Mercurial > repos > nml > bio_hansel
diff bio_hansel.xml @ 3:29faaa849e41 draft
planemo upload for repository https://github.com/phac-nml/bio_hansel commit 4f09991d49a1701c54d5b97601a622e111c7290c
author | nml |
---|---|
date | Fri, 22 Dec 2017 10:31:41 -0500 |
parents | 09ebaa5192ab |
children | c0d08ba0c6ee |
line wrap: on
line diff
--- a/bio_hansel.xml Fri Oct 27 12:40:00 2017 -0400 +++ b/bio_hansel.xml Fri Dec 22 10:31:41 2017 -0500 @@ -1,7 +1,7 @@ -<tool id="bio_hansel" name="Salmonella Subtyping" version="0.1.3"> - <description>Genome assemblies and/or whole-genome sequencing readset</description> +<tool id="bio_hansel" name="Bio Hansel" version="1.0.0"> + <description>SNV Subtyping with genome assemblies or reads</description> <requirements> - <requirement type="package" version="0.1.0">bio_hansel</requirement> + <requirement type="package" version="1.1.0">bio_hansel</requirement> <requirement type="package" version="17.2.0">attrs</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ @@ -52,16 +52,32 @@ #end if - #if $kmer_min - --min-kmer-freq $kmer_min + #if $kmer_vals.kmer_min + --min-kmer-freq $kmer_vals.kmer_min + #end if + + #if $kmer_vals.kmer_max + --max-kmer-freq $kmer_vals.kmer_max #end if - #if $kmer_max - --max-kmer-freq $kmer_max + #if $qc_vals.low_cov_depth_freq + --low-cov-depth-freq $qc_vals.low_cov_depth_freq + #end if + + #if $qc_vals.max_missing_tiles + --max-missing-tiles $qc_vals.max_missing_tiles + #end if + + #if $qc_vals.min_ambiguous_tiles + --min-ambiguous-tiles $qc_vals.min_ambiguous_tiles + #end if + + #if $qc_vals.max_intermediate_tiles + --max-intermediate-tiles $qc_vals.max_intermediate_tiles #end if ## Adding more parameters to the command. - -vvv -t "\${GALAXY_SLOTS:-1}" -o results.tab -O match_results.tab + -vvv -t "\${GALAXY_SLOTS:-1}" -o results.tab -O match_results.tab -S tech_results.tab ## Entering the file inputs @@ -109,12 +125,23 @@ <param name="scheme_input" type="data" format="fasta" label="Scheme Input"/> </when> </conditional> - <param name="kmer_min" argument="--min-kmer-freq" optional="True" type="integer" min="0" label="Min k-mer freq/coverage" value="10" help="default = 10"/> - <param name="kmer_max" argument="--max-kmer-freq" optional="True" type="integer" min="1" label="Max k-mer freq/coverage" value="200" help="default = 200"/> + <!-- K-mer frequencies. --> + <section name="kmer_vals" title="K-mer Frequency Thresholds" expanded="False"> + <param name="kmer_min" argument="--min-kmer-freq" optional="True" type="integer" min="0" label="Min k-mer freq/coverage" value="8" help="default = 8"/> + <param name="kmer_max" argument="--max-kmer-freq" optional="True" type="integer" min="1" label="Max k-mer freq/coverage" value="1000" help="default = 1000"/> + </section> + <!-- Quality Checking Parameters --> + <section name="qc_vals" title="Quality Checking Thresholds" expanded="False"> + <param name="low_cov_depth_freq" argument="--low-cov-depth-freq" optional="True" type="integer" min="0" label="QC: Frequency below this coverage are considered low coverage" value="20" help="default = 20"/> + <param name="min_ambiguous_tiles" argument="--min-ambiguous-tiles" optional="True" type="integer" min="0" label="QC: Min number of tiles missing for Ambiguous Result" value="3" help="default = 3"/> + <param name="max_missing_tiles" argument="--max-missing-tiles" optional="True" type="float" min="0" max="1" label="QC: Decimal Proportion of max allowed missing tiles" value="0.05" help="default = 0.05, valid values {0.0 - 1.0}"/> + <param name="max_intermediate_tiles" argument="--max-intermediate-tiles" optional="True" type="float" min="0" max="1" label="QC: Decimal Proportion of max allowed missing tiles for an intermediate subtype" value="0.05" help="default = 0.05, valid values {0.0 - 1.0}"/> + </section> </inputs> <outputs> <data format="tabular" name="results.tab" from_work_dir="results.tab" label="results.tab"/> <data format="tabular" name="match_results.tab" from_work_dir="match_results.tab" label="match_results.tab"/> + <data format="tabular" name="tech_results.tab" from_work_dir="tech_results.tab" label="tech_results.tab"/> </outputs> <tests> <test> @@ -123,16 +150,17 @@ <param name="fastq_input1" value="SRR1002850_SMALL.fasta"/> <output name="results.tab"> <assert_contents> - <!-- Verifying that the columns are as expected. --> - <has_text_matching expression="sample\s+scheme\s+subtype\s+all_subtypes\s+tiles_matching_subtype\s+are_subtypes_consistent\s+inconsistent_subtypes\s+n_tiles_matching_all\s+n_tiles_matching_all_total\s+n_tiles_matching_positive\s+n_tiles_matching_positive_total\s+n_tiles_matching_subtype\s+n_tiles_matching_subtype_total\s+file_path"/> - <!-- Verifying that the output of running the test file is expected. This is done via REGEX because the name and path of the file outputted to results.tab changes each test. --> - <has_text_matching expression="(heidelberg)\s+(2.2.2.2.1.4)\s+(2;)\s+(2.2;)\s+(2.2.2;)\s+(2.2.2.2;)\s+(2.2.2.2.1;)\s+(2.2.2.2.1.4)\s+(1037658-2.2.2.2.1.4;)\s+(2154958-2.2.2.2.1.4;)\s+(3785187-2.2.2.2.1.4)\s+(True)\s+(202)\s+(202)\s+(17)\s+(17)\s+(3)\s+(3)"/> + <has_text_matching expression="sample\s+scheme\s+scheme_version\s+subtype\s+all_subtypes\s+tiles_matching_subtype\s+are_subtypes_consistent\s+inconsistent_subtypes\s+n_tiles_matching_all\s+n_tiles_matching_all_expected\s+n_tiles_matching_positive\s+n_tiles_matching_positive_expected\s+n_tiles_matching_subtype\s+n_tiles_matching_subtype_expected\s+file_path\s+qc_status\s+qc_message"/> </assert_contents> </output> <output name="match_results.tab"> <assert_contents> - <!-- This is the last line in the file, this assertion is to make sure that we have the correct number of items. --> - <has_text_matching expression="negative4738855-1.1"/> + <has_text_matching expression="tilename\s+seq\s+is_revcomp\s+contig_id\s+match_index\s+refposition\s+subtype\s+is_pos_tile\s+sample\s+file_path\s+scheme\s+scheme_version\s+qc_status\s+qc_message"/> + </assert_contents> + </output> + <output name="tech_results.tab"> + <assert_contents> + <has_text_matching expression="sample\s+subtype\s+qc_status\s+qc_message"/> </assert_contents> </output> </test> @@ -143,10 +171,17 @@ <param name="fastq_input2" value="SRR5646583_SMALL_2.fastq"/> <output name="results.tab"> <assert_contents> - <!-- Verifying that the columns are as expected. --> - <has_text_matching expression="sample\s+scheme\s+subtype\s+all_subtypes\s+tiles_matching_subtype\s+are_subtypes_consistent\s+inconsistent_subtypes\s+n_tiles_matching_all\s+n_tiles_matching_all_total\s+n_tiles_matching_positive\s+n_tiles_matching_positive_total\s+n_tiles_matching_subtype\s+n_tiles_matching_subtype_total\s+file_path"/> - <!-- Verifying that the output of running the test file is expected. This is done via REGEX because the name and path of the file outputted to results.tab changes each test. --> - <has_text_matching expression="(heidelberg)\s+(2.2.1.1.1.1)\s+(2;)\s+(2.2;)\s+(2.2.1;)\s+(2.2.1.1;)\s+(2.2.1.1.1;)\s+(2.2.1.1.1.1)\s+(1983064-2.2.1.1.1.1;)\s+(4211912-2.2.1.1.1.1)\s+(True)\s+(202)\s+(202)\s+(20)\s+(20)\s+(2)\s+(2)"/> + <has_text_matching expression="sample\s+scheme\s+scheme_version\s+subtype\s+all_subtypes\s+tiles_matching_subtype\s+are_subtypes_consistent\s+inconsistent_subtypes\s+n_tiles_matching_all\s+n_tiles_matching_all_expected\s+n_tiles_matching_positive\s+n_tiles_matching_positive_expected\s+n_tiles_matching_subtype\s+n_tiles_matching_subtype_expected\s+file_path\s+qc_status\s+qc_message"/> + </assert_contents> + </output> + <output name="match_results.tab"> + <assert_contents> + <has_text_matching expression="tilename\s+seq\s+freq\s+refposition\s+subtype\s+is_pos_tile\s+is_kmer_freq_okay\s+sample\s+scheme\s+scheme_version\s+qc_status\s+qc_message"/> + </assert_contents> + </output> + <output name="tech_results.tab"> + <assert_contents> + <has_text_matching expression="sample\s+subtype\s+qc_status\s+qc_message"/> </assert_contents> </output> </test>