Mercurial > repos > peterjc > mira4_assembler
view tools/mira4/mira4_bait.py @ 0:6a88b42ce6b9 draft
Uploaded v0.0.4, previously only on the TestToolShed
author | peterjc |
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date | Fri, 21 Nov 2014 06:42:56 -0500 |
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#!/usr/bin/env python """A simple wrapper script to call MIRA4's mirabait and collect its output. """ import os import sys import subprocess import shutil import time WRAPPER_VER = "0.0.1" #Keep in sync with the XML file def stop_err(msg, err=1): sys.stderr.write(msg+"\n") sys.exit(err) def get_version(mira_binary): """Run MIRA to find its version number""" # At the commend line I would use: mira -v | head -n 1 # however there is some pipe error when doing that here. cmd = [mira_binary, "-v"] try: child = subprocess.Popen(cmd, stdout=subprocess.PIPE, stderr=subprocess.STDOUT) except Exception, err: sys.stderr.write("Error invoking command:\n%s\n\n%s\n" % (" ".join(cmd), err)) sys.exit(1) ver, tmp = child.communicate() del child #Workaround for -v not working in mirabait 4.0RC4 if "invalid option" in ver.split("\n", 1)[0]: for line in ver.split("\n", 1): if " version " in line: line = line.split() return line[line.index("version")+1].rstrip(")") stop_err("Could not determine MIRA version:\n%s" % ver) return ver.split("\n", 1)[0] try: mira_path = os.environ["MIRA4"] except KeyError: stop_err("Environment variable $MIRA4 not set") mira_binary = os.path.join(mira_path, "mirabait") if not os.path.isfile(mira_binary): stop_err("Missing mirabait under $MIRA4, %r\nFolder contained: %s" % (mira_binary, ", ".join(os.listdir(mira_path)))) mira_ver = get_version(mira_binary) if not mira_ver.strip().startswith("4.0"): stop_err("This wrapper is for MIRA V4.0, not:\n%s" % mira_ver) if "-v" in sys.argv or "--version" in sys.argv: print "%s, MIRA wrapper version %s" % (mira_ver, WRAPPER_VER) sys.exit(0) format, output_choice, strand_choice, kmer_length, min_occurance, bait_file, in_file, out_file = sys.argv[1:] if format.startswith("fastq"): format = "fastq" elif format == "mira": format = "maf" elif format != "fasta": stop_err("Was not expected format %r" % format) assert out_file.endswith(".dat") out_file_stem = out_file[:-4] cmd_list = [mira_binary, "-f", format, "-t", format, "-k", kmer_length, "-n", min_occurance, bait_file, in_file, out_file_stem] if output_choice == "pos": pass elif output_choice == "neg": #Invert the selection... cmd_list.insert(1, "-i") else: stop_err("Output choice should be 'pos' or 'neg', not %r" % output_choice) if strand_choice == "both": pass elif strand_choice == "fwd": #Ingore reverse strand... cmd_list.insert(1, "-r") else: stop_err("Strand choice should be 'both' or 'fwd', not %r" % strand_choice) cmd = " ".join(cmd_list) #print cmd start_time = time.time() try: #Run MIRA child = subprocess.Popen(cmd_list, stdout=subprocess.PIPE, stderr=subprocess.STDOUT) except Exception, err: log_manifest(manifest) sys.stderr.write("Error invoking command:\n%s\n\n%s\n" % (cmd, err)) sys.exit(1) #Use .communicate as can get deadlocks with .wait(), stdout, stderr = child.communicate() assert stderr is None # Due to way we ran with subprocess run_time = time.time() - start_time return_code = child.returncode print "mirabait took %0.2f minutes" % (run_time / 60.0) if return_code: sys.stderr.write(stdout) stop_err("Return error code %i from command:\n%s" % (return_code, cmd), return_code) #Capture output out_tmp = out_file_stem + "." + format if not os.path.isfile(out_tmp): sys.stderr.write(stdout) stop_err("Missing output file from mirabait: %s" % out_tmp) shutil.move(out_tmp, out_file)