Mercurial > repos > nilesh > rseqc
diff insertion_profile.xml @ 51:09846d5169fa draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
author | iuc |
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date | Tue, 14 Mar 2017 10:23:21 -0400 |
parents | |
children | dbedfc5f5a3c |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/insertion_profile.xml Tue Mar 14 10:23:21 2017 -0400 @@ -0,0 +1,88 @@ +<tool id="rseqc_insertion_profile" name="Insertion Profile" version="@WRAPPER_VERSION@"> + <description> + calculates the distribution of inserted nucleotides across reads + </description> + + <macros> + <import>rseqc_macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <expand macro="stdio" /> + + <version_command><![CDATA[insertion_profile.py --version]]></version_command> + + <command><![CDATA[ + insertion_profile.py -i '${input}' -o output -q ${mapq} -s "${layout}" + ]]> + </command> + + <inputs> + <expand macro="bam_param" /> + <expand macro="mapq_param" /> + <expand macro="layout_param" /> + <expand macro="rscript_output_param" /> + </inputs> + + <outputs> + <expand macro="pdf_output_data" filename="output.insertion_profile.pdf" /> + <expand macro="xls_output_data" filename="output.insertion_profile.xls" /> + <expand macro="rscript_output_data" filename="output.insertion_profile.r" /> + </outputs> + + <tests> + <test> + <param name="input" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam" /> + <param name="rscript_output" value="true" /> + <output name="outputpdf" file="output.insertion_profile.pdf" compare="sim_size" /> + <output name="outputxls" file="output.insertion_profile.xls" /> + <output name="outputr" file="output.insertion_profile.r" /> + </test> + </tests> + + <help><![CDATA[ +insertion_profile.py +++++++++++++++++++++ + +Calculate the distributions of inserted nucleotides across reads. Note that to +use this funciton, CIGAR strings within SAM/BAM file should have āIā operation. + +Inputs +++++++ + +Input BAM/SAM file + Alignment file in BAM/SAM format. + +Minimum mapping quality + Minimum mapping quality for an alignment to be considered as "uniquely + mapped". default=30 + +Sequencing layout + Denotes whether the sequecing was single-end (SE) or paired-end (PE). + +Sample Output +++++++++++++++ + +Read-1 insertion profile: + +.. image:: $PATH_TO_IMAGES/out.insertion_profile.R1.png + :height: 600 px + :width: 600 px + :scale: 80 % + +Read-2 insertion profile: + +.. image:: $PATH_TO_IMAGES/out.insertion_profile.R2.png + :height: 600 px + :width: 600 px + :scale: 80 % + +@ABOUT@ + +]]> + </help> + + <expand macro="citations" /> + +</tool>