Mercurial > repos > nml > metaspades
diff metaspades.xml @ 0:e93c1a0678cd draft
planemo upload commit 18fef9393a17a3442ab7927d76b301bb43ec3de4
author | nml |
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date | Tue, 09 Aug 2016 10:46:28 -0400 |
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children | 01a241476407 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/metaspades.xml Tue Aug 09 10:46:28 2016 -0400 @@ -0,0 +1,140 @@ +<tool id="metaspades" name="metaspades" version="1.0"> + <description>genome assembler for metagenomics datasets</description> + <requirements> + <requirement type="package" version="3.9.0">spades</requirement> + </requirements> + <command interpreter="perl">spades.pl + $out_contigs + $out_contig_stats + $out_scaffolds + $out_scaffold_stats + $out_log + + ## if the first fileset is a paired-collection, use the key as the name + #if $files[0].file_type.type == "paired-collection": + $files[0].file_type.fastq_collection.name + #else: + NODE + #end if + ## A real command looks like: spades.py -k 21,33,55,77,99,127 --careful -1 Y.fastq.gz -2 X.fastq.gz -t 24 -o output + spades.py + ## Forces unzipped output, faster + --disable-gzip-output + --meta + $onlyassembler + + -t \${GALAXY_SLOTS:-16} + + #if not $kmer_choice.auto_kmer_choice: + -k "$kmer_choice.kmers" + #end if + + ## Sequence files + #set num=1 + #if str( $lib_type ) == "paired_end": + #set prefix = 'pe' + #end if + --$prefix$num-$orientation + #for $file in $files + #if $file.file_type.type == "separate" + --$prefix$num-1 fastq:$file.file_type.fwd_reads + --$prefix$num-2 fastq:$file.file_type.rev_reads + #elif $file.file_type.type == "interleaved" + --$prefix$num-12 fastq:$file.file_type.interleaved_reads + #elif $file.file_type.type == "paired-collection" + --$prefix$num-1 fastq:$file.file_type.fastq_collection.forward + --$prefix$num-2 fastq:$file.file_type.fastq_collection.reverse + #end if + #end for + + + </command> + <inputs> + <param name="onlyassembler" type="boolean" truevalue="--only-assembler" falsevalue="" checked="False" label="Run only assembly? (without read error correction)" /> + <conditional name="kmer_choice"> + <param name="auto_kmer_choice" type="boolean" checked="False" truevalue="true" falsevalue="false" label="Automatically choose k-mer values" help="k-mer choices can be chosen by SPAdes instead of being entered manually" /> + <when value="false"> + <param name="kmers" type="text" label="K-mers to use, separated by commas" value="21,33,55" help="Comma-separated list of k-mer sizes to be used (all values must be odd, less than 128, listed in ascending order, and smaller than the read length). The default value is 21,33,55." /> + </when> + <when value="true"> </when> + </conditional> + + <!-- Reads --> + + <param name="lib_type" type="select" label="Library type"> + <option value="paired_end">Paired-end</option> + </param> + <param name="orientation" type="select" label="Orientation"> + <option value="fr" selected="true">-> <- (fr)</option> + <option value="rf"><- -> (rf)</option> + <option value="ff">-> -> (ff)</option> + </param> + <repeat name="files" title="Files" min="1"> + <conditional name="file_type"> + <param name="type" type="select" label="Select file format"> + <option value="separate">Separate input files</option> + <option value="interleaved">Interleaved files</option> + <option value="paired-collection">Paired List Collection</option> + </param> + <when value="separate"> + <param name="fwd_reads" type="data" format="fastq" label="Forward reads" help="FASTQ format" /> + <param name="rev_reads" type="data" format="fastq" label="Reverse reads" help="FASTQ format" /> + </when> + <when value="interleaved"> + <param name="interleaved_reads" type="data" format="fastq" label="Interleaved paired reads" help="FASTQ format" /> + </when> + <when value="paired-collection"> + <param name="fastq_collection" type="data_collection" label="Paired-end reads collection" optional="false" format="fastq" collection_type="paired" help="FASTQ format" /> + </when> + </conditional> + </repeat> + + + </inputs> + <outputs> + <data name="out_contigs" format="fasta" label="SPAdes contigs (fasta)" /> + <data name="out_contig_stats" format="tabular" label="SPAdes contig stats" /> + <data name="out_scaffolds" format="fasta" label="SPAdes scaffolds (fasta)" /> + <data name="out_scaffold_stats" format="tabular" label="SPAdes scaffold stats" /> + <data name="out_log" format="txt" label="SPAdes log" /> + </outputs> + <tests> + <test> + <param name="sc" value="false" /> + <param name="careful" value="false" /> + <param name="kmers" value="33,55" /> + <param name="lib_type" value="paired_end" /> + <param name="fwd_reads" value="ecoli_1K_1.fq" ftype="fastq" /> + <param name="rev_reads" value="ecoli_1K_2.fq" ftype="fastq" /> + <output name="out_contigs" file="reference_1K.fa" ftype="fasta" compare="re_match" lines_diff="1" /> + </test> + </tests> + <help> +**What it does** + +SPAdes – St. Petersburg genome assembler – is intended for both standard isolates and single-cell MDA bacteria assemblies. See http://bioinf.spbau.ru/en/spades for more details on SPAdes. + +This wrapper runs SPAdes 3.9, collects the output, and throws away all the temporary files. It also produces a tab file with contig names, length and coverage. + +**License** + +SPAdes is developed by and copyrighted to Saint-Petersburg Academic University, and is released under GPLv2. + +This wrapper is copyrighted by Philip Mabon and is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. + +This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details. + +You should have received a copy of the GNU General Public License along with this program. If not, see http://www.gnu.org/licenses/. + +** Acknowledgments ** + +Original wrapper developed by Lionel Guy. + +Anton Korobeynikov greatlty helped understanding how SPAdes work, and integrated handy features into SPAdes. + +Nicola Soranzo fixed various bugs. + </help> + <citations> + <citation type="doi">10.1089/cmb.2012.0021</citation> + </citations> +</tool>