Mercurial > repos > pavlo-lutsik > rnbeads
diff rnbeads.xml @ 11:ce411254e4a9 draft
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author | pavlo-lutsik |
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date | Sat, 20 Apr 2013 07:14:22 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/rnbeads.xml Sat Apr 20 07:14:22 2013 -0400 @@ -0,0 +1,502 @@ +<?xml version="0.9"?> +<tool id="rnbeads" name="RnBeads" version="0.9"> + <description>Performs RnBeads analysis for the selected set of </description> + <requirements> + <requirement type="set_environment" version="0.9">R_SCRIPTS_PATH</requirement> + <requirement type="package" version="3.0.0">R</requirement> + </requirements> + <command interpreter="bash">rnbeads_galaxy_wrapper.sh + #if str( $inputDataSelector.dataType ) == "idat" + --data-type="idat.dir" + --pheno="$inputDataSelector.sampleAnnotations" + #if str( $idatSelector.idatSelector.idatSource ) == "history" + --idat-files ==" + #for $input_files in $inputDataSelector.input_series: + ${input_files.input_files}\t + #end for + " + #else: + --idat-files == "$idatRepo" + #end if + #end if + #if str( $inputDataSelector.dataType ) == "gsreport" + --data-type="GS.report" + --gs-report="$inputDataSelector.gsReportFile" + #end if + #if str( $inputDataSelector.dataType ) == "geo" + --data-type="GEO" + --geo-series=$inputDataSelector.geoSeries + #end if + #if str( $inputDataSelector.dataType ) == "tables" + --data-type="data.files" + --pheno="$inputDataSelector.sampleAnnotations" + #end if + #if str( $inputDataSelector.dataType ) == "tables" + --data-type="data.files" + --pheno="$inputDataSelector.sampleAnnotations" + --betas="$inputDataSelector.betaTable" + --pvals="$inputDataSelector.pvalTable" + #end if + #if str( $inputDataSelector.dataType ) == "bed" + --data-type="bed.dir" + --pheno="$inputDataSelector.sampleAnnotations" + --beds ==" + #for $input_files in $inputDataSelector.input_series: + ${input_files.input_files}\t + #end for + #end if + --report-dir="$html_file.extra_files_path" + --output-file="$html_file" + #if str( $options.optionSet ) == "full" + #if str( $options.analysisName ) != "" + --analysis-name="$options.analysisName" + #end if + #if str( $options.logging ) == "True" + --logging + #end if + #if str( $options.email ) != "" + --email="$options.email" + #end if + #if str( $options.assembly ) != "" + --assembly="$options.assembly" + #end if + #if str( $options.analyzeSites ) == "True" + --analyze-sites + #end if + #if str( $options.regionTypes ) != "" + --region-types="$options.regionTypes" + #end if + #if str( $options.identifiersColumn ) != "" + --identifiers-column="$options.identifiersColumn" + #end if + #if str( $options.pointsCategory ) != "" + --points-category="$options.pointsCategory" + #end if + #if str( $options.colorsCategory ) != "" + --colors-category="$options.colorsCategory" + #end if + #if str( $options.colorsGradient ) != "" + --colors-gradient="$options.colorsGradient" + #end if + #if str( $options.minGroupSize ) != "" + --min-group-size="$options.minGroupSize" + #end if + #if str( $options.maxGroupCount ) != "" + --max-group-count="$options.maxGroupCount" + #end if + #if str( $options.gzLargeFiles ) == "True" + --gz-large-files + #end if + #if str( $options.strandSpecific ) == "True" + --strand-specific + #end if + #if str( $options.replicateIdColumn ) != "" + --replicate-id-column="$options.replicateIdColumn" + #end if + #if str( $options.loadingNormalization ) == "True" + --loading-normalization + #end if + #if str( $options.loadingDefaultDataType ) != "" + --loading-default-data-type="$options.loadingDefaultDataType" + #end if + #if str( $options.loadingTableSeparator ) != "" + --loading-table-separator="$options.loadingTableSeparator" + #end if + #if str( $options.loadingBedStyle ) != "" + --loading-bed-style="$options.loadingBedStyle" + #end if + #if str( $options.loadingBedColumns ) != "" + --loading-bed-columns="$options.loadingBedColumns" + #end if + #if str( $options.loadingBedFrameShift ) != "" + --loading-bed-frame-shift="$options.loadingBedFrameShift" + #end if + #if str( $options.normalizationMethod ) != "" + --normalization-method="$options.normalizationMethod" + #end if + #if str( $options.normalizationSubtractBackground ) == "True" + --normalization-subtract-background + #end if + #if str( $options.qc ) == "True" + --qc + #end if + #if str( $options.qcBoxplots ) == "True" + --qc-boxplots + #end if + #if str( $options.qcBarplots ) == "True" + --qc-barplots + #end if + #if str( $options.qcNegativeBoxplot ) == "True" + --qc-negative-boxplot + #end if + #if str( $options.qcSnpHeatmap ) == "True" + --qc-snp-heatmap + #end if + #if str( $options.qcSnpBoxplot ) == "True" + --qc-snp-boxplot + #end if + #if str( $options.qcSnpBarplot ) == "True" + --qc-snp-barplot + #end if + #if str( $options.qcSampleBatchSize ) != "" + --qc-sample-batch-size="$options.qcSampleBatchSize" + #end if + #if str( $options.filteringContextRemoval ) != "" + --filtering-context-removal="$options.filteringContextRemoval" + #end if + #if str( $options.filteringSnp ) == "True" + --filtering-snp + #end if + #if str( $options.filteringSnpFrequency ) != "" + --filtering-snp-frequency="$options.filteringSnpFrequency" + #end if + #if str( $options.filteringSnpAccepted ) != "" + --filtering-snp-accepted="$options.filteringSnpAccepted" + #end if + #if str( $options.filteringSexChromosomesRemoval ) == "True" + --filtering-sex-chromosomes-removal + #end if + #if str( $options.filteringMissingValueQuantile ) != "" + --filtering-missing-value-quantile="$options.filteringMissingValueQuantile" + #end if + #if str( $options.filteringCoverageThreshold ) != "" + --filtering-coverage-threshold="$options.filteringCoverageThreshold" + #end if + #if str( $options.filteringLowCoverageMasking ) == "True" + --filtering-low-coverage-masking + #end if + #if str( $options.filteringHighCoverageOutliers ) == "True" + --filtering-high-coverage-outliers + #end if + #if str( $options.filteringGreedycut ) == "True" + --filtering-greedycut + #end if + #if str( $options.filteringGreedycutPvalueThreshold ) != "" + --filtering-greedycut-pvalue-threshold="$options.filteringGreedycutPvalueThreshold" + #end if + #if str( $options.filteringGreedycutRcTies ) != "" + --filtering-greedycut-rc-ties="$options.filteringGreedycutRcTies" + #end if + #if str( $options.filteringDeviationThreshold ) != "" + --filtering-deviation-threshold="$options.filteringDeviationThreshold" + #end if + #if str( $options.batch ) == "True" + --batch + #end if + #if str( $options.batchDreductionColumns ) != "" + --batch-dreduction-columns="$options.batchDreductionColumns" + #end if + #if str( $options.batchPrincipalComponents ) != "" + --batch-principal-components="$options.batchPrincipalComponents" + #end if + #if str( $options.batchCorrelationColumns ) != "" + --batch-correlation-columns="$options.batchCorrelationColumns" + #end if + #if str( $options.batchCorrelationPvalueThreshold ) != "" + --batch-correlation-pvalue-threshold="$options.batchCorrelationPvalueThreshold" + #end if + #if str( $options.batchCorrelationPermutations ) != "" + --batch-correlation-permutations="$options.batchCorrelationPermutations" + #end if + #if str( $options.batchCorrelationQc ) == "True" + --batch-correlation-qc + #end if + #if str( $options.profiles ) == "True" + --profiles + #end if + #if str( $options.profilesBetaDistribution ) == "True" + --profiles-beta-distribution + #end if + #if str( $options.profilesIntersample ) == "True" + --profiles-intersample + #end if + #if str( $options.profilesDeviationPlots ) == "True" + --profiles-deviation-plots + #end if + #if str( $options.profilesColumns ) != "" + --profiles-columns="$options.profilesColumns" + #end if + #if str( $options.profilesClustering ) == "True" + --profiles-clustering + #end if + #if str( $options.profilesClusteringTopProbes ) != "" + --profiles-clustering-top-probes="$options.profilesClusteringTopProbes" + #end if + #if str( $options.regionProfilesTypes ) != "" + --region-profiles-types="$options.regionProfilesTypes" + #end if + #if str( $options.differential ) == "True" + --differential + #end if + #if str( $options.differentialPermutations ) != "" + --differential-permutations="$options.differentialPermutations" + #end if + #if str( $options.differentialComparisonColumns ) != "" + --differential-comparison-columns="$options.differentialComparisonColumns" + #end if + #if str( $options.differentialEnrichment ) == "True" + --differential-enrichment + #end if + #if str( $options.exportToUcsc ) != "" + --export-to-ucsc="$options.exportToUcsc" + #end if + #if str( $options.exportToBed ) == "True" + --export-to-bed + #end if + #if str( $options.exportToCsv ) == "True" + --export-to-csv + #end if + #if str( $options.exportTypes ) != "" + --export-types="$options.exportTypes" + #end if + #if str( $options.colors3Gradient ) != "" + --colors-3-gradient="$options.colors3Gradient" + #end if + #if str( $options.loggingMemory ) == "True" + --logging-memory + #end if + #if str( $options.usePstoimg ) == "True" + --use-pstoimg + #end if + #end if + </command> + <inputs> + <conditional name="inputDataSelector"> + <param name="dataType" type="select" label="Data Type"> + <option value="tables">Tabular data</option> + <option value="idats">IDAT files</option> + <option value="gsreport">GenomeStudio report</option> + <option value="geo">Gene Expression Omnibus series</option> + <option value="bed">BED files</option> + </param> + <when value="tables"> + <param name="sampleAnnotations" type="data" format="tabular" label="Sample Annotation File"/> + <param name="betaTable" type="data" format="tabular" label="Table with beta-values"/> + <param name="pvalTable" type="data" format="tabular" label="Table with detection p\-values"/> + </when> + <when value="idats"> + <param name="sampleAnnotations" type="data" format="tabular" label="Sample Annotation File"/> + <conditional name="idatSelector"> + <param name="idatSource" type="select" label="IDAT source"> + <option value="history">Select from history</option> + <option value="repo">Repository on the server</option> + </param> + <when value="repo"> + <param name="idatRepo" type="select" label="Select an IDAT repository"> + <options from_data_table="rnbeads_repos"> + <filter type="sort_by" column="2" /> + <validator type="no_options" message="No repositories are available" /> + </options> + </param> + </when> + <when value="history"> + <repeat min="1" name="idatSeries" title="Input IDAT file"> + <param name="idatFiles" type="data" format="idat" label="IDAT file"/> + </repeat> + </when> + </conditional> + </when> + <when value="gsreport"> + <param name="gsReportFile" type="data" format="tabular" label="GenomeStudio report" /> + </when> + <when value="geo"> + <param name="geoSeries" type="text" label="GEO series" /> + </when> + <when value="bed"> + <param name="sampleAnnotations" type="data" format="tabular" label="Sample Annotation File" /> + <repeat min="1" name="bedSeries" title="Input BED file"> + <param name="idatFiles" type="data" format="idat"/> + </repeat> + </when> + </conditional> + <conditional name="options"> + <param name="optionSet" type="select" label="Options Set"> + <option value="default">Default options</option> + <option value="full">Full option set</option> + </param> + <when value="default" /> + <when value="full"> + <param name="analysisName" type="text" label="analysis.name, character" value=""/> + <param name="logging" type="select" label="logging, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="email" type="text" label="email, character" value=""/> + <param name="assembly" type="text" label="assembly, character" value="hg19"/> + <param name="analyzeSites" type="select" label="analyze.sites, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="regionTypes" type="text" label="region.types, character vector" value="tiling,genes,promoters,cpgislands"/> + <param name="identifiersColumn" type="text" label="identifiers.column, character" value=""/> + <param name="pointsCategory" type="text" label="points.category, integer vector" value="16,17,3,15,4,7,8"/> + <param name="colorsCategory" type="text" label="colors.category, character vector" value="#1B9E77,#D95F02,#7570B3,#E7298A,#66A61E,#E6AB02,#A6761D,#666666"/> + <param name="colorsGradient" type="text" label="colors.gradient, character vector" value="#132B43,#56B1F7"/> + <param name="minGroupSize" type="text" label="min.group.size, integer" value="2"/> + <param name="maxGroupCount" type="text" label="max.group.count, integer" value="8"/> + <param name="gzLargeFiles" type="select" label="gz.large.files, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="strandSpecific" type="select" label="strand.specific, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="replicateIdColumn" type="text" label="replicate.id.column, character" value=""/> + <param name="loadingNormalization" type="select" label="loading.normalization, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="loadingDefaultDataType" type="text" label="loading.default.data.type, character" value="idat.dir"/> + <param name="loadingTableSeparator" type="text" label="loading.table.separator, character" value=","/> + <param name="loadingBedStyle" type="text" label="loading.bed.style, character" value="BisSNP"/> + <param name="loadingBedColumns" type="text" label="loading.bed.columns, integer vector" value="1,2,3,6,4,5"/> + <param name="loadingBedFrameShift" type="text" label="loading.bed.frame.shift, integer" value="1"/> + <param name="normalizationMethod" type="text" label="normalization.method, character" value="illumina"/> + <param name="normalizationSubtractBackground" type="select" label="normalization.subtract.background, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="qc" type="select" label="qc, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="qcBoxplots" type="select" label="qc.boxplots, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="qcBarplots" type="select" label="qc.barplots, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="qcNegativeBoxplot" type="select" label="qc.negative.boxplot, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="qcSnpHeatmap" type="select" label="qc.snp.heatmap, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="qcSnpBoxplot" type="select" label="qc.snp.boxplot, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="qcSnpBarplot" type="select" label="qc.snp.barplot, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="qcSampleBatchSize" type="text" label="qc.sample.batch.size, integer" value="50"/> + <param name="filteringContextRemoval" type="text" label="filtering.context.removal, character vector" value="CC,CAG,CAH,CTG,CTH,Other"/> + <param name="filteringSnp" type="select" label="filtering.snp, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="filteringSnpFrequency" type="text" label="filtering.snp.frequency, double" value="0.01"/> + <param name="filteringSnpAccepted" type="text" label="filtering.snp.accepted, integer" value="2"/> + <param name="filteringSexChromosomesRemoval" type="select" label="filtering.sex.chromosomes.removal, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="filteringMissingValueQuantile" type="text" label="filtering.missing.value.quantile, double" value="1"/> + <param name="filteringCoverageThreshold" type="text" label="filtering.coverage.threshold, integer" value="5"/> + <param name="filteringLowCoverageMasking" type="select" label="filtering.low.coverage.masking, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="filteringHighCoverageOutliers" type="select" label="filtering.high.coverage.outliers, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="filteringGreedycut" type="select" label="filtering.greedycut, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="filteringGreedycutPvalueThreshold" type="text" label="filtering.greedycut.pvalue.threshold, double" value="0.05"/> + <param name="filteringGreedycutRcTies" type="text" label="filtering.greedycut.rc.ties, character" value="row"/> + <param name="filteringDeviationThreshold" type="text" label="filtering.deviation.threshold, double" value="0"/> + <param name="batch" type="select" label="batch, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="batchDreductionColumns" type="text" label="batch.dreduction.columns, integer vector" value=""/> + <param name="batchPrincipalComponents" type="text" label="batch.principal.components, integer" value="8"/> + <param name="batchCorrelationColumns" type="text" label="batch.correlation.columns, integer vector" value=""/> + <param name="batchCorrelationPvalueThreshold" type="text" label="batch.correlation.pvalue.threshold, double" value="0.01"/> + <param name="batchCorrelationPermutations" type="text" label="batch.correlation.permutations, integer" value="10000"/> + <param name="batchCorrelationQc" type="select" label="batch.correlation.qc, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="profiles" type="select" label="profiles, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="profilesBetaDistribution" type="select" label="profiles.beta.distribution, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="profilesIntersample" type="select" label="profiles.intersample, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="profilesDeviationPlots" type="select" label="profiles.deviation.plots, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="profilesColumns" type="text" label="profiles.columns, integer vector" value=""/> + <param name="profilesClustering" type="select" label="profiles.clustering, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="profilesClusteringTopProbes" type="text" label="profiles.clustering.top.probes, integer vector" value="1000"/> + <param name="regionProfilesTypes" type="text" label="region.profiles.types, character vector" value="genes,promoters,cpgislands"/> + <param name="differential" type="select" label="differential, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="differentialPermutations" type="text" label="differential.permutations, integer" value="0"/> + <param name="differentialComparisonColumns" type="text" label="differential.comparison.columns, integer vector" value=""/> + <param name="differentialEnrichment" type="select" label="differential.enrichment, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="exportToUcsc" type="text" label="export.to.ucsc, character vector" value="bigBed,bigWig"/> + <param name="exportToBed" type="select" label="export.to.bed, logical" value="1"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="exportToCsv" type="select" label="export.to.csv, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="exportTypes" type="text" label="export.types, character vector" value="sites"/> + <param name="colors3Gradient" type="text" label="colors.3.gradient, character vector" value="#832424,#FFFFFF,#3A3A98"/> + <param name="loggingMemory" type="select" label="logging.memory, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + <param name="usePstoimg" type="select" label="use.pstoimg, logical" value="0"> + <option value="True">True</option> + <option value="False">False</option> + </param> + </when> + </conditional> + </inputs> + + <outputs> + <data format="html" name="html_file" label="index.html" /> + </outputs> + + <!-- + <tests> + <test> + <param name="input" value="fa_gc_content_input.fa"/> + <output name="out_file1" file="fa_gc_content_output.txt"/> + </test> + </tests> --> + + <help> + RnBeads is an R-package for comprehensive analysis of Illumina Infinium HumanMethylation450 BeadChip data. It extends previous approaches for this type of data analysis by high throughput capabilities, as well as presenting results in a comprehensive, highly interpretable fashion. + </help> + +</tool> \ No newline at end of file