Mercurial > repos > nilesh > rseqc
diff read_hexamer.xml @ 51:09846d5169fa draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
author | iuc |
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date | Tue, 14 Mar 2017 10:23:21 -0400 |
parents | |
children | dbedfc5f5a3c |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/read_hexamer.xml Tue Mar 14 10:23:21 2017 -0400 @@ -0,0 +1,137 @@ +<tool id="rseqc_read_hexamer" name="Hexamer frequency" version="@WRAPPER_VERSION@"> + <description> + calculates hexamer (6mer) frequency for reads, genomes, and mRNA sequences + </description> + + <macros> + <import>rseqc_macros.xml</import> + </macros> + + <expand macro="requirements" /> + + <expand macro="stdio" /> + + <version_command><![CDATA[read_hexamer.py --version]]></version_command> + + <command><![CDATA[ + #import re + #set $input_list = [] + #for $i, $input in enumerate($inputs): + + #set $safename = re.sub('[^\w\-_]', '_', $input.element_identifier) + #if $safename in $input_list: + #set $safename = str($safename) + "." + str($i) + #end if + $input_list.append($safename) + + #if $input.is_of_type("fastq.gz", "fastqsanger.gz"): + gunzip -c '${input}' > "${safename}" && + #else: + ln -sf '${input}' "${safename}" && + #end if + #end for + read_hexamer.py -i '${ ','.join( [ $name for $name in $input_list ] ) }' + #if $refgenome: + -r '${refgenome}' + #end if + #if $refgene: + -g '${refgene}' + #end if + > '${output}' + ]]> + </command> + + <inputs> + <param name="inputs" type="data" label="Read sequences in fasta or fastq format" format="fasta,fastq,fastqsanger,fastq.gz,fastqsanger.gz" help="(--input)" multiple="true" /> + <param name="refgenome" type="data" label="Reference genome seqeunce (fasta)" format="fasta" optional="true" help="(--refgenome)" /> + <param name="refgene" type="data" label="Reference mRNA sequence (fasta)" format="fasta" optional="true" help="(--refgene)" /> + </inputs> + + <outputs> + <data name="output" format="tabular" label="${tool.name} on ${on_string}" /> + </outputs> + + <tests> + <test> + <param name="inputs" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq"/> + <output name="output"> + <assert_contents> + <has_line line="Hexamer	pairend_strandspecific_51mer_hg19_chr1_1-100000_R1_fastq" /> + <has_line line="AAAAAA	0.00217391304348" /> + </assert_contents> + </output> + </test> + <test> + <param name="inputs" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq.gz" ftype="fastqsanger.gz"/> + <output name="output"> + <assert_contents> + <has_line line="Hexamer	pairend_strandspecific_51mer_hg19_chr1_1-100000_R1_fastq_gz" /> + <has_line line="AAAAAA	0.00217391304348" /> + </assert_contents> + </output> + </test> + <test> + <param name="inputs" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq,pairend_strandspecific_51mer_hg19_chr1_1-100000.R2.fastq"/> + <output name="output"> + <assert_contents> + <has_line line="Hexamer	pairend_strandspecific_51mer_hg19_chr1_1-100000_R1_fastq	pairend_strandspecific_51mer_hg19_chr1_1-100000_R2_fastq" /> + <has_line line="AAAAAA	0.00217391304348	0.00534759358289" /> + </assert_contents> + </output> + </test> + <test> + <param name="inputs" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq,pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq"/> + <output name="output"> + <assert_contents> + <has_line line="Hexamer	pairend_strandspecific_51mer_hg19_chr1_1-100000_R1_fastq	pairend_strandspecific_51mer_hg19_chr1_1-100000_R1_fastq.1" /> + <has_line line="AAAAAA	0.00217391304348	0.00217391304348" /> + </assert_contents> + </output> + </test> + <!-- Unable to test with collections at the moment (requires type="data_collection" on the input) + <test> + <param name="inputs"> + <collection type="list"> + <element name="read_1" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq" /> + <element name="read_2" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R2.fastq" /> + </collection> + </param> + <output name="output" file="output.read_hexamer.2.txt" /> + </test> + --> + </tests> + + <help><![CDATA[ +read_hexamer.py ++++++++++++++++++++++ + +Calculate hexamer (6mer) frequency. If ‘-r’ was specified, hexamer frequency +is also calculated for the reference genome. If ‘-g’ was provided, hexamer +frequency is also calculated for the mRNA sequences. + +Inputs +++++++++++++++ + +Input reads file + Read sequences in fasta or fastq format. + +Reference Genome + Reference genome sequence in fasta format. + +Reference Gene + Reference mRNA sequences in fasta format. + + +Outputs +++++++++++++++ + +Tabular file of hexamer frequences in for each input. + +@ABOUT@ + +]]> + </help> + + <expand macro="citations" /> + +</tool>