Mercurial > repos > nml > pseudogenome
diff pseudogenome.xml @ 0:47b586ab4729 draft default tip
planemo upload commit 4fee4519135f7677cf50f721cf1ad7a7335ad66d-dirty
author | nml |
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date | Fri, 06 Apr 2018 14:29:17 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/pseudogenome.xml Fri Apr 06 14:29:17 2018 -0400 @@ -0,0 +1,73 @@ +<tool id="pseudogenome" name="Create pseudo genome" version="1.0.0"> + <description>from a fasta file in order of appearance</description> + <requirements> + <requirement type="package" version="1.6.924">perl-bioperl</requirement> + <requirement type="package" version="1.04">perl-readonly</requirement> + <requirement type="package" version="2.49">perl-getopt-long</requirement> + <requirement type="package" version="1.25">perl-ipc-system-simple</requirement> + </requirements> + <command detect_errors="exit_code"><![CDATA[ + perl '$__tool_directory__/pseudogenome.pl' -i '$input' + + #if $stitch.howto == "jcvi": + -s + #else + -n '$stitch.number' -c '$stitch.glue' + #end if + + -o '$output' + ]]></command> + <inputs> + <param name="input" type="data" format="fasta" label="Multi contig fasta file" optional="false"/> + + <conditional name="stitch"> + <param name="howto" type="select" label="How do you want to merge contigs?"> + <option selected="true" value="jcvi">JCVI Linker</option> + <option value="custom">Custom options</option> + </param> + <when value="jcvi"> + </when> + <when value="custom"> + <param name="number" type="integer" value="10" label="Number of filler base pairs" optional="false"/> + <param name="glue" type="text" value="N" label="Character inserted between contigs" optional="false"/> + </when> + </conditional> + </inputs> + <outputs> + <data format="fasta" name="output"/> + </outputs> + <tests> + <test> + <param name="input" value="input.fasta"/> + <output name="output" value="output.fasta"/> + </test> + <test> + <param name="input" value="input.fasta"/> + <param name="howto" value="custom"/> + <param name="number" value="50"/> + <param name="glue" value="X"/> + <output name="output" value="custom.fasta"/> + </test> + </tests> + <help> + +What it does +============ +This tool takes in a mult-contig fasta file and converts it into a pseudo genome. + + + +JCVI Linker +============ + +Linker is a 36 base pair sequence which places start and +stop codons in all 6 reading frames to prevent gene for being predicted across contigs. + +Sequence below: + +"NNNNNCACACACTTAATTAATTAAGTGTGTGNNNNN" + + </help> + <citations> + </citations> +</tool>