changeset 15:2f55e1d520cd

Uploaded
author nilesh
date Thu, 11 Jul 2013 12:23:19 -0400
parents 6f150036a010
children 366799f58c6b
files bam_stat.xml
diffstat 1 files changed, 49 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/bam_stat.xml	Thu Jul 11 12:23:19 2013 -0400
@@ -0,0 +1,49 @@
+<tool id="bam_stat" name="BAM/SAM Mapping Stats">
+	<description>
+		reads mapping statistics for a provided BAM or SAM file.
+	</description>
+	<requirements>
+		<requirement type="package" version="2.3.7">rseqc</requirement>
+	</requirements>s
+	<command interpreter="python">
+		bam_stat.py -i $input -q $mapqual > $output
+	</command>
+	<inputs>
+		<param name="input" type="data" label="Input .bam/.sam File" format="bam,sam" />
+		<param label="Minimum mapping quality (default=30" type="integer" value="30" name="mapqual" />
+	</inputs>
+	<outputs>
+		<data format="txt" name="output" />
+	</outputs>
+	<help>
+.. image:: https://code.google.com/p/rseqc/logo?cct=1336721062
+
+-----
+
+About RSeQC
++++++++++++
+
+The RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data. “Basic modules” quickly inspect sequence quality, nucleotide composition bias, PCR bias and GC bias, while “RNA-seq specific modules” investigate sequencing saturation status of both splicing junction detection and expression estimation, mapped reads clipping profile, mapped reads distribution, coverage uniformity over gene body, reproducibility, strand specificity and splice junction annotation.
+
+The RSeQC package is licensed under the GNU GPL v3 license.
+
+Inputs
+++++++++++++++
+
+Input BAM/SAM file
+	Alignment file in BAM/SAM format.
+
+Minimum mapping quality
+	Minimum mapping quality for an alignment to be called “uniquely mapped” (default=30)
+
+Output
+++++++++++++++
+
+- Total Reads (Total records) = {Multiple mapped reads} + {Uniquely mapped}
+- Uniquely mapped Reads = {read-1} + {read-2} (if paired end)
+- Uniquely mapped Reads = {Reads map to '+'} + {Reads map to '-'}
+- Uniquely mapped Reads = {Splice reads} + {Non-splice reads}
+
+
+	</help>
+</tool>
\ No newline at end of file