annotate tmap_wrapper_0.0.19/tmap_wrapper.xml @ 1:40ade4f81a30 default tip

Uploaded tool_data_table_conf.xml.sample
author nate
date Fri, 23 Sep 2011 14:53:08 -0400
parents 7cbfd271d207
children
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1 <tool id="tmap_wrapper" name="Map with TMAP" version="0.0.19">
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2 <description>for Ion Torrent</description>
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3 <parallelism method="basic"></parallelism>
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4 <command interpreter="python">
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5 tmap_wrapper.py
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6
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7 ## reference source
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8 --fileSource=$genomeSource.refGenomeSource
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9 #if $genomeSource.refGenomeSource == "history":
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10 ##build index on the fly
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11 --ref="${genomeSource.ownFile}"
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12 --dbkey=$dbkey
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13 #else:
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14 ##use precomputed indexes
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15 --ref="${ filter( lambda x: str( x[0] ) == str( $genomeSource.indices ), $__app__.tool_data_tables[ 'tmap_indexes' ].get_fields() )[0][-1] }"
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16 --do_not_build_index
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17 #end if
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18
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19 ## input fastq
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20 --fastq=$input
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21
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22 ## output file
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23 --output=$output
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24
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25 ## run parameters
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26 --params=$params.source_select
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27 #if $params.source_select != "pre_set":
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28 --mapall=$params.mapall.source_select
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29 #if $params.mapall.source_select == "true":
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30 --threads=$params.mapall.threads
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31 --matchScore=$params.mapall.matchScore
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32 --mismatchPenalty=$params.mapall.mismatchPenalty
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33 --gapOpenPenalty=$params.mapall.gapOpenPenalty
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34 --gapExtensPenalty=$params.mapall.gapExtensPenalty
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35 --flowPenalty=$params.mapall.flowPenalty
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36 --flowOrder=$params.mappll.flowOrder
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37 --bandWidth=$params.mapall.bandWidth
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38 --globalMap=$params.mapall.globalMap
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39 --duplicateWindow=$params.mapall.duplicateWindow
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40 --scoringThreshold=$params.mapall.scoringThreshold
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41 --queueSize=$params.mapall.queueSize
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42 --outputFilter=$params.mapall.outputFilter
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43 --rgTag=$params.mapall.rgTag.source_select
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44 #if $params.mapall.rgTag.source_select == "true":
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45 --rgTagID=$params.mapall.rgTag.rgTagID
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46 --rgTagCN=$params.mapall.rgTag.rgTagCN
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47 --rgTagDS=$params.mapall.rgTag.rgTagDS
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48 --rgTagDT=$params.mapall.rgTag.rgTagDT
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49 --rgTagLB=$params.mapall.rgTag.rgTagLB
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50 --rgTagPI=$params.mapall.rgTag.rgTagPI
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51 --rgTagPL=$params.mapall.rgTag.rgTagPL
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52 --rgTagPU=$params.mapall.rgTag.rgTagPU
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53 --rgTagSM=$params.mapall.rgTag.rgTagSM
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54 #end if
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55 --filterIndependently=$params.mapall.filterIndependently
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56 #end if
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57 --map1=$params.map1.source_select
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58 #if $params.map1.source_select == "true":
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59 --map1SeedLength=$params.map1.map1SeedLength
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60 --map1SeedMismatches=$params.map1.map1SeedMismatches
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61 --map1SecondarySeedLength=$params.map1.map1SecondarySeedLength
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62 --map1NumEdits=$params.map1.map1NumEdits
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63 --map1BaseError=$params.map1.map1BaseError
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64 --map1Mismatches=$params.map1.map1Mismatches
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65 --map1GapOpens=$params.map1.map1GapOpens
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66 --map1GapExtensions=$params.map1.map1GapExtensions
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67 --map1MaxCALsDeletion=$params.map1.map1MaxCALsDeletion
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68 --map1EndIndels=$params.map1.map1EndIndels
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69 --map1MaxOptimalCALs=$params.map1.map1MaxOptimalCALs
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70 --map1MaxNodes=$params.map1.map1MaxNodes
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71 #end if
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72 --map2=$params.map2.source_select
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73 #if $params.map2.source_select == "true":
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74 --map2Coefficient=$params.map2.map2Coefficient
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75 --map2SeedIntervalSize=$params.map2.map2SeedIntervalSize
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76 --map2ZBest=$params.map2.map2ZBest
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77 --map2ReverseTrigger=$params.map2.map2ReverseTrigger
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78 #end if
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79 --map3=$params.map3.source_select
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80 #if $params.map3.source_select == "true":
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81 --map3SeedLength=$params.map3.map3SeedLength
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82 --map3SeedMaxHits=$params.map3.map3SeedMaxHits
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83 --map3SeedWindow=$params.map3.map3SeedWindow
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84 --map3HPEnumeration=$params.map3.map3HPEnumeration
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85 #end if
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86 --MAP1=$params.MAP1.source_select
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87 #if $params.MAP1.source_select == "true":
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88 --MAP1SeedLength=$params.MAP1.MAP1SeedLength
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89 --MAP1SeedMismatches=$params.MAP1.MAP1SeedMismatches
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90 --MAP1SecondarySeedLength=$params.MAP1.MAP1SecondarySeedLength
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91 --MAP1NumEdits=$params.MAP1.MAP1NumEdits
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92 --MAP1BaseError=$params.MAP1.MAP1BaseError
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93 --MAP1Mismatches=$params.MAP1.MAP1Mismatches
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94 --MAP1GapOpens=$params.MAP1.MAP1GapOpens
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95 --MAP1GapExtensions=$params.MAP1.MAP1GapExtensions
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96 --MAP1MaxCALsDeletion=$params.MAP1.MAP1MaxCALsDeletion
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97 --MAP1EndIndels=$params.MAP1.MAP1EndIndels
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98 --MAP1MaxOptimalCALs=$params.MAP1.MAP1MaxOptimalCALs
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99 --MAP1MaxNodes=$params.MAP1.MAP1MaxNodes
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100 #end if
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101 --MAP2=$params.MAP2.source_select
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102 #if $params.MAP2.source_select == "true":
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103 --MAP2Coefficient=$params.MAP2.MAP2Coefficient
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104 --MAP2SeedIntervalSize=$params.MAP2.MAP2SeedIntervalSize
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105 --MAP2ZBest=$params.MAP2.MAP2ZBest
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106 --MAP2ReverseTrigger=$params.MAP2.MAP2ReverseTrigger
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107 #end if
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108 --MAP3=$params.MAP3.source_select
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109 #if $params.MAP3.source_select == "true":
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110 --MAP3SeedLength=$params.MAP3.MAP3SeedLength
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111 --MAP3SeedMaxHits=$params.MAP3.MAP3SeedMaxHits
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112 --MAP3SeedWindow=$params.MAP3.MAP3SeedWindow
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113 --MAP3HPEnumeration=$params.MAP3.MAP3HPEnumeration
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114 #end if
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115 #else:
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116 --threads="4"
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117 --map1="false"
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118 --map2="false"
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119 --map3="false"
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120 --MAP1="false"
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121 --MAP2="false"
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122 --MAP3="false"
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123 #end if
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124
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125 ## suppress output SAM header
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126 --suppressHeader=$suppressHeader
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127 </command>
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128 <requirements>
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129 <requirement type='package'>tmap</requirement>
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130 </requirements>
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131 <inputs>
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132 <conditional name="genomeSource">
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133 <param name="refGenomeSource" type="select" label="Will you select a reference genome from your history or use a built-in index?">
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134 <option value="indexed">Use a built-in index</option>
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135 <option value="history">Use one from the history</option>
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136 </param>
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137 <when value="indexed">
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138 <param name="indices" type="select" label="Select a reference genome">
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139 <options from_data_table="tmap_indexes">
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140 <filter type="sort_by" column="3" />
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141 <validator type="no_options" message="No indexes are available for the selected input dataset" />
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142 </options>
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143 </param>
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diff changeset
144 </when>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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145 <when value="history">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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146 <param name="ownFile" type="data" format="fasta" metadata_name="dbkey" label="Select a reference from history" />
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147 </when>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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148 </conditional>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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149 <param name="input" type="data" format="fastqsanger" label="FASTQ file" help="Must have Sanger-scaled quality values with ASCII offset 33" />
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150 <conditional name="params">
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151 <param name="source_select" type="select" label="TMAP settings to use" help="For most mapping needs use Commonly Used settings. If you want full control use Full Parameter List">
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152 <option value="pre_set">Commonly Used</option>
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153 <option value="full">Full Parameter List</option>
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154 </param>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
155 <when value="pre_set" />
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156 <when value="full">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
157 <conditional name="mapall">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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158 <param name="source_select" type="select" label="Global options to use">
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159 <option value="false" selected="true">Commonly Used</option>
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160 <option value="true">Full Parameter List</option>
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161 </param>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
162 <when value="false" />
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163 <when value="true">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
164 <param name="threads" type="integer" value="4" label="The number of threads (-n)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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165 <param name="matchScore" type="integer" value="3" label="Match score (-A)" />
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166 <param name="mismatchPenalty" type="integer" value="5" label="Mismatch penalty (-M)" />
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diff changeset
167 <param name="gapOpenPenalty" type="integer" value="2" label="Gap open penalty (-O)" />
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168 <param name="gapExtensPenalty" type="integer" value="2" label="Gap extension penalty (-E)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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169 <param name="flowPenalty" type="integer" value="2" label="The score penalty (-X)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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170 <param name="flowOrder" type="text" value="" label="The flow order ([ACGT]{4+} or sff" />
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171 <param name="bandWidth" type="integer" value="50" label="The band width (-w)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
172 <param name="globalMap" type="integer" value="2" label="The soft clipping type (-g)" help="The soft-clipping type (0 - allow on the right and left, 1 - allow on the left, 2 - allow on the right, 3 - do not allow soft-clipping)" />
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diff changeset
173 <param name="duplicateWindow" type="integer" value="128" label="The duplicate window (-W)" help="Remove duplicate alignments from different algorithms within this bp window (-1 to disable)"/>
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174 <param name="scoringThreshold" type="integer" value="1" label="The scoring threshold (-T)" help="The score threshold divided by the match score" />
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diff changeset
175 <param name="queueSize" type="integer" value="262144" label="The queue size (-q)" help="The queue size for the reads" />
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diff changeset
176 <param name="outputFilter" type="integer" value="1" label="The output filter (-a)" help="The output filter (0 - unique best hits, 1 - random best hit, 2 - all best htis, 3 - all alignments)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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177 <conditional name="rgTag">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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178 <param name="source_select" type="select" label="Include Custom Read Group Tags">
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179 <option value="false" selected="true">Default</option>
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180 <option value="true">Custom</option>
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181 </param>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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182 <when value="false" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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183 <when value="true">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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184 <param name="rgTagID" type="text" value="" label="The Read Group ID (-R)" help="The RG ID used in the RG record in the SAM header" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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185 <param name="rgTagCN" type="text" value="" label="The Read Group CN (-R)" help="The RG CN used in the RG record in the SAM header" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
186 <param name="rgTagDS" type="text" value="" label="The Read Group DS (-R)" help="The RG DS used in the RG record in the SAM header" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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187 <param name="rgTagDT" type="text" value="" label="The Read Group DT (-R)" help="The RG DT used in the RG record in the SAM header" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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188 <param name="rgTagLB" type="text" value="" label="The Read Group LB (-R)" help="The RG LB used in the RG record in the SAM header" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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189 <param name="rgTagPI" type="text" value="" label="The Read Group PI (-R)" help="The RG PI used in the RG record in the SAM header" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
190 <param name="rgTagPL" type="text" value="" label="The Read Group PL (-R)" help="The RG PL used in the RG record in the SAM header" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
191 <param name="rgTagPU" type="text" value="" label="The Read Group PU (-R)" help="The RG PU used in the RG record in the SAM header" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
192 <param name="rgTagSM" type="text" value="" label="The Read Group SM (-R)" help="The RG SM used in the RG record in the SAM header" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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193 </when>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
194 </conditional>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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195 <param name="filterIndependently" type="boolean" truevalue="true" falsevalue="false" checked="no" label="Filter algorithm independently (-I)" help="apply the output filter for each algorithm separately" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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196 </when>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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197 </conditional>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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198 <conditional name="map1">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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199 <param name="source_select" type="select" label="Map1 Stage One" help="Turn on mapping algorithm #1 in the first stage">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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200 <option value="true">Turn On</option>
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201 <option value="false" selected="true">Turn Off</option>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
202 </param>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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203 <when value="true">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
204 <param name="map1SeedLength" type="integer" value="32" label="The k-mer length to seed CALs (-1 to disable) (-l)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
205 <param name="map1SeedMismatches" type="integer" value="2" label="The maximum number of mismatches in the seed (-s)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
206 <param name="map1SecondarySeedLength" type="integer" value="64" label="The secondary seed length (-1 to disable) (-L)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
207 <param name="map1NumEdits" type="float" value="-1" label="The maximum number of edits or false-negative probability assuming the maximum error rate (-1 to auto-tune) (-p)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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208 <param name="map1BaseError" type="float" value="0.02" label="The the assumed per-base maximum error rate (-P)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
209 <param name="map1Mismatches" type="float" value="3" label="The maximum number of or (read length) fraction of mismatches (-m)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
210 <param name="map1GapOpens" type="float" value="1" label="The maximum number of or (read length) fraction of indel starts (-o)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
211 <param name="map1GapExtensions" type="float" value="6" label="The maximum number of or (read length) fraction of indel extensions (-e)" />
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diff changeset
212 <param name="map1MaxCALsDeletion" type="integer" value="10" label="The maximum number of CALs to extend a deletion (-d)" />
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213 <param name="map1EndIndels" type="integer" value="5" label="Indels are not allowed within this number of bps from the end of the read (-i)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
214 <param name="map1MaxOptimalCALs" type="integer" value="32" label="Stop searching when INT optimal CALs have been found (-b)" />
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215 <param name="map1MaxNodes" type="integer" value="2000000" label="The maximum number of alignment nodes (-Q)" />
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216 </when>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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217 <when value="false" />
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diff changeset
218 </conditional>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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219 <conditional name="map2">
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220 <param name="source_select" type="select" label="Map2 Stage One" help="Turn on mapping algorithm #2 in the first stage">
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221 <option value="true">Turn On</option>
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222 <option value="false" selected="true">Turn Off</option>
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diff changeset
223 </param>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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224 <when value="true">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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225 <param name="map2Coefficient" type="float" value="5.5" label="The coefficient of length-threshold adjustment (-c)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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226 <param name="map2SeedIntervalSize" type="integer" value="3" label="The maximum seeding interval size (-S)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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227 <param name="map2ZBest" type="integer" value="1" label="Keep the z-best nodes during prefix trie traversal (-b)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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228 <param name="map2ReverseTrigger" type="integer" value="5" label="The # seeds to trigger reverse alignment (-N)" />
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229 </when>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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230 <when value="false" />
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diff changeset
231 </conditional>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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232 <conditional name="map3">
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233 <param name="source_select" type="select" label="Map3 Stage One" help="Turn on mapping algorithm #3 in the first stage">
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234 <option value="true">Turn On</option>
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235 <option value="false" selected="true">Turn Off</option>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
236 </param>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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237 <when value="true">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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238 <param name="map3SeedLength" type="integer" value="-1" label="The k-mer length to seed CALs (-1 tunes to the genome size) (-l)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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239 <param name="map3SeedMaxHits" type="integer" value="12" label="The maximum number of hits returned by a seed (-S)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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240 <param name="map3SeedWindow" type="integer" value="25" label="The window of bases in which to group seeds (-b)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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241 <param name="map3HPEnumeration" type="integer" value="0" label="The single homopolymer error difference for enumeration (-H)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
242 </when>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
243 <when value="false" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
244 </conditional>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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245 <conditional name="MAP1">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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246 <param name="source_select" type="select" label="Map1 Stage Two" help="Turn on mapping algorithm #1 in the second stage">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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247 <option value="true">Turn On</option>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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248 <option value="false" selected="true">Turn Off</option>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
249 </param>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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250 <when value="true">
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
251 <param name="MAP1SeedLength" type="integer" value="32" label="The k-mer length to seed CALs (-1 to disable) (-l)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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252 <param name="MAP1SeedMismatches" type="integer" value="2" label="The maximum number of mismatches in the seed (-s)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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253 <param name="MAP1SecondarySeedLength" type="integer" value="64" label="The secondary seed length (-1 to disable) (-L)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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254 <param name="MAP1NumEdits" type="float" value="-1" label="The maximum number of edits or false-negative probability assuming the maximum error rate (-1 to auto-tune) (-p)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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255 <param name="MAP1BaseError" type="float" value="0.02" label="The the assumed per-base maximum error rate (-P)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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256 <param name="MAP1Mismatches" type="float" value="3" label="The maximum number of or (read length) fraction of mismatches (-m)" />
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257 <param name="MAP1GapOpens" type="float" value="1" label="The maximum number of or (read length) fraction of indel starts (-o)" />
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258 <param name="MAP1GapExtensions" type="float" value="6" label="The maximum number of or (read length) fraction of indel extensions (-e)" />
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259 <param name="MAP1MaxCALsDeletion" type="integer" value="10" label="The maximum number of CALs to extend a deletion (-d)" />
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260 <param name="MAP1EndIndels" type="integer" value="5" label="Indels are not allowed within this number of bps from the end of the read (-i)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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diff changeset
261 <param name="MAP1MaxOptimalCALs" type="integer" value="32" label="Stop searching when INT optimal CALs have been found (-b)" />
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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262 <param name="MAP1MaxNodes" type="integer" value="2000000" label="The maximum number of alignment nodes (-Q)" />
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263 </when>
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264 <when value="false" />
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265 </conditional>
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266 <conditional name="MAP2">
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267 <param name="source_select" type="select" label="Map2 Stage Two" help="Turn on mapping algorithm #2 in the second stage">
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268 <option value="true">Turn On</option>
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269 <option value="false" selected="true">Turn Off</option>
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270 </param>
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271 <when value="true">
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272 <param name="MAP2Coefficient" type="float" value="5.5" label="The coefficient of length-threshold adjustment (-c)" />
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273 <param name="MAP2SeedIntervalSize" type="integer" value="3" label="The maximum seeding interval size (-S)" />
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274 <param name="MAP2ZBest" type="integer" value="1" label="Keep the z-best nodes during prefix trie traversal (-b)" />
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275 <param name="MAP2ReverseTrigger" type="integer" value="5" label="The # seeds to trigger reverse alignment (-N)" />
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276 </when>
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277 <when value="false" />
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278 </conditional>
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279 <conditional name="MAP3">
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280 <param name="source_select" type="select" label="Map3 Stage Two" help="Turn on mapping algorithm #3 in the second stage">
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281 <option value="true">Turn On</option>
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282 <option value="false" selected="true">Turn Off</option>
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283 </param>
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284 <when value="true">
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285 <param name="MAP3SeedLength" type="integer" value="-1" label="The k-mer length to seed CALs (-1 tunes to the genome size) (-l)" />
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286 <param name="MAP3SeedMaxHits" type="integer" value="12" label="The maximum number of hits returned by a seed (-S)" />
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287 <param name="MAP3SeedWindow" type="integer" value="25" label="The window of bases in which to group seeds (-b)" />
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288 <param name="MAP3HPEnumeration" type="integer" value="0" label="The single homopolymer error difference for enumeration (-H)" />
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289 </when>
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290 <when value="false" />
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291 </conditional>
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292 </when>
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293 </conditional>
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294 <param name="suppressHeader" type="boolean" truevalue="true" falsevalue="false" checked="true" label="Suppress the header in the output SAM file" help="TMAP produces SAM with several lines of header information" />
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295 </inputs>
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296 <outputs>
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297 <data format="sam" name="output" label="${tool.name} on ${on_string}: mapped reads">
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298 <actions>
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299 <conditional name="genomeSource.refGenomeSource">
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300 <when value="indexed">
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301 <action type="metadata" name="dbkey">
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302 <option type="from_data_table" name="tmap_indexes" column="0">
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303 <filter type="param_value" ref="genomeSource.indices" column="1"/>
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304 </option>
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305 </action>
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306 </when>
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307 </conditional>
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308 </actions>
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309 </data>
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310 </outputs>
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311 <help>
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312
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313 **What it does**
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314
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315 TMAP is a fast light-weight tool that aligns DNA sequences (small queries) to a sequence database (large sequences), such as the human reference genome. TMAP follows a two-stage approach, with a set of algorithms and associated settings for each stage. If there are no mappings for a read by applying the algorithms in the first stage, then the algorithms in the second stage are applied. For example, a set of algorithms to quickly align near-perfect reads may be used in the first stage, while a set of sensitive algorithms may be used to map difficult reads in the second stage.
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316
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317 It combines multiple mapping algorithms to give sensitive and accurate alignments quickly. It uses three core algorithms, BWA-short, BWA-long, and a variant of the SSAHA algorithm. These algorithms are described in the following publications:
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318 - Li, H. and Durbin, R. (2009). Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics, 25, 1754–1760.
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319 - Li, H. and Durbin, R. (2010). Fast and accurate long-read alignment with Burrows-Wheeler
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320 - Ning, Z., Cox, A., and Mullikin, J. (2001). SSAHA: a fast search method for large DNA databases. Genome Res., 11, 1725–1729.
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321
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322 ------
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323
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324 **Know what you are doing**
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325
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326 .. class:: warningmark
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327
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328 There is no such thing (yet) as an automated gearshift in short read mapping. It is all like stick-shift driving in San Francisco. In other words = running this tool with default parameters will probably not give you meaningful results. A way to deal with this is to **understand** the parameters by carefully reading the `documentation`__ and experimenting. Fortunately, Galaxy makes experimenting easy.
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329
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330 .. __: http://www.iontorrent.com/
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331
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332 ------
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333
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334 **Input formats**
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335
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336 TMAP accepts files in Sanger FASTQ format. Use the FASTQ Groomer to prepare your files.
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337
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338 ------
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339
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340 **A Note on Built-in Reference Genomes**
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341
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342 Some genomes have multiple variants. If only one "type" of genome is listed, it is the Full version, which means that everything that came in the original genome data download (possibly with mitochondrial and plasmid DNA added if it wasn't already included). The Full version is available for every genome. Some genomes also come in the Canonical variant, which contains only the "canonical" (well-defined) chromosomes or segments, such as chr1-chr22, chrX, chrY, and chrM for human. Other variations include gender. These will come in the canonical form only, so the general Canonical variant is actually Canonical Female and the other is Canonical Male (identical to female excluding chrX).
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343
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344 ------
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345
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346 **Outputs**
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347
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348 The output is in SAM format, and has the following columns::
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349
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350 Column Description
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351 -------- --------------------------------------------------------
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352 1 QNAME Query (pair) NAME
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353 2 FLAG bitwise FLAG
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354 3 RNAME Reference sequence NAME
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355 4 POS 1-based leftmost POSition/coordinate of clipped sequence
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356 5 MAPQ MAPping Quality (Phred-scaled)
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357 6 CIGAR extended CIGAR string
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358 7 MRNM Mate Reference sequence NaMe ('=' if same as RNAME)
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359 8 MPOS 1-based Mate POSition
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360 9 ISIZE Inferred insert SIZE
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361 10 SEQ query SEQuence on the same strand as the reference
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362 11 QUAL query QUALity (ASCII-33 gives the Phred base quality)
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363 12 OPT variable OPTional fields in the format TAG:VTYPE:VALU
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364
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365 The flags are as follows::
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366
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367 Flag Description
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368 ------ -------------------------------------
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369 0x0001 the read is paired in sequencing
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370 0x0002 the read is mapped in a proper pair
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371 0x0004 the query sequence itself is unmapped
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372 0x0008 the mate is unmapped
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373 0x0010 strand of the query (1 for reverse)
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374 0x0020 strand of the mate
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375 0x0040 the read is the first read in a pair
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376 0x0080 the read is the second read in a pair
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377 0x0100 the alignment is not primary
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378
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379 It looks like this (scroll sideways to see the entire example)::
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380
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381 QNAME FLAG RNAME POS MAPQ CIAGR MRNM MPOS ISIZE SEQ QUAL OPT
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382 HWI-EAS91_1_30788AAXX:1:1:1761:343 4 * 0 0 * * 0 0 AAAAAAANNAAAAAAAAAAAAAAAAAAAAAAAAAAACNNANNGAGTNGNNNNNNNGCTTCCCACAGNNCTGG hhhhhhh;;hhhhhhhhhhh^hOhhhhghhhfhhhgh;;h;;hhhh;h;;;;;;;hhhhhhghhhh;;Phhh
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383 HWI-EAS91_1_30788AAXX:1:1:1578:331 4 * 0 0 * * 0 0 GTATAGANNAATAAGAAAAAAAAAAATGAAGACTTTCNNANNTCTGNANNNNNNNTCTTTTTTCAGNNGTAG hhhhhhh;;hhhhhhhhhhhhhhhhhhhhhhhhhhhh;;h;;hhhh;h;;;;;;;hhhhhhhhhhh;;hhVh
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384
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385 -------
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386
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387 **TMAP settings**
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388
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389 All of the options have a default value. You can change most of them. Most of the options in TMAP have been implemented here.
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390
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391 ------
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392
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393 **TMAP parameter list**
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394
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395 This is an exhaustive list of TMAP options implemented here:
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396
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397 For the **global** options::
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398
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399 -A INT score for a match [5]
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400 -M INT the mismatch penalty [3]
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401 -O INT the indel start penalty [3]
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402 -E INT the indel extend penalty [1]
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403 -X INT the flow score penalty [7]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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404 -w INT the band width [50]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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405 -g map the full read [false]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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406 -W INT remove duplicate alignments from different algorithms within this bp window (-1 to disable) [128]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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407 -T INT score threshold divided by the match score [30]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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408 -q INT the queue size for the reads (-1 disables) [65536]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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409 -n INT the number of threads [1]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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410 -a INT output filter [1]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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411 0 - unique best hits
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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412 1 - random best hit
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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413 2 - all best hits
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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414 3 - all alignments
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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415 -R STRING the RG tags to add to the SAM header [(null)]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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416
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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417 For **mapall** options::
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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418
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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419 -I apply the output filter for each algorithm separately [false]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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420
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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421 For **map1** options::
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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422
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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423 -l INT the k-mer length to seed CALs (-1 to disable) [32]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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424 -s INT maximum number of mismatches in the seed [3]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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425 -m NUM maximum number of or (read length) fraction of mismatches [number: 2]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
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426 -o NUM maximum number of or (read length) fraction of indel starts [number: 1]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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427 -e NUM maximum number of or (read length) fraction of indel extensions [number: 6]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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428 -d INT the maximum number of CALs to extend a deletion [10]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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429 -i INT indels are not allowed within INT number of bps from the end of the read [5]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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430 -b INT stop searching when INT optimal CALs have been found [32]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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431 -Q INT maximum number of alignment nodes [2000000]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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432
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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433 For **map2** options::
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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diff changeset
434
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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435 -c FLOAT coefficient of length-threshold adjustment [5.5]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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436 -S INT maximum seeding interval size [3]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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437 -b INT Z-best [1]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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438 -N INT # seeds to trigger reverse alignment [5]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
diff changeset
439
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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440 For **map3** options::
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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441
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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442 -l INT the k-mer length to seed CALs (-1 tunes to the genome size) [-1]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
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443 -S INT the maximum number of hits returned by a seed [8]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
parents:
diff changeset
444 -b INT the window of bases in which to group seeds [50]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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445 -H INT single homopolymer error difference for enumeration [0]
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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446
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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447 </help>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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448 </tool>
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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449
7cbfd271d207 Migrated tool version 0.0.19 from old tool shed archive to new tool shed repository
nilshomer
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450