Method: simulation
Description: simulation-based approaches for computing the minimal plant library size that guarantees full combinatorial coverage (and other relevant statistics)
Mode: simulateNx2countKOs
Mode description: Counts of the number of knockouts per plant in the experiment
8 rows × 2 columns
Argument | Value | |
---|---|---|
Any | Any | |
1 | # of target genes in the experiment | 20 |
2 | # of gRNAs designed per target gene | 6 |
3 | # of gRNAs / combi gRNA/Cas construct | 2 |
4 | Total number of gRNAs | 120 |
5 | Relative frequencies for all gRNAs | example_data.xlsx |
6 | Genome editing efficiencies for all gRNAs | example_data.xlsx |
7 | Global knockout efficiency | 0.8 |
8 | # of simulated experiments | 10 |
Output written to: countKOs.xlsx