# HG changeset patch
# User ning
# Date 1363385988 14400
# Node ID aebe98ba5c22a20bf9643d80efa8ad432916fea5
# Parent  e42c12a40df6e464ef20ea62a594b7377d649888
Uploaded

diff -r e42c12a40df6 -r aebe98ba5c22 GetNormalizedExpression.R
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/GetNormalizedExpression.R	Fri Mar 15 18:19:48 2013 -0400
@@ -0,0 +1,41 @@
+sink(file="/tmp/none")
+sink("/dev/null")
+options(warn=-1)
+options(echo=F) 
+
+invisible("EBSeq")
+suppressMessages(library("EBSeq"))
+
+args <- commandArgs(trailingOnly = T)
+inputfile <- args[1]
+WhetherSampleName <- args[2]
+outputfile <- args[3]
+Boxplots<-args[4]
+Sizesout <-args[5]
+
+print(args)
+
+if(WhetherSampleName=="y"){
+	ReadIn=read.table(inputfile,stringsAsFactors=F,header=T, sep="\t")
+	Names=names(ReadIn)[-1]
+	}
+if(WhetherSampleName=="n"){
+	ReadIn=read.table(inputfile,stringsAsFactors=F,header=F, sep="\t")
+}
+
+GeneMat=do.call(cbind,ReadIn[-1])
+rownames(GeneMat)=ReadIn[[1]]
+if(WhetherSampleName=="y")colnames(GeneMat)=Names
+
+
+Sizes=MedianNorm(GeneMat)
+
+Data.norm=GetNormalizedMat(GeneMat, Sizes)
+
+write.table(round(Data.norm,2),file=outputfile,quote=F,col.names=T,row.names=T,sep = "\t")
+pdf(Boxplots)
+boxplot(Data.norm,log="y",ylim=c(10^-1,10^6))
+dev.off()
+
+write.table(Sizes,file=Sizesout,quote=F,col.names=F,row.names=F,sep = "\t")
+