Mercurial > repos > ning > ebseq
changeset 13:4d6d52fafb86 draft
Uploaded
author | ning |
---|---|
date | Fri, 15 Mar 2013 18:23:26 -0400 |
parents | 1108020e2d84 |
children | eb8e01ed3516 |
files | EBGeneMultiCondTest.R |
diffstat | 1 files changed, 65 insertions(+), 0 deletions(-) [+] |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/EBGeneMultiCondTest.R Fri Mar 15 18:23:26 2013 -0400 @@ -0,0 +1,65 @@ +sink(file="/tmp/none") +sink("/dev/null") +options(warn=-1) +options(echo=F) + +invisible("EBSeq") +suppressMessages(library("EBSeq")) + +args <- commandArgs(trailingOnly = T) +inputfile <- args[1] +WhetherSampleName <- args[2] +CondIn <- args[3] +PatternFile <- args[4] +outputfile <- args[5] +MAP.out<-args[6] +Sizesout <-args[7] + +#write.table(PatternFile,outputfile,quote=F,col.names=T,row.names=T,sep = "\t") + +#write.table(args,outputfile,quote=F,col.names=T,row.names=T,sep = "\t") + +Conditions=strsplit(CondIn,split=",")[[1]] + +if(WhetherSampleName=="y"){ + ReadIn=read.table(inputfile,stringsAsFactors=F,header=T, sep="\t") + Names=names(ReadIn)[-1] + } +if(WhetherSampleName=="n"){ + ReadIn=read.table(inputfile,stringsAsFactors=F,header=F, sep="\t") + Names=paste0("S",1:length(Conditions)) +} + +PatternIn=read.table(PatternFile,stringsAsFactors=F,header=T,sep="\t") + +if(class(ReadIn[[1]])=="character"){ + GeneMat=do.call(cbind,ReadIn[-1]) + rownames(GeneMat)=ReadIn[[1]] + colnames(GeneMat)=Names +} +if(class(ReadIn[[1]])=="numeric"){ + GeneMat=data.matrix(ReadIn) + colnames(GeneMat)=Names + } + +Patterns=data.matrix(PatternIn) + + +Sizes=MedianNorm(GeneMat) +EBOut=EBMultiTest(Data=GeneMat,Conditions=as.factor(Conditions), + AllParti=Patterns,sizeFactors=Sizes, maxround=5) +PPout=GetMultiPP(EBOut) +MultiPP=PPout$PP +MultiMAP=PPout$MAP +Data.norm=round(GetNormalizedMat(GeneMat, Sizes),2) + +Mat=cbind(MultiMAP,Data.norm[names(MultiMAP),]) + +colnames(Mat)= +c("MAP",Names) +options(warn=-1) + +write.table(round(MultiPP,2),file=outputfile,quote=F,col.names=T,row.names=T,sep = "\t") +write.table(Mat,file=MAP.out ,quote=F,col.names=T,row.names=T,sep = "\t") +write.table(Sizes,file=Sizesout,quote=F,col.names=F,row.names=F,sep = "\t") +