# HG changeset patch # User peterjc # Date 1379499207 14400 # Node ID 528ba9c896e0cf94127a4fa42d937e436ea0291d # Parent 95a632a719516eed659a9c7d459e7a670d2b3f3d Uploaded v0.0.8, MIT licence and reST for README, citation information, development moved to GitHub diff -r 95a632a71951 -r 528ba9c896e0 tools/fastq/fastq_paired_unpaired.py --- a/tools/fastq/fastq_paired_unpaired.py Tue Apr 30 14:08:37 2013 -0400 +++ b/tools/fastq/fastq_paired_unpaired.py Wed Sep 18 06:13:27 2013 -0400 @@ -9,10 +9,10 @@ Note that the FASTQ variant is unimportant (Sanger, Solexa, Illumina, or even Color Space should all work equally well). -This script is copyright 2010-2011 by Peter Cock, The James Hutton Institute +This script is copyright 2010-2013 by Peter Cock, The James Hutton Institute (formerly SCRI), Scotland, UK. All rights reserved. -See accompanying text file for licence details (MIT/BSD style). +See accompanying text file for licence details (MIT license). """ import os import sys @@ -20,7 +20,7 @@ from galaxy_utils.sequence.fastq import fastqReader, fastqWriter if "-v" in sys.argv or "--version" in sys.argv: - print "Version 0.0.6" + print "Version 0.0.8" sys.exit(0) def stop_err(msg, err=1): diff -r 95a632a71951 -r 528ba9c896e0 tools/fastq/fastq_paired_unpaired.rst --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/fastq/fastq_paired_unpaired.rst Wed Sep 18 06:13:27 2013 -0400 @@ -0,0 +1,113 @@ +Galaxy tool to divide FASTQ files into paired and unpaired reads +================================================================ + +This tool is copyright 2010-2013 by Peter Cock, The James Hutton Institute +(formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved. +See the licence text below (MIT licence). + +This tool is a short Python script which divides a FASTQ file into paired +reads, and single or orphan reads. You can have separate files for the +forward/reverse reads, or have them interleaved in a single file. + +Note that the FASTQ variant is unimportant (Sanger, Solexa, Illumina, or even +Color Space should all work equally well). + +This tool is available from the Galaxy Tool Shed at: +http://toolshed.g2.bx.psu.edu/view/peterjc/fastq_paired_unpaired + + +Automated Installation +====================== + +This should be straightforward, Galaxy should automatically download and install +the tool from the Galaxy Tool Shed, and run the unit tests + + +Manual Installation +=================== + +There are just two files to install: + +* fastq_paired_unpaired.py (the Python script) +* fastq_paired_unpaired.xml (the Galaxy tool definition) + +The suggested location is in the Galaxy folder tools/fastq next to other FASTQ +tools provided with Galaxy. + +You will also need to modify the tools_conf.xml file to tell Galaxy to offer +the tool. One suggested location is next to the fastq_filter.xml entry. Simply +add the line:: + + + +That's it. + + +History +======= + +======= ====================================================================== +Version Changes +------- ---------------------------------------------------------------------- +v0.0.1 - Initial version, using Biopython +v0.0.2 - Help text; cope with multiple pairs per template +v0.0.3 - Galaxy XML wrappers added +v0.0.4 - Use Galaxy library to handle FASTQ files (avoid Biopython dependency) +v0.0.5 - Handle Illumina 1.8 style pair names +v0.0.6 - Record script version when run from Galaxy + - Added unit test (FASTQ file using Sanger naming) +v0.0.7 - Link to Tool Shed added to help text and this documentation. +v0.0.8 - Use reStructuredText for this README file. + - Adopt standard MIT License. + - Updated citation information (Cock et al. 2013). +======= ====================================================================== + + +Developers +========== + +This script and other tools for filtering FASTA, FASTQ and SFF files were +initially developed on the following hg branch: +http://bitbucket.org/peterjc/galaxy-central/src/fasta_filter + +Development has now moved to a dedicated GitHub repository: +https://github.com/peterjc/pico_galaxy/tree/master/tools/ + +For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball use +the following command from the Galaxy root folder:: + + $ tar -czf fastq_paired_unpaired.tar.gz tools/fastq/fastq_paired_unpaired.* test-data/sanger-pairs-*.fastq + +Check this worked:: + + $ tar -tzf fastq_paired_unpaired.tar.gz + tools/fastq/fastq_paired_unpaired.py + tools/fastq/fastq_paired_unpaired.rst + tools/fastq/fastq_paired_unpaired.xml + test-data/sanger-pairs-forward.fastq + test-data/sanger-pairs-interleaved.fastq + test-data/sanger-pairs-mixed.fastq + test-data/sanger-pairs-reverse.fastq + test-data/sanger-pairs-singles.fastq + + +Licence (MIT) +============= + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in +all copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN +THE SOFTWARE. diff -r 95a632a71951 -r 528ba9c896e0 tools/fastq/fastq_paired_unpaired.txt --- a/tools/fastq/fastq_paired_unpaired.txt Tue Apr 30 14:08:37 2013 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,88 +0,0 @@ -Galaxy tool to divide FASTQ files into paired and unpaired reads -================================================================ - -This tool is copyright 2010 by Peter Cock, SCRI, UK. All rights reserved. -See the licence text below. - -This tool is a short Python script (using the Biopython library functions) which -divides a FASTQ file into paired reads, and single or orphan reads. You can have -separate files for the forward/reverse reads, or have them interleaved in a -single file. - -Note that the FASTQ variant is unimportant (Sanger, Solexa, Illumina, or even -Color Space should all work equally well). - -There are just two files to install: - -* fastq_paired_unpaired.py (the Python script) -* fastq_paired_unpaired.xml (the Galaxy tool definition) - -The suggested location is in the Galaxy folder tools/fastq next to other FASTQ -tools provided with Galaxy. - -You will also need to modify the tools_conf.xml file to tell Galaxy to offer -the tool. One suggested location is next to the fastq_filter.xml entry. Simply -add the line: - - - -That's it. - - -History -======= - -v0.0.1 - Initial version, using Biopython -v0.0.2 - Help text; cope with multiple pairs per template -v0.0.3 - Galaxy XML wrappers added -v0.0.4 - Use Galaxy library to handle FASTQ files (avoid Biopython dependency) -v0.0.5 - Handle Illumina 1.8 style pair names -v0.0.6 - Record script version when run from Galaxy - - Added unit test (FASTQ file using Sanger naming) - - -Developers -========== - -This script and other tools for filtering FASTA, FASTQ and SFF files are -currently being developed on the following hg branch: -http://bitbucket.org/peterjc/galaxy-central/src/fasta_filter - -For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball use -the following command from the Galaxy root folder: - -$ tar -czf fastq_paired_unpaired.tar.gz tools/fastq/fastq_paired_unpaired.* test-data/sanger-pairs-*.fastq - -Check this worked: - -$ tar -tzf fastq_paired_unpaired.tar.gz -fastq/fastq_paired_unpaired.py -fastq/fastq_paired_unpaired.txt -fastq/fastq_paired_unpaired.xml -test-data/sanger-pairs-forward.fastq -test-data/sanger-pairs-interleaved.fastq -test-data/sanger-pairs-mixed.fastq -test-data/sanger-pairs-reverse.fastq -test-data/sanger-pairs-singles.fastq - - -Licence (MIT/BSD style) -======================= - -Permission to use, copy, modify, and distribute this software and its -documentation with or without modifications and for any purpose and -without fee is hereby granted, provided that any copyright notices -appear in all copies and that both those copyright notices and this -permission notice appear in supporting documentation, and that the -names of the contributors or copyright holders not be used in -advertising or publicity pertaining to distribution of the software -without specific prior permission. - -THE CONTRIBUTORS AND COPYRIGHT HOLDERS OF THIS SOFTWARE DISCLAIM ALL -WARRANTIES WITH REGARD TO THIS SOFTWARE, INCLUDING ALL IMPLIED -WARRANTIES OF MERCHANTABILITY AND FITNESS, IN NO EVENT SHALL THE -CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY SPECIAL, INDIRECT -OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER RESULTING FROM LOSS -OF USE, DATA OR PROFITS, WHETHER IN AN ACTION OF CONTRACT, NEGLIGENCE -OR OTHER TORTIOUS ACTION, ARISING OUT OF OR IN CONNECTION WITH THE USE -OR PERFORMANCE OF THIS SOFTWARE. diff -r 95a632a71951 -r 528ba9c896e0 tools/fastq/fastq_paired_unpaired.xml --- a/tools/fastq/fastq_paired_unpaired.xml Tue Apr 30 14:08:37 2013 -0400 +++ b/tools/fastq/fastq_paired_unpaired.xml Wed Sep 18 06:13:27 2013 -0400 @@ -1,4 +1,4 @@ - + using the read name suffices fastq_paired_unpaired.py --version @@ -97,5 +97,17 @@ * WTSI_1055_4p17.p1kapIBF paired with WTSI_1055_4p17.q1kapIBR * WTSI_1055_4p17.p1kpIBF paired with WTSI_1055_4p17.q1kpIBR +**References** + +If you use this Galaxy tool in work leading to a scientific publication please +cite the following paper: + +Peter J.A. Cock, Björn A. Grüning, Konrad Paszkiewicz and Leighton Pritchard (2013). +Galaxy tools and workflows for sequence analysis with applications +in molecular plant pathology. PeerJ 1:e167 +http://dx.doi.org/10.7717/peerj.167 + +This tool is available to install into other Galaxy Instances via the Galaxy +Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/fastq_paired_unpaired