Method: BioCCP
Description: BioCCP-based approaches for computing the minimal plant library size that guarantees full combinatorial coverage (and other relevant statistics)
Mode: BioCCP_γx2
Mode description: Computes the expected coverage of all pairwise combinations of gene knockouts (E[γx,2]) for an experiment with given plant library size using BioCCP
10 rows × 2 columns
Argument | Value | |
---|---|---|
Any | Any | |
1 | # of target genes in the experiment | 20 |
2 | # of gRNAs designed per target gene | 6 |
3 | # of gRNAs / combi gRNA/Cas construct | 2 |
4 | Total number of gRNAs | 120 |
5 | Relative frequencies for all gRNAs | ./test-data/example_data.xlsx |
6 | Genome editing efficiencies for all gRNAs | ./test-data/example_data.xlsx |
7 | Global knockout efficiency | 0.8 |
8 | Step size | 5 |
9 | Maximum Plant library size | 6000 |
10 | Plant library size | 0 |
At a given number of plants, what is the expected coverage of pairwise gene knockout combinations? N_95: 1315