# HG changeset patch
# User pavlo-lutsik
# Date 1373029721 14400
# Node ID 450c8ff1d11a76772733029a4a79627a98bd1b75
# Parent  12b2947c0905f11b40fdffef6e841d2d08f90720
Uploaded

diff -r 12b2947c0905 -r 450c8ff1d11a RnBeadsGalaxy.R
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/RnBeadsGalaxy.R	Fri Jul 05 09:08:41 2013 -0400
@@ -0,0 +1,198 @@
+#msg.file<-file("RnBeads.messages.out", open="w")
+#sink(file=msg.file)
+
+suppressWarnings(suppressPackageStartupMessages(library(RnBeads)))
+suppressWarnings(suppressPackageStartupMessages(library(getopt)))
+
+#all.opts<-names(rnb.options())
+opt.class<-RnBeads:::OPTION.TYPES[-28]
+all.opts<-names(opt.class)
+#all.opts<-paste("--", all.opts, sep="")
+#all.opts<-gsub("\\.([a-z])", "\\U\\1", all.opts, perl=TRUE)
+all.opts<-gsub("\\.","-", all.opts)
+#opt.class<-sapply(rnb.options(), class)
+
+rnb.opt.spec<-data.frame(
+		Long=all.opts, 
+		Short=as.character(1:length(all.opts)), 
+		Mask=c(1,2)[as.integer((opt.class=="logical"))+1], 
+		Type=opt.class)
+
+### automated xml file preparation
+xml.strings<-apply(rnb.opt.spec,1, function(row){
+			
+			opt.lab<-gsub("-", ".", row[1])
+			opt.def.val<-rnb.getOption(opt.lab)
+			opt.name<-gsub("-([0-9a-z])", "\\U\\1", row[1], perl=TRUE)
+			tf.opt<-"\t\t\t<option value=\"True\">True</option>\n\t\t\t<option value=\"False\">False</option>"
+			opt.lab<-paste(opt.lab, gsub("\\."," ", row[4]), sep=", ")
+			if(row[4]=="logical"){
+				opt.type<-'select'
+				if(!is.null(opt.def.val) && opt.def.val)
+					opt.def.val<-"1" else
+					opt.def.val<-"0"
+				string<-sprintf("\t\t<param name=\"%s\" type=\"%s\" label=\"%s\" value=\"%s\">\n%s\n\t\t</param>\n", opt.name, opt.type, opt.lab, opt.def.val, tf.opt)
+			}else{
+				opt.type<-'text'
+				if(!is.null(opt.def.val) && opt.def.val!="")
+					opt.def.val<-paste(opt.def.val, collapse=",") else
+					opt.def.val<-""
+				string<-sprintf("\t\t<param name=\"%s\" type=\"%s\"  label=\"%s\" value=\"%s\"/>\n", opt.name, opt.type, opt.lab, opt.def.val)
+			}
+			string
+		})
+
+cat(xml.strings, sep="", file="automated.settings.xml.txt")
+
+opt.def.strings<-apply(rnb.opt.spec,1, function(row){
+			
+			opt.name<-gsub("-([0-9a-z])", "\\U\\1", row[1], perl=TRUE)
+			opt.long<-row[1]
+			opt.short<-row[2]
+			
+			if(row[4]=="logical"){
+				def.string<-sprintf("#if str( $options.%s ) == \"True\"\n\t--%s\n#end if\n", opt.name, opt.long)		
+			}else{
+				def.string<-sprintf("#if str( $options.%s ) != \"\"\n\t--%s=\"$options.%s\" \n#end if\n", opt.name, opt.long, opt.name)
+			}
+			def.string
+			
+		})
+cat(opt.def.strings, sep="", file="automated.option.assignments.txt")
+
+
+rnb.opt.spec$Type<-gsub("\\.vector", "", rnb.opt.spec$Type)
+rnb.opt.spec$Type<-gsub("numeric", "double", rnb.opt.spec$Type)
+rnb.opt.spec<-rbind(data.frame(
+				Long=c("data-type", "pheno", "idat-dir","idat-files","bed-files", "gs-report", "geo-series", "betas", "pvals","output-file", "report-dir"),
+				Short=c("d","s","a","i","f","g","e","b","p","r","o"),
+				Mask=c(1,2,2,2,2,2,2,2,2,1,1),
+				Type=c("character","character","character","character","character","character","character","character","character","character", "character")),
+				rnb.opt.spec)
+		
+opts<-getopt(as.matrix(rnb.opt.spec))
+#opts<-getopt(as.matrix(rnb.opt.spec), opt=list("--data-type=idats","--report-dir=dir", "--idats=file1\tfile2"))
+print(opts)
+
+if(opts[["data-type"]]=="idatDir"){
+   
+	data.source<-list()
+	data.type<-"idat.dir"
+	data.source[["idat.dir"]]<-opts[["idat-dir"]]
+	data.source[["sample.sheet"]]<-opts[["pheno"]]
+   
+}else if(opts[["data-type"]]=="idatFiles"){
+	
+	data.type<-"idat.dir"
+	file.string<-gsub(" ","", opts[["idat-files"]])
+	files<-strsplit(file.string, ",")[[1]]
+	files<-files[files!=""]
+	bed.dir<-sprintf("%s_beds",opts[["report-dir"]])
+	dir.create(bed.dir)
+	file.copy(files, bed.dir)
+	for(dat.file in list.files(bed.dir, full.names = TRUE)){
+		file.rename(dat.file, gsub("\\.dat", ".bed", dat.file))
+	}
+	data.source<-list()
+	data.source[["bed.dir"]]<-bed.dir
+	data.source[["sample.sheet"]]<-opts[["pheno"]]
+	
+	
+}else if(opts[["data-type"]]=="GS.report"){
+
+	data.type<-"GS.report"
+	data.source<-opts[["gs-report"]]
+	
+}else if(opts[["data-type"]]=="GEO"){
+	
+	data.type<-"GEO"
+	data.source<-opts[["geo-series"]]
+	
+}else if(opts[["data-type"]]=="data.files"){
+	
+	data.type<-"GEO"
+	data.source<-opts[["geo-series"]]
+	
+}else if(opts[["data-type"]]=="data.files"){
+	
+	data.type<-"data.files"
+	data.source<-c(opts[["pheno"]], opts[["betas"]])
+	if(!is.null(opts[["pvals"]]))
+		data.source<-c(data.source, opts[["pvals"]])
+		
+}else if(opts[["data-type"]]=="bed.dir"){
+
+	data.type<-"bed.dir"
+	file.string<-gsub(" ","", opts[["bed-files"]])
+	files<-strsplit(file.string, ",")[[1]]
+	files<-files[files!=""]
+	bed.dir<-sprintf("%s_beds",opts[["report-dir"]])
+	dir.create(bed.dir)
+	file.copy(files, bed.dir)
+	for(dat.file in list.files(bed.dir, full.names = TRUE)){
+		file.rename(dat.file, gsub("\\.dat", ".bed", dat.file))
+	}
+	data.source<-list()
+	data.source[["bed.dir"]]<-bed.dir
+	
+	logger.start(fname="NA")
+	sample.sheet<-rnb.read.annotation(opts[["pheno"]])
+	logger.close()
+	if(length(files) < nrow(sample.sheet))
+		stop("Not all bed files are present")
+	
+	cn<-colnames(sample.sheet)
+	dat.files<-sapply(strsplit(files, "\\/"), function(el) el[length(el)])
+	sample.sheet<-cbind(sample.sheet, gsub("\\.dat", ".bed", dat.files))
+	colnames(sample.sheet)<-c(cn, "BED_files")
+	data.source[["sample.sheet"]]<-sample.sheet
+}
+
+if("logging" %in% names(opts)){ # TODO create a cleaner way of checking whether the full options set was supplied
+	
+	dump<-sapply(names(opt.class), function(on){
+		getoptname<-gsub("-", "\\.","-",on)
+		if(getoptname %in% names(opts)){
+			if(opt.class[on]=="logical"){
+				ov<-TRUE
+			}else if(opt.class %in% c("character","character.vector")){
+				ov<-opts[[getoptname]]
+				ov<-gsub("\"", "", ov)
+				if(opt.class=="character.vector"){
+					ov<-as.character(strsplit(ov,","))
+				}
+				
+			}else if(opt.class %in% c("integer","numeric","integer.vector","numeric.vector")){
+				ov<-opts[[getoptname]]
+				ov<-gsub("\"", "", ov)
+				if(opt.class %in% c("integer.vector","numeric.vector")){
+					ov<-as.character(strsplit(ov,","))
+				}
+			}
+			eval(parse(text=sprintf("rnb.options(%s=ov)",on)))
+		}
+	})
+	
+	logical.opts<-names(opt.class[opt.class=="logical"])
+	logical.opts.false<-logical.opts[!logical.opts %in% gsub("-",".",names(opts))]
+	
+	
+	dump<-sapply(logical.opts.false, function(on){
+				eval(parse(text=sprintf("rnb.options(%s=FALSE)",on)))	
+			})
+}
+
+print(rnb.options())
+
+#report.out.dir<-sprintf("%s_rnbReport", tempdir())
+report.out.dir<-opts[["report-dir"]]
+print("Starting RnBeads with the following inputs:")
+print(data.source)
+print(report.out.dir)
+print(data.type)
+rnb.run.analysis(data.source=data.source, dir.report=report.out.dir, data.type=data.type)
+
+
+#sink(file=NULL)
+#flush(msg.file)
+#close(msg.file)
\ No newline at end of file
diff -r 12b2947c0905 -r 450c8ff1d11a install.rnbeads.R
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/install.rnbeads.R	Fri Jul 05 09:08:41 2013 -0400
@@ -0,0 +1,1 @@
+source("http://rnbeads.mpi-inf.mpg.de/install.R")
\ No newline at end of file
diff -r 12b2947c0905 -r 450c8ff1d11a rnbeads.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/rnbeads.xml	Fri Jul 05 09:08:41 2013 -0400
@@ -0,0 +1,506 @@
+<?xml version="0.9"?>
+<tool id="rnbeads" name="RnBeads" version="0.9">
+  <description>Performs RnBeads analysis for the selected set of </description>
+  <requirements>
+    <requirement type="set_environment" version="1.0">R_SCRIPTS_PATH</requirement>
+    <requirement type="package" version="3.0.0">R</requirement>
+  </requirements>
+  <command interpreter="bash">rnbeads_galaxy_wrapper.sh
+  		--report-dir="$html_file.extra_files_path"
+      	--output-file="$html_file"
+      #if str( $inputDataSelector.dataType ) == "idats"  
+      	--pheno="$inputDataSelector.sampleAnnotations"
+      #end if
+      #if str( $inputDataSelector.dataType ) != "idats"
+      	#pass
+      #else if str( $inputDataSelector.idatSelector.idatSource ) == "history"
+      	--data-type="idatFiles"
+      	#set $idatList=""
+      	#for $input_file in $inputDataSelector.idatSelector.idatSeries:
+    		#set $idatList+=str( $input_file.idatFile )
+    		#set $idatList+=","				
+    	#end for
+    	--idat-files="$idatList"
+      #else:
+      		--data-type="idatDir"
+      		--idat-dir="$inputDataSelector.idatSelector.idatRepo.fields.path"
+      #end if
+      #if str( $inputDataSelector.dataType ) == "gsreport"
+      	--data-type="GS.report"
+      	--gs-report="$inputDataSelector.gsReportFile"
+      #end if
+      #if str( $inputDataSelector.dataType ) == "geo"
+      	--data-type="GEO"
+      	--geo-series=$inputDataSelector.geoSeries
+      #end if
+      #if str( $inputDataSelector.dataType ) == "tables"
+        --data-type="data.files"
+        --pheno="$inputDataSelector.sampleAnnotations"
+      #end if
+      #if str( $inputDataSelector.dataType ) == "tables"
+      	--data-type="data.files"
+      	--pheno="$inputDataSelector.sampleAnnotations"
+      	--betas="$inputDataSelector.betaTable"
+      	--pvals="$inputDataSelector.pvalTable"
+      #end if
+      #if str( $inputDataSelector.dataType ) == "bed"
+        --data-type="bed.dir"
+      	--pheno="$inputDataSelector.sampleAnnotations"
+        #set $bedList=""
+      	#for $input_file in $inputDataSelector.bedSeries:
+    		#set $bedList+=str( $input_file.bedFile )
+    		#set $bedList+=","				
+    	#end for
+    	--bed-files="$bedList"
+      #end if
+      #if str( $options.optionSet ) == "full"
+		#if str( $options.analysisName ) != ""
+			--analysis-name="$options.analysisName" 
+		#end if
+		#if str( $options.logging ) == "True"
+			--logging
+		#end if
+		#if str( $options.email ) != ""
+			--email="$options.email" 
+		#end if
+		#if str( $options.assembly ) != ""
+			--assembly="$options.assembly" 
+		#end if
+		#if str( $options.columnsPairing ) != ""
+			--columns-pairing="$options.columnsPairing" 
+		#end if
+		#if str( $options.analyzeSites ) == "True"
+			--analyze-sites
+		#end if
+		#if str( $options.regionTypes ) != ""
+			--region-types="$options.regionTypes" 
+		#end if
+		#if str( $options.identifiersColumn ) != ""
+			--identifiers-column="$options.identifiersColumn" 
+		#end if
+		#if str( $options.pointsCategory ) != ""
+			--points-category="$options.pointsCategory" 
+		#end if
+		#if str( $options.colorsCategory ) != ""
+			--colors-category="$options.colorsCategory" 
+		#end if
+		#if str( $options.colorsGradient ) != ""
+			--colors-gradient="$options.colorsGradient" 
+		#end if
+		#if str( $options.minGroupSize ) != ""
+			--min-group-size="$options.minGroupSize" 
+		#end if
+		#if str( $options.maxGroupCount ) != ""
+			--max-group-count="$options.maxGroupCount" 
+		#end if
+		#if str( $options.gzLargeFiles ) == "True"
+			--gz-large-files
+		#end if
+		#if str( $options.strandSpecific ) == "True"
+			--strand-specific
+		#end if
+		#if str( $options.replicateIdColumn ) != ""
+			--replicate-id-column="$options.replicateIdColumn" 
+		#end if
+		#if str( $options.loadingNormalization ) == "True"
+			--loading-normalization
+		#end if
+		#if str( $options.loadingDefaultDataType ) != ""
+			--loading-default-data-type="$options.loadingDefaultDataType" 
+		#end if
+		#if str( $options.loadingTableSeparator ) != ""
+			--loading-table-separator="$options.loadingTableSeparator" 
+		#end if
+		#if str( $options.loadingBedStyle ) != ""
+			--loading-bed-style="$options.loadingBedStyle" 
+		#end if
+		#if str( $options.loadingBedColumns ) != ""
+			--loading-bed-columns="$options.loadingBedColumns" 
+		#end if
+		#if str( $options.loadingBedFrameShift ) != ""
+			--loading-bed-frame-shift="$options.loadingBedFrameShift" 
+		#end if
+		#if str( $options.normalizationMethod ) != ""
+			--normalization-method="$options.normalizationMethod" 
+		#end if
+		#if str( $options.normalizationBackgroundMethod ) != ""
+			--normalization-background-method="$options.normalizationBackgroundMethod" 
+		#end if
+		#if str( $options.qc ) == "True"
+			--qc
+		#end if
+		#if str( $options.qcBoxplots ) == "True"
+			--qc-boxplots
+		#end if
+		#if str( $options.qcBarplots ) == "True"
+			--qc-barplots
+		#end if
+		#if str( $options.qcSnpHeatmap ) == "True"
+			--qc-snp-heatmap
+		#end if
+		#if str( $options.qcSnpHeatmap ) == "True"
+			--qc-snp-heatmap
+		#end if
+		#if str( $options.qcSnpBoxplot ) == "True"
+			--qc-snp-boxplot
+		#end if
+		#if str( $options.qcSnpBarplot ) == "True"
+			--qc-snp-barplot
+		#end if
+		#if str( $options.qcSampleBatchSize ) != ""
+			--qc-sample-batch-size="$options.qcSampleBatchSize" 
+		#end if
+		#if str( $options.filteringContextRemoval ) != ""
+			--filtering-context-removal="$options.filteringContextRemoval" 
+		#end if
+		#if str( $options.filteringSnp ) == "True"
+			--filtering-snp
+		#end if
+		#if str( $options.filteringSnpFrequency ) != ""
+			--filtering-snp-frequency="$options.filteringSnpFrequency" 
+		#end if
+		#if str( $options.filteringSnpAccepted ) != ""
+			--filtering-snp-accepted="$options.filteringSnpAccepted" 
+		#end if
+		#if str( $options.filteringSexChromosomesRemoval ) == "True"
+			--filtering-sex-chromosomes-removal
+		#end if
+		#if str( $options.filteringMissingValueQuantile ) != ""
+			--filtering-missing-value-quantile="$options.filteringMissingValueQuantile" 
+		#end if
+		#if str( $options.filteringCoverageThreshold ) != ""
+			--filtering-coverage-threshold="$options.filteringCoverageThreshold" 
+		#end if
+		#if str( $options.filteringLowCoverageMasking ) == "True"
+			--filtering-low-coverage-masking
+		#end if
+		#if str( $options.filteringHighCoverageOutliers ) == "True"
+			--filtering-high-coverage-outliers
+		#end if
+		#if str( $options.filteringGreedycut ) == "True"
+			--filtering-greedycut
+		#end if
+		#if str( $options.filteringGreedycutPvalueThreshold ) != ""
+			--filtering-greedycut-pvalue-threshold="$options.filteringGreedycutPvalueThreshold" 
+		#end if
+		#if str( $options.filteringGreedycutRcTies ) != ""
+			--filtering-greedycut-rc-ties="$options.filteringGreedycutRcTies" 
+		#end if
+		#if str( $options.filteringDeviationThreshold ) != ""
+			--filtering-deviation-threshold="$options.filteringDeviationThreshold" 
+		#end if
+		#if str( $options.batch ) == "True"
+			--batch
+		#end if
+		#if str( $options.batchDreductionColumns ) != ""
+			--batch-dreduction-columns="$options.batchDreductionColumns" 
+		#end if
+		#if str( $options.batchPrincipalComponents ) != ""
+			--batch-principal-components="$options.batchPrincipalComponents" 
+		#end if
+		#if str( $options.batchCorrelationColumns ) != ""
+			--batch-correlation-columns="$options.batchCorrelationColumns" 
+		#end if
+		#if str( $options.batchCorrelationPvalueThreshold ) != ""
+			--batch-correlation-pvalue-threshold="$options.batchCorrelationPvalueThreshold" 
+		#end if
+		#if str( $options.batchCorrelationPermutations ) != ""
+			--batch-correlation-permutations="$options.batchCorrelationPermutations" 
+		#end if
+		#if str( $options.batchCorrelationQc ) == "True"
+			--batch-correlation-qc
+		#end if
+		#if str( $options.profiles ) == "True"
+			--profiles
+		#end if
+		#if str( $options.profilesBetaDistribution ) == "True"
+			--profiles-beta-distribution
+		#end if
+		#if str( $options.profilesIntersample ) == "True"
+			--profiles-intersample
+		#end if
+		#if str( $options.profilesDeviationPlots ) == "True"
+			--profiles-deviation-plots
+		#end if
+		#if str( $options.profilesColumns ) != ""
+			--profiles-columns="$options.profilesColumns" 
+		#end if
+		#if str( $options.profilesClustering ) == "True"
+			--profiles-clustering
+		#end if
+		#if str( $options.profilesClusteringTopProbes ) != ""
+			--profiles-clustering-top-probes="$options.profilesClusteringTopProbes" 
+		#end if
+		#if str( $options.regionProfilesTypes ) != ""
+			--region-profiles-types="$options.regionProfilesTypes" 
+		#end if
+		#if str( $options.differential ) == "True"
+			--differential
+		#end if
+		#if str( $options.differentialPermutations ) != ""
+			--differential-permutations="$options.differentialPermutations" 
+		#end if
+		#if str( $options.differentialComparisonColumns ) != ""
+			--differential-comparison-columns="$options.differentialComparisonColumns" 
+		#end if
+		#if str( $options.differentialComparisonColumnsAllPairwise ) != ""
+			--differential-comparison-columns-all-pairwise="$options.differentialComparisonColumnsAllPairwise" 
+		#end if
+		#if str( $options.differentialEnrichment ) == "True"
+			--differential-enrichment
+		#end if
+		#if str( $options.exportToUcsc ) != ""
+			--export-to-ucsc="$options.exportToUcsc" 
+		#end if
+		#if str( $options.exportToBed ) == "True"
+			--export-to-bed
+		#end if
+		#if str( $options.exportToCsv ) == "True"
+			--export-to-csv
+		#end if
+		#if str( $options.exportTypes ) != ""
+			--export-types="$options.exportTypes" 
+		#end if
+		#if str( $options.colors3Gradient ) != ""
+			--colors-3-gradient="$options.colors3Gradient" 
+		#end if
+		#if str( $options.loggingMemory ) == "True"
+			--logging-memory
+		#end if
+      #end if
+  </command>
+  <inputs>
+    <conditional name="inputDataSelector">
+    	<param name="dataType" type="select" label="Data Type">
+    		<option value="tables">Tabular data</option>
+    		<option value="idats">IDAT files</option>
+    		<option value="gsreport">GenomeStudio report</option>
+    		<option value="geo">Gene Expression Omnibus series</option>		
+    		<option value="bed">BED files</option>
+    	</param>
+    	<when value="tables">
+    		<param name="sampleAnnotations" type="data" format="tabular" label="Sample Annotation File"/>
+    		<param name="betaTable" type="data" format="tabular" label="Table with beta-values"/> 	
+    		<param name="pvalTable" type="data" format="tabular" label="Table with detection p\-values"/>
+    	</when>
+    	<when value="idats">
+    		<param name="sampleAnnotations" type="data" format="tabular" label="Sample Annotation File"/>
+    		<conditional name="idatSelector">
+    			<param name="idatSource" type="select" label="IDAT source">
+    				<option value="history">Select from history</option>
+    				<option value="repo">Repository on the server</option>
+    			</param>
+    			<when value="repo">
+    				<param name="idatRepo" type="select" label="Select an IDAT repository">
+    					<options from_data_table="rnbeads_repos">
+            				<filter type="sort_by" column="2" />
+            				<validator type="no_options" message="No repositories are available" />
+          				</options>
+          			</param>
+    			</when>
+    			<when value="history">
+    				<repeat min="1" name="idatSeries" title="Input IDAT file">
+    					<param name="idatFile" type="data" format="idat" label="IDAT file"/>
+    				</repeat>
+    			</when>
+    		</conditional>
+    	</when>
+    	<when value="gsreport">
+    		<param name="gsReportFile" type="data" format="tabular" label="GenomeStudio report" />
+    	</when>
+    	<when value="geo">
+    		<param name="geoSeries" type="text" label="GEO series" />
+    	</when>
+    	<when value="bed">
+    		<param name="sampleAnnotations" type="data" format="tabular" label="Sample Annotation File" />
+   			<repeat min="1" name="bedSeries" title="Input BED file">
+    			<param name="bedFile" type="data" format="bed" label="BED file"/>
+    		</repeat>
+        </when>
+    </conditional>
+    <conditional name="options">
+    	<param name="optionSet" type="select" label="Options Set">
+    		<option value="default">Default options</option>
+    		<option value="full">Full option set</option>
+    	</param>
+    	<when value="default" />
+    	<when value="full">
+    		<param name="analysisName" type="text"  label="analysis.name, character" value=""/>
+			<param name="logging" type="select" label="logging, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="email" type="text"  label="email, character" value=""/>
+			<param name="assembly" type="text"  label="assembly, character" value="hg19"/>
+			<param name="columnsPairing" type="text"  label="columns.pairing, integer vector" value=""/>
+			<param name="analyzeSites" type="select" label="analyze.sites, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="regionTypes" type="text"  label="region.types, character vector" value="tiling,genes,promoters,cpgislands"/>
+			<param name="identifiersColumn" type="text"  label="identifiers.column, character" value=""/>
+			<param name="pointsCategory" type="text"  label="points.category, integer vector" value="16,17,3,15,4,7,8"/>
+			<param name="colorsCategory" type="text"  label="colors.category, character vector" value="#1B9E77,#D95F02,#7570B3,#E7298A,#66A61E,#E6AB02,#A6761D,#666666"/>
+			<param name="colorsGradient" type="text"  label="colors.gradient, character vector" value="#132B43,#56B1F7"/>
+			<param name="minGroupSize" type="text"  label="min.group.size, integer" value="2"/>
+			<param name="maxGroupCount" type="text"  label="max.group.count, integer" value="8"/>
+			<param name="gzLargeFiles" type="select" label="gz.large.files, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="strandSpecific" type="select" label="strand.specific, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="replicateIdColumn" type="text"  label="replicate.id.column, character" value=""/>
+			<param name="loadingNormalization" type="select" label="loading.normalization, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="loadingDefaultDataType" type="text"  label="loading.default.data.type, character" value="idat.dir"/>
+			<param name="loadingTableSeparator" type="text"  label="loading.table.separator, character" value=","/>
+			<param name="loadingBedStyle" type="text"  label="loading.bed.style, character" value="BisSNP"/>
+			<param name="loadingBedColumns" type="text"  label="loading.bed.columns, integer vector" value="1,2,3,6,4,5"/>
+			<param name="loadingBedFrameShift" type="text"  label="loading.bed.frame.shift, integer" value="1"/>
+			<param name="normalizationMethod" type="text"  label="normalization.method, character" value="swan"/>
+			<param name="normalizationBackgroundMethod" type="text"  label="normalization.background.method, character" value="methylumi.noob"/>
+			<param name="qc" type="select" label="qc, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="qcBoxplots" type="select" label="qc.boxplots, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="qcBarplots" type="select" label="qc.barplots, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="qcSnpHeatmap" type="select" label="qc.snp.heatmap, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="qcSnpHeatmap" type="select" label="qc.snp.heatmap, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="qcSnpBoxplot" type="select" label="qc.snp.boxplot, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="qcSnpBarplot" type="select" label="qc.snp.barplot, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="qcSampleBatchSize" type="text"  label="qc.sample.batch.size, integer" value="500"/>
+			<param name="filteringContextRemoval" type="text"  label="filtering.context.removal, character vector" value="CC,CAG,CAH,CTG,CTH,Other"/>
+			<param name="filteringSnp" type="select" label="filtering.snp, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="filteringSnpFrequency" type="text"  label="filtering.snp.frequency, double" value="0.01"/>
+			<param name="filteringSnpAccepted" type="text"  label="filtering.snp.accepted, integer" value="2"/>
+			<param name="filteringSexChromosomesRemoval" type="select" label="filtering.sex.chromosomes.removal, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="filteringMissingValueQuantile" type="text"  label="filtering.missing.value.quantile, double" value="1"/>
+			<param name="filteringCoverageThreshold" type="text"  label="filtering.coverage.threshold, integer" value="5"/>
+			<param name="filteringLowCoverageMasking" type="select" label="filtering.low.coverage.masking, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="filteringHighCoverageOutliers" type="select" label="filtering.high.coverage.outliers, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="filteringGreedycut" type="select" label="filtering.greedycut, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="filteringGreedycutPvalueThreshold" type="text"  label="filtering.greedycut.pvalue.threshold, double" value="0.05"/>
+			<param name="filteringGreedycutRcTies" type="text"  label="filtering.greedycut.rc.ties, character" value="row"/>
+			<param name="filteringDeviationThreshold" type="text"  label="filtering.deviation.threshold, double" value="0"/>
+			<param name="batch" type="select" label="batch, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="batchDreductionColumns" type="text"  label="batch.dreduction.columns, integer vector" value=""/>
+			<param name="batchPrincipalComponents" type="text"  label="batch.principal.components, integer" value="8"/>
+			<param name="batchCorrelationColumns" type="text"  label="batch.correlation.columns, integer vector" value=""/>
+			<param name="batchCorrelationPvalueThreshold" type="text"  label="batch.correlation.pvalue.threshold, double" value="0.01"/>
+			<param name="batchCorrelationPermutations" type="text"  label="batch.correlation.permutations, integer" value="10000"/>
+			<param name="batchCorrelationQc" type="select" label="batch.correlation.qc, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="profiles" type="select" label="profiles, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="profilesBetaDistribution" type="select" label="profiles.beta.distribution, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="profilesIntersample" type="select" label="profiles.intersample, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="profilesDeviationPlots" type="select" label="profiles.deviation.plots, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="profilesColumns" type="text"  label="profiles.columns, integer vector" value=""/>
+			<param name="profilesClustering" type="select" label="profiles.clustering, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="profilesClusteringTopProbes" type="text"  label="profiles.clustering.top.probes, integer vector" value="1000"/>
+			<param name="regionProfilesTypes" type="text"  label="region.profiles.types, character vector" value="genes,promoters,cpgislands"/>
+			<param name="differential" type="select" label="differential, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="differentialPermutations" type="text"  label="differential.permutations, integer" value="0"/>
+			<param name="differentialComparisonColumns" type="text"  label="differential.comparison.columns, integer vector" value=""/>
+			<param name="differentialComparisonColumnsAllPairwise" type="text"  label="differential.comparison.columns.all.pairwise, integer vector" value=""/>
+			<param name="differentialEnrichment" type="select" label="differential.enrichment, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="exportToUcsc" type="text"  label="export.to.ucsc, character vector" value="bigBed,bigWig"/>
+			<param name="exportToBed" type="select" label="export.to.bed, logical" value="1">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="exportToCsv" type="select" label="export.to.csv, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+			<param name="exportTypes" type="text"  label="export.types, character vector" value="sites"/>
+			<param name="colors3Gradient" type="text"  label="colors.3.gradient, character vector" value="#832424,#FFFFFF,#3A3A98"/>
+			<param name="loggingMemory" type="select" label="logging.memory, logical" value="0">
+				<option value="True">True</option>
+				<option value="False">False</option>
+			</param>
+ 		</when>
+    </conditional>
+  </inputs>
+  
+  <outputs>
+	<data format="html" name="html_file" label="index.html" />
+  </outputs>
+
+  <!--
+  <tests>
+    <test>
+      <param name="input" value="fa_gc_content_input.fa"/>
+      <output name="out_file1" file="fa_gc_content_output.txt"/>
+    </test>
+  </tests>  -->
+
+  <help>
+	RnBeads is an R-package for comprehensive analysis of Illumina Infinium HumanMethylation450 BeadChip data. It extends previous approaches for this type of data analysis by high throughput capabilities, as well as presenting results in a comprehensive, highly interpretable fashion.
+  </help>
+
+</tool>
\ No newline at end of file
diff -r 12b2947c0905 -r 450c8ff1d11a rnbeads_galaxy_wrapper.sh
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/rnbeads_galaxy_wrapper.sh	Fri Jul 05 09:08:41 2013 -0400
@@ -0,0 +1,27 @@
+#!/bin/bash
+random_hash=`tr -dc "[:alpha:]" < /dev/urandom | head -c 8`
+Rscript --no-save $(dirname $(readlink -f $0))/RnBeadsGalaxy.R $* > /tmp/rnbeads_${random_hash}.stdout 2>/tmp/rnbeads_${random_hash}.stderr 
+#Rscript --no-save \$R_SCRIPTS_PATH/RnBeadsGalaxy.R $*
+outfile=`echo $* | cut -d\  -f2 | sed -e "s/--output-file=//g"`
+echo $outfile
+#outdir=`echo $* | sed -e "s/.*--report-dir=\(.*\)[[:blank:]].*/\1/g"`
+
+
+errl=`cat /tmp/rnbeads_${random_hash}.stderr | grep -e "[E|e]rror" | wc -l`
+if [ "$errl" -gt 0 ]
+then
+	cat /tmp/rnbeads_${random_hash}.stdout >&2
+	#cat $outdir/analysis.log >&2
+	cat /tmp/rnbeads_${random_hash}.stderr >&2
+	exit 3
+else
+	echo "<html><body>" >> $outfile
+	echo "<a href=\"index.html\">RnBeads report</a>" >> $outfile
+	echo "<br/><br/>Output was generated during the execution:<br/>" >> $outfile
+	echo "<pre>" >> outfile
+	cat /tmp/rnbeads_${random_hash}.stdout |sed -e "s/$/<br\/>/g" >> $outfile
+	echo "</pre>" >> outfile
+	echo "</p>" >> $outfile
+	echo "</body></html>" >> $outfile
+	exit 0
+fi
\ No newline at end of file
diff -r 12b2947c0905 -r 450c8ff1d11a rnbeads_repos.txt.sample
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/rnbeads_repos.txt.sample	Fri Jul 05 09:08:41 2013 -0400
@@ -0,0 +1,1 @@
+repo1	Repository1	/data/iscan/idats
\ No newline at end of file
diff -r 12b2947c0905 -r 450c8ff1d11a tool_data_table_conf.xml.sample
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_data_table_conf.xml.sample	Fri Jul 05 09:08:41 2013 -0400
@@ -0,0 +1,6 @@
+<tables>
+	<table name="rnbeads_repos" comment_char="#">
+       		<columns>id, name, value</columns>
+        	<file path="tool-data/rnbeads_repos.txt" />
+	</table>
+</tables>
\ No newline at end of file
diff -r 12b2947c0905 -r 450c8ff1d11a tool_dependencies.xml
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/tool_dependencies.xml	Fri Jul 05 09:08:41 2013 -0400
@@ -0,0 +1,22 @@
+<?xml version="1.0"?>
+<tool_dependency>
+    <set_environment version="1.0">
+        <environment_variable name="R_SCRIPTS_PATH" action="set_to">$REPOSITORY_INSTALL_DIR</environment_variable>
+    </set_environment>
+    <package name="R" version="3.0.0">
+        <install version="1.0">
+            <actions>
+                <action type="download_by_url">http://CRAN.R-project.org/src/base/R-3/R-3.0.0.tar.gz</action>
+                <action type="shell_command">./configure --prefix=$INSTALL_DIR --with-x=no</action>
+                <action type="shell_command">make</action>
+                <action type="set_environment">
+                    <environment_variable name="PATH" action="prepend_to">$INSTALL_DIR/bin</environment_variable>
+                </action>
+                <action type="shell_command">R --no-save &lt;&lt;&lt; "install.packages("getopt"); source('http://rnbeads.mpi-inf.mpg.de')"</action>
+            </actions>
+        </install>
+        <readme>
+				You need a FORTRAN compiler or perhaps f2c in addition to a C compiler to build R.
+        </readme>
+    </package>
+</tool_dependency>
\ No newline at end of file