains letters other than a,c,g,t,u,n,A,C,G,T,U,N"/> <param name="adapter_seq" value="" type="text" optional="true" label="Clip 3' Adapter Sequence (optional)" help="Adapter Sequence can only contain a,c,g,t,u,n,A,C,G,T,U,N"> <validator type="regex" message="Adapter can ONLY contain a,c,g,t,u,n,A,C,G,T,U,N">^[ACGTUacgtu]+$</validator> </param> <param name="discard_short_reads" value="17" type="integer" optional="false" label="Discard reads shorter than this length (0 for keeping all reads)" help="Note that miRDeep2 requires no reads under 17 in length"> <validator type="in_range" min="0" message="Minimum value is 0"/> </param> <param name="collapse_reads" type="boolean" truevalue="Collapsed Reads Fasta" falsevalue="Fasta" checked="true" label="Collapse identical reads into one read with count information in sequence header (default)"/> <param name="map_mismatch" type="boolean" truevalue="map_mismatch_true" falsevalue="map_mismatch_false" checked="false" label="Map with one mismatch in the seed (mapping takes longer)"/> <param name="map_threshold" value="5" type="integer" optional="false" label="A read is allowed to map up to this number of positions in the genome"> <validator type="in_range" min="1" message="Minimum value is 1"/> </param> </inputs> <outputs> <data format="fasta" name="output_fasta" label="$collapse_reads of ${tool.name} on ${on_string}"/> <data format="arf" name="output_arf" label="Mapping output of ${tool.name} on ${on_string} in ARF format"/> </outputs> </tool>