MultiplexCrisprDOE
Tool
Method: BioCCP
Description: BioCCP-based approaches for computing the minimal plant library size that guarantees full combinatorial coverage (and other relevant statistics)
Mode: BioCCP_γx3
Mode description: Computes the expected coverage of all triple combinations of gene knockouts (E[γx,3]) for an experiment with given plant library size using BioCCP
Variables
10 rows × 2 columns
| Argument | Value | |
|---|---|---|
| Any | Any | |
| 1 | # of target genes in the experiment | 20 |
| 2 | # of gRNAs designed per target gene | 6 |
| 3 | # of gRNAs / combi gRNA/Cas construct | 3 |
| 4 | Total number of gRNAs | 120 |
| 5 | Relative frequencies for all gRNAs | example_data.xlsx |
| 6 | Genome editing efficiencies for all gRNAs | example_data.xlsx |
| 7 | Global knockout efficiency | 0.8 |
| 8 | Step size | 5 |
| 9 | Maximum Plant library size | 6000 |
| 10 | Plant library size | 0 |
At a given number of plants, what is the expected coverage of pairwise gene knockout combinations? N_95: 6000
