diff read_GC.xml @ 49:6b33e31bda10 draft

Uploaded tar based on https://github.com/lparsons/galaxy_tools/tree/master/tools/rseqc 1a3c419bc0ded7c40cb2bc3e7c87bfb01ddfeba2
author lparsons
date Thu, 16 Jul 2015 17:43:43 -0400
parents eb339c5849bb
children 09846d5169fa
line wrap: on
line diff
--- a/read_GC.xml	Tue Apr 21 10:27:06 2015 -0400
+++ b/read_GC.xml	Thu Jul 16 17:43:43 2015 -0400
@@ -1,26 +1,48 @@
-<tool id="rseqc_read_GC" name="Read GC" version="2.4">
+<tool id="rseqc_read_GC" name="Read GC" version="2.4galaxy1">
     <description>determines GC% and read count</description>
+
+    <macros>
+        <import>rseqc_macros.xml</import>
+    </macros>
+
     <requirements>
-        <requirement type="package" version="3.0.3">R</requirement>
-        <requirement type="package" version="1.7.1">numpy</requirement>
-        <requirement type="package" version="2.4">rseqc</requirement>
+        <expand macro="requirement_package_r" />
+        <expand macro="requirement_package_numpy" />
+        <expand macro="requirement_package_rseqc" />
     </requirements>
-    <command>
-        read_GC.py -i $input -o output
+
+    <expand macro="stdio" />
+
+    <version_command><![CDATA[read_GC.py --version]]></version_command>
+
+    <command><![CDATA[
+        read_GC.py
+            --input-file $input
+            --out-prefix output
+            --mapq $mapq
+        ]]>
     </command>
-    <stdio>
-        <exit_code range="1:" level="fatal" description="An error occured during execution, see stderr and stdout for more information" />
-        <regex match="[Ee]rror" source="both" description="An error occured during execution, see stderr and stdout for more information" />
-    </stdio>
+
     <inputs>
-        <param name="input" type="data" format="bam,sam" label="input bam/sam file" />
+        <param name="input" type="data" format="bam,sam" label="input bam/sam file" help="(--input-file)"/>
+        <param name="mapq" type="integer" label="Minimum mapping quality (default=30)" help="Minimum phred scale mapping quality to consider a read 'uniquely mapped' (--mapq)" value="30" />
     </inputs>
+
     <outputs>
         <data format="xls" name="outputxls" from_work_dir="output.GC.xls" label="${tool.name} on ${on_string} (XLS)"/>
         <data format="txt" name="outputr" from_work_dir="output.GC_plot.r" label="${tool.name} on ${on_string} (R Script)" />
         <data format="pdf" name="outputpdf" from_work_dir="output.GC_plot.pdf" label="${tool.name} on ${on_string} (PDF)" />
     </outputs>
-    <help>
+
+    <tests>
+        <test>
+            <param name="input" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam"/>
+            <output name="outputxls" file="output.GC.xls"/>
+            <output name="outputr" file="output.GC_plot.r"/>
+        </test>
+    </tests>
+
+    <help><![CDATA[
 read_GC.py
 ++++++++++
 
@@ -36,16 +58,16 @@
 
 1. output.GC.xls: Two column, plain text file, first column is GC%, second column is read count
 2. output.GC_plot.r: R script to generate pdf file.
-3. output.GC_plot.pdf: graphical output generated from R script. 
+3. output.GC_plot.pdf: graphical output generated from R script.
 
-.. image:: http://rseqc.sourceforge.net/_images/read_gc.png 
+.. image:: http://rseqc.sourceforge.net/_images/read_gc.png
    :height: 600 px
    :width: 600 px
-   :scale: 80 %    
+   :scale: 80 %
 
 -----
 
-About RSeQC 
+About RSeQC
 +++++++++++
 
 The RSeQC_ package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data. "Basic modules" quickly inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while "RNA-seq specific modules" investigate sequencing saturation status of both splicing junction detection and expression estimation, mapped reads clipping profile, mapped reads distribution, coverage uniformity over gene body, reproducibility, strand specificity and splice junction annotation.
@@ -55,7 +77,9 @@
 .. image:: http://rseqc.sourceforge.net/_static/logo.png
 
 .. _RSeQC: http://rseqc.sourceforge.net/
-
+]]>
+    </help>
 
-    </help>
+    <expand macro="citations" />
+
 </tool>