--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/callaavar.xml	Mon Dec 04 10:25:26 2017 -0500
@@ -0,0 +1,59 @@
+<tool id="aavariants" name="Amino Acid Variants" version="0.1.0">
+    <description>Identifies amino acid mutations</description>
+    <requirements>
+        <requirement type="package" version="0.2.2">quasitools</requirement>
+    </requirements>
+    <command detect_errors="exit_code"><![CDATA[
+
+        ln -f -s ${input_bam.metadata.bam_index} ${input_bam}.bai &&
+        quasitools call aavar $input_bam $ref_file $var_file $input_genes 
+
+        #if $mutation_db:
+          $mutation_db 
+        #end if
+
+        -o output.vcf
+
+    ]]></command>
+    <inputs>
+        <param name="input_bam" type="data" format="bam" optional="false" label="Bam file" />
+        <param name="ref_file" type="data" format="fasta" optional="false" label="Reference file" />
+        <param name="var_file" type="data" format="vcf" optional="false" label="Variants file" />
+        <param name="input_genes" type="data" format="bed" optional="false" label="Gene file" />
+        <param name="mutation_db" type="data" format="tsv" optional="true" label="Mutation DB" help="Not required. Defaults to HIV mutation database." />
+        <param name="min_freq" type="float" optional="true" min="0" max="1" label="Minimum frequency" value="0.01" help="The minimum required frequency. Defaults to 0.01." />
+    </inputs>
+    <outputs>
+        <data format="vcf" name="output" from_work_dir="output.vcf" />
+    </outputs>
+    <tests>
+        <test>
+            <param name="input_bam" value="align.bam" />
+            <param name="ref_file" value="hxb2_pol.fas" />
+            <param name="var_file" value="nt_variants.vcf" />
+            <param name="input_genes" ftype="bed" value="hxb2_pol.bed" />
+            <param name="min_freq" value="0.01" />
+            <output name="output" >
+                <assert_contents>
+                    <has_text_matching expression="#CHROM\sGENE\sTYPE\sWILDTYPE\sPOS\sMUTANT\sFILTER\sMUTANT_FREQ\sCOVERAGE\sINFO"/>
+                    <has_text_matching expression="hxb2_pol\sRT\smutation\sK\s103\sN\sPASS\s0.0779\s154\sWC=aaa;MC=aaC;MCF=0.0779;CAT=.;SRVL=." />
+                    <has_text_matching expression="hxb2_pol\sIN\smutation\sG\s70\sE\sPASS\s0.0103\s2422\sWC=gga;MC=gAa;MCF=0.0103;CAT=.;SRVL=." />
+                    <has_text_matching expression="hxb2_pol\sIN\smutation\sE\s96\sG\sPASS\s0.0163\s1959\sWC=gaa;MC=gGa;MCF=0.0163;CAT=.;SRVL=." />
+                    <has_text_matching expression="hxb2_pol\sIN\smutation\sE\s96\sK\sPASS\s0.0148\s1959\sWC=gaa;MC=Aaa;MCF=0.0148;CAT=.;SRVL=." />
+                    <has_text_matching expression="hxb2_pol\sIN\smutation\sG\s189\sR\sPASS\s0.0234\s214\sWC=ggg;MC=Agg;MCF=0.0234;CAT=.;SRVL=." />
+                    <has_text_matching expression="hxb2_pol\sIN\smutation\sG\s192\sW\sPASS\s0.0159\s314\sWC=ggg;MC=Tgg;MCF=0.0159;CAT=.;SRVL=." />
+                </assert_contents>
+            </output>
+        </test>
+    </tests>
+    <help><![CDATA[
+
+Amino Acid Variants
+===================
+
+Identifies amino acid mutations.
+
+    ]]></help>
+    <citations>
+    </citations>
+</tool>