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author | nml |
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date | Fri, 06 Apr 2018 14:29:17 -0400 |
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<tool id="pseudogenome" name="Create pseudo genome" version="1.0.0"> <description>from a fasta file in order of appearance</description> <requirements> <requirement type="package" version="1.6.924">perl-bioperl</requirement> <requirement type="package" version="1.04">perl-readonly</requirement> <requirement type="package" version="2.49">perl-getopt-long</requirement> <requirement type="package" version="1.25">perl-ipc-system-simple</requirement> </requirements> <command detect_errors="exit_code"><![CDATA[ perl '$__tool_directory__/pseudogenome.pl' -i '$input' #if $stitch.howto == "jcvi": -s #else -n '$stitch.number' -c '$stitch.glue' #end if -o '$output' ]]></command> <inputs> <param name="input" type="data" format="fasta" label="Multi contig fasta file" optional="false"/> <conditional name="stitch"> <param name="howto" type="select" label="How do you want to merge contigs?"> <option selected="true" value="jcvi">JCVI Linker</option> <option value="custom">Custom options</option> </param> <when value="jcvi"> </when> <when value="custom"> <param name="number" type="integer" value="10" label="Number of filler base pairs" optional="false"/> <param name="glue" type="text" value="N" label="Character inserted between contigs" optional="false"/> </when> </conditional> </inputs> <outputs> <data format="fasta" name="output"/> </outputs> <tests> <test> <param name="input" value="input.fasta"/> <output name="output" value="output.fasta"/> </test> <test> <param name="input" value="input.fasta"/> <param name="howto" value="custom"/> <param name="number" value="50"/> <param name="glue" value="X"/> <output name="output" value="custom.fasta"/> </test> </tests> <help> What it does ============ This tool takes in a mult-contig fasta file and converts it into a pseudo genome. JCVI Linker ============ Linker is a 36 base pair sequence which places start and stop codons in all 6 reading frames to prevent gene for being predicted across contigs. Sequence below: "NNNNNCACACACTTAATTAATTAAGTGTGTGNNNNN" </help> <citations> </citations> </tool>