Mercurial > repos > nilesh > rseqc
diff read_distribution.xml @ 49:6b33e31bda10 draft
Uploaded tar based on https://github.com/lparsons/galaxy_tools/tree/master/tools/rseqc 1a3c419bc0ded7c40cb2bc3e7c87bfb01ddfeba2
author | lparsons |
---|---|
date | Thu, 16 Jul 2015 17:43:43 -0400 |
parents | eb339c5849bb |
children | 09846d5169fa |
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--- a/read_distribution.xml Tue Apr 21 10:27:06 2015 -0400 +++ b/read_distribution.xml Thu Jul 16 17:43:43 2015 -0400 @@ -1,24 +1,42 @@ -<tool id="rseqc_read_distribution" name="Read Distribution" version="2.4"> +<tool id="rseqc_read_distribution" name="Read Distribution" version="2.4galaxy1"> <description>calculates how mapped reads were distributed over genome feature</description> + + <macros> + <import>rseqc_macros.xml</import> + </macros> + <requirements> - <requirement type="package" version="1.7.1">numpy</requirement> - <requirement type="package" version="2.4">rseqc</requirement> + <expand macro="requirement_package_numpy" /> + <expand macro="requirement_package_rseqc" /> </requirements> - <command> + + <expand macro="stdio" /> + + <version_command><![CDATA[read_distribution.py --version]]></version_command> + + <command><![CDATA[ read_distribution.py -i $input -r $refgene > $output + ]]> </command> - <stdio> - <exit_code range="1:" level="fatal" description="An error occured during execution, see stderr and stdout for more information" /> - <regex match="[Ee]rror" source="both" description="An error occured during execution, see stderr and stdout for more information" /> - </stdio> + <inputs> - <param name="input" type="data" format="bam,sam" label="input bam/sam file" /> - <param name="refgene" type="data" format="bed" label="reference gene model" /> + <param name="input" type="data" format="bam,sam" label="input bam/sam file" help="(--input-file)"/> + <param name="refgene" type="data" format="bed" label="reference gene model" help="(--refgene)"/> </inputs> + <outputs> <data format="txt" name="output" /> </outputs> - <help> + + <tests> + <test> + <param name="input" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam"/> + <param name="refgene" value="hg19_RefSeq_chr1_1-100000.bed"/> + <output name="output" file="output.read_distribution.txt"/> + </test> + </tests> + + <help><![CDATA[ read_distribution.py ++++++++++++++++++++ @@ -39,7 +57,7 @@ * hit to intergenic regions that beyond region starting from TSS upstream 10Kb to TES downstream 10Kb. * hit to regions covered by both 5'UTR and 3' UTR. This is possible when two head-to-tail transcripts are overlapped in UTR regions. -* hit to regions covered by both TSS upstream 10Kb and TES downstream 10Kb. +* hit to regions covered by both TSS upstream 10Kb and TES downstream 10Kb. Inputs @@ -57,23 +75,23 @@ Output: =============== ============ =========== =========== -Group Total_bases Tag_count Tags/Kb +Group Total_bases Tag_count Tags/Kb =============== ============ =========== =========== -CDS_Exons 33302033 20002271 600.63 -5'UTR_Exons 21717577 4408991 203.01 -3'UTR_Exons 15347845 3643326 237.38 -Introns 1132597354 6325392 5.58 -TSS_up_1kb 17957047 215331 11.99 -TSS_up_5kb 81621382 392296 4.81 -TSS_up_10kb 149730983 769231 5.14 -TES_down_1kb 18298543 266161 14.55 -TES_down_5kb 78900674 729997 9.25 -TES_down_10kb 140361190 896882 6.39 +CDS_Exons 33302033 20002271 600.63 +5'UTR_Exons 21717577 4408991 203.01 +3'UTR_Exons 15347845 3643326 237.38 +Introns 1132597354 6325392 5.58 +TSS_up_1kb 17957047 215331 11.99 +TSS_up_5kb 81621382 392296 4.81 +TSS_up_10kb 149730983 769231 5.14 +TES_down_1kb 18298543 266161 14.55 +TES_down_5kb 78900674 729997 9.25 +TES_down_10kb 140361190 896882 6.39 =============== ============ =========== =========== ----- -About RSeQC +About RSeQC +++++++++++ The RSeQC_ package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data. "Basic modules" quickly inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while "RNA-seq specific modules" investigate sequencing saturation status of both splicing junction detection and expression estimation, mapped reads clipping profile, mapped reads distribution, coverage uniformity over gene body, reproducibility, strand specificity and splice junction annotation. @@ -83,8 +101,9 @@ .. image:: http://rseqc.sourceforge.net/_static/logo.png .. _RSeQC: http://rseqc.sourceforge.net/ - - +]]> + </help> - </help> + <expand macro="citations" /> + </tool>