Mercurial > repos > nilesh > rseqc
comparison tin.xml @ 57:f437057e46f1 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 7d7cd4324af66710b89801a1a1c79fb8abf0d146
author | iuc |
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date | Thu, 27 Sep 2018 14:23:52 -0400 |
parents | 5873cd7afb67 |
children | 1a052c827e88 |
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56:daae0a118c36 | 57:f437057e46f1 |
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87 complex”. Therefore, RIN is practically not very useful in downstream | 87 complex”. Therefore, RIN is practically not very useful in downstream |
88 analysis such as adjusting the gene expression count. | 88 analysis such as adjusting the gene expression count. |
89 | 89 |
90 * RIN has very limited sensitivity to measure substantially degraded RNA | 90 * RIN has very limited sensitivity to measure substantially degraded RNA |
91 samples such as preserved clinical tissues. (ref: | 91 samples such as preserved clinical tissues. (ref: |
92 http://www.illumina.com/documents/products/technotes/technote-truseq-rna-access.pdf). | 92 https://www.illumina.com/content/dam/illumina-marketing/documents/products/technotes/technote-truseq-rna-access.pdf). |
93 | 93 |
94 To overcome these limitations, we developed TIN, an algorithm that is able | 94 To overcome these limitations, we developed TIN, an algorithm that is able |
95 to measure RNA integrity at transcript level. TIN calculates a score (0 <= | 95 to measure RNA integrity at transcript level. TIN calculates a score (0 <= |
96 TIN <= 100) for each expressed transcript, however, the medTIN (i.e. | 96 TIN <= 100) for each expressed transcript, however, the medTIN (i.e. |
97 meidan TIN score across all the transcripts) can also be used to measure | 97 meidan TIN score across all the transcripts) can also be used to measure |