Mercurial > repos > pavlo-lutsik > rnbeads
view RnBeadsGalaxy.R @ 45:6b0981ab063e draft default tip
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author | pavlo-lutsik |
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date | Thu, 14 Aug 2014 14:56:18 -0400 |
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#msg.file<-file("RnBeads.messages.out", open="w") #sink(file=msg.file) ## add the RnBeads dependencies if we are on a cloud share-instance if("Rsitelibrary" %in% list.files("/mnt")){ .libPaths("/mnt/galaxy/Rsitelibrary") } if(!'wordcloud' %in% rownames(installed.packages())){ install.packages('wordcloud',repos='http://cran.us.r-project.org') } suppressWarnings(suppressPackageStartupMessages(library(RnBeads))) suppressWarnings(suppressPackageStartupMessages(library(getopt))) fname <- system.file(file.path("extdata", "options.txt"), package = "RnBeads") table.options <- read.delim(fname, quote = "", stringsAsFactors = FALSE) rownames(table.options) <- table.options[, "Name"] all.opts<-table.options$Name #all.opts<-names(rnb.options()) #opt.class<-RnBeads:::OPTION.TYPES[-28] opt.class<-table.options$Type names(opt.class)<-all.opts #all.opts<-names(opt.class) #all.opts<-paste("--", all.opts, sep="") #all.opts<-gsub("\\.([a-z])", "\\U\\1", all.opts, perl=TRUE) all.opts<-gsub("\\.","-", all.opts) #opt.class<-sapply(rnb.options(), class) rnb.opt.spec<-data.frame( Long=all.opts, Short=as.character(1:length(all.opts)), Mask=c(1,2)[as.integer((opt.class=="logical"))+1], Type=opt.class) ### automated xml file preparation #xml.strings<-apply(rnb.opt.spec,1, function(row){ # # opt.lab<-gsub("-", ".", row[1]) # opt.def.val<-rnb.getOption(opt.lab) # #print(opt.def.val) # opt.name<-gsub("-([0-9a-z])", "\\U\\1", row[1], perl=TRUE) # tf.opt<-"\t\t\t<option value=\"True\">True</option>\n\t\t\t<option value=\"False\">False</option>" # opt.lab<-paste(opt.lab, gsub("\\."," ", row[4]), sep=", ") # if(row[4]=="logical"){ # opt.type<-'select' # if(!is.null(opt.def.val) && opt.def.val) # opt.def.val<-"1" else # opt.def.val<-"0" # string<-sprintf("\t\t<param name=\"%s\" type=\"%s\" label=\"%s\" value=\"%s\">\n%s\n\t\t</param>\n", opt.name, opt.type, opt.lab, opt.def.val, tf.opt) # }else{ # opt.type<-'text' # if(length(opt.def.val)<1L){ # opt.def.val<-"" # }else{ # if(!is.null(opt.def.val) && opt.def.val!="") # opt.def.val<-paste(opt.def.val, collapse=",") else # opt.def.val<-"" # } # string<-sprintf("\t\t<param name=\"%s\" type=\"%s\" label=\"%s\" value=\"%s\"/>\n", opt.name, opt.type, opt.lab, opt.def.val) # } # string # }) ##cat(xml.strings, sep="", file="automated.settings.xml.txt") # #opt.def.strings<-apply(rnb.opt.spec,1, function(row){ # # opt.name<-gsub("-([0-9a-z])", "\\U\\1", row[1], perl=TRUE) # opt.long<-row[1] # opt.short<-row[2] # # if(row[4]=="logical"){ # def.string<-sprintf("#if str( $options.%s ) == \"True\"\n\t--%s\n#end if\n", opt.name, opt.long) # }else{ # def.string<-sprintf("#if str( $options.%s ) != \"\"\n\t--%s=\"$options.%s\" \n#end if\n", opt.name, opt.long, opt.name) # } # def.string # # }) #cat(opt.def.strings, sep="", file="automated.option.assignments.txt") rnb.opt.spec$Type<-gsub("\\.vector", "", rnb.opt.spec$Type) rnb.opt.spec$Type<-gsub("numeric", "double", rnb.opt.spec$Type) rnb.opt.spec<-rbind(data.frame( Long=c("data-type", "pheno", "idat-dir","idat-files","bed-files", "gs-report", "geo-series", "betas", "pvals","output-file", "report-dir"), Short=c("d","s","a","i","f","g","e","b","p","r","o"), Mask=c(1,2,2,2,2,2,2,2,2,1,1), Type=c("character","character","character","character","character","character","character","character","character","character", "character")), rnb.opt.spec) opts<-getopt(as.matrix(rnb.opt.spec)) #opts<-getopt(as.matrix(rnb.opt.spec), opt=list("--data-type=idats","--report-dir=dir", "--idats=file1\tfile2")) print(opts) if(opts[["data-type"]]=="idatDir"){ data.source<-list() data.type<-"idat.dir" data.source[["idat.dir"]]<-opts[["idat-dir"]] data.source[["sample.sheet"]]<-opts[["pheno"]] }else if(opts[["data-type"]]=="idatFiles"){ data.type<-"idat.dir" file.string<-gsub(" ","", opts[["idat-files"]]) files<-strsplit(file.string, ",")[[1]] files<-files[files!=""] bed.dir<-sprintf("%s_beds",opts[["report-dir"]]) dir.create(bed.dir) file.copy(files, bed.dir) for(dat.file in list.files(bed.dir, full.names = TRUE)){ file.rename(dat.file, gsub("\\.dat", ".bed", dat.file)) } data.source<-list() data.source[["bed.dir"]]<-bed.dir data.source[["sample.sheet"]]<-opts[["pheno"]] }else if(opts[["data-type"]]=="GS.report"){ data.type<-"GS.report" data.source<-opts[["gs-report"]] }else if(opts[["data-type"]]=="GEO"){ data.type<-"GEO" data.source<-opts[["geo-series"]] }else if(opts[["data-type"]]=="data.files"){ data.type<-"GEO" data.source<-opts[["geo-series"]] }else if(opts[["data-type"]]=="data.files"){ data.type<-"data.files" data.source<-c(opts[["pheno"]], opts[["betas"]]) if(!is.null(opts[["pvals"]])) data.source<-c(data.source, opts[["pvals"]]) }else if(opts[["data-type"]]=="bed.dir"){ data.type<-"bed.dir" file.string<-gsub(" ","", opts[["bed-files"]]) files<-strsplit(file.string, ",")[[1]] files<-files[files!=""] bed.dir<-sprintf("%s_beds",opts[["report-dir"]]) dir.create(bed.dir) file.copy(files, bed.dir) for(dat.file in list.files(bed.dir, full.names = TRUE)){ file.rename(dat.file, gsub("\\.dat", ".bed", dat.file)) } data.source<-list() data.source[["bed.dir"]]<-bed.dir logger.start(fname="NA") sample.sheet<-read.sample.annotation(opts[["pheno"]]) logger.close() if(length(files) < nrow(sample.sheet)) stop("Not all bed files are present") cn<-colnames(sample.sheet) dat.files<-sapply(strsplit(files, "\\/"), function(el) el[length(el)]) sample.sheet<-cbind(sample.sheet, gsub("\\.dat", ".bed", dat.files)) colnames(sample.sheet)<-c(cn, "BED_files") data.source[["sample.sheet"]]<-sample.sheet } if("logging" %in% names(opts)){ # TODO create a cleaner way of checking whether the full options set was supplied dump<-sapply(names(opt.class), function(on){ getoptname<-gsub("-", "\\.","-",on) if(getoptname %in% names(opts)){ if(opt.class[on]=="logical"){ ov<-TRUE }else if(opt.class %in% c("character","character.vector")){ ov<-opts[[getoptname]] ov<-gsub("\"", "", ov) if(opt.class=="character.vector"){ ov<-as.character(strsplit(ov,",")) } }else if(opt.class %in% c("integer","numeric","integer.vector","numeric.vector")){ ov<-opts[[getoptname]] ov<-gsub("\"", "", ov) if(opt.class %in% c("integer.vector","numeric.vector")){ ov<-as.character(strsplit(ov,",")) } } eval(parse(text=sprintf("rnb.options(%s=ov)",on))) } }) logical.opts<-names(opt.class[opt.class=="logical"]) logical.opts.false<-logical.opts[!logical.opts %in% gsub("-",".",names(opts))] dump<-sapply(logical.opts.false, function(on){ eval(parse(text=sprintf("rnb.options(%s=FALSE)",on))) }) } print(rnb.options()) #report.out.dir<-sprintf("%s_rnbReport", tempdir()) report.out.dir<-opts[["report-dir"]] print("Starting RnBeads with the following inputs:") print(data.source) print(report.out.dir) print(data.type) rnb.run.analysis(data.source=data.source, dir.report=report.out.dir, data.type=data.type) #sink(file=NULL) #flush(msg.file) #close(msg.file)