MultiplexCrisprDOE

Tool

  • Method: BioCCP

  • Description: BioCCP-based approaches for computing the minimal plant library size that guarantees full combinatorial coverage (and other relevant statistics)

  • Mode: BioCCP_γx3

  • Mode description: Computes the expected coverage of all triple combinations of gene knockouts (E[γx,3]) for an experiment with given plant library size using BioCCP

Variables

10 rows × 2 columns

ArgumentValue
AnyAny
1# of target genes in the experiment20
2# of gRNAs designed per target gene6
3# of gRNAs / combi gRNA/Cas construct3
4Total number of gRNAs120
5Relative frequencies for all gRNAsexample_data.xlsx
6Genome editing efficiencies for all gRNAsexample_data.xlsx
7Global knockout efficiency0.8
8Step size5
9Maximum Plant library size6000
10Plant library size0
At a given number of plants, what is the expected coverage of pairwise gene
 knockout combinations?
N_95: 6000