Mercurial > repos > nilesh > rseqc
view bam_stat.xml @ 53:080fd5739bb4 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 734a31ae8ba22f0d75bda696c4856ee3e274e1ba
author | iuc |
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date | Tue, 20 Mar 2018 05:37:48 -0400 |
parents | 09846d5169fa |
children | dbedfc5f5a3c |
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<tool id="rseqc_bam_stat" name="BAM/SAM Mapping Stats" version="@WRAPPER_VERSION@"> <description> reads mapping statistics for a provided BAM or SAM file. </description> <macros> <import>rseqc_macros.xml</import> </macros> <expand macro="requirements" /> <expand macro="stdio" /> <version_command><![CDATA[bam_stat.py --version]]></version_command> <command><![CDATA[ bam_stat.py -i '${input}' -q ${mapq} > '${output}' ]]> </command> <inputs> <expand macro="bam_param" /> <expand macro="mapq_param" /> </inputs> <outputs> <data format="txt" name="output" /> </outputs> <tests> <test> <param name="input" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.bam" /> <output name="output" file="output.bamstats.txt" /> </test> </tests> <help><![CDATA[ bam_stat.py +++++++++++ This program is used to calculate reads mapping statistics from provided BAM file. This script determines "uniquely mapped reads" from `mapping quality <http://genome.sph.umich.edu/wiki/Mapping_Quality_Scores>`_, which quality the probability that a read is misplaced (Do NOT confused with sequence quality, sequence quality measures the probability that a base-calling was wrong) . Inputs ++++++++++++++ Input BAM/SAM file Alignment file in BAM/SAM format. Minimum mapping quality Minimum mapping quality for an alignment to be called "uniquely mapped" (default=30) Output ++++++++++++++ - Total Reads (Total records) = {Multiple mapped reads} + {Uniquely mapped} - Uniquely mapped Reads = {read-1} + {read-2} (if paired end) - Uniquely mapped Reads = {Reads map to '+'} + {Reads map to '-'} - Uniquely mapped Reads = {Splice reads} + {Non-splice reads} @ABOUT@ ]]> </help> <expand macro="citations" /> </tool>