MultiplexCrisprDOE

Tool

  • Method: simulation

  • Description: simulation-based approaches for computing the minimal plant library size that guarantees full combinatorial coverage (and other relevant statistics)

  • Mode: simulateNx2countKOs

  • Mode description: Counts of the number of knockouts per plant in the experiment

Variables

8 rows × 2 columns

ArgumentValue
AnyAny
1# of target genes in the experiment20
2# of gRNAs designed per target gene6
3# of gRNAs / combi gRNA/Cas construct2
4Total number of gRNAs120
5Relative frequencies for all gRNAsexample_data.xlsx
6Genome editing efficiencies for all gRNAsexample_data.xlsx
7Global knockout efficiency0.8
8# of simulated experiments10
Output written to:
countKOs.xlsx