Mercurial > repos > peterjc > mira_assembler
view tools/sr_assembly/mira.py @ 7:4d3f94dfb857 draft
Uploaded v0.0.6, tell MIRA to use /tmp for temporary files, and ignore long read names (to prevent it aborting).
author | peterjc |
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date | Thu, 14 Feb 2013 06:26:32 -0500 |
parents | 3e7eca1f5d04 |
children |
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#!/usr/bin/env python """A simple wrapper script to call MIRA and collect its output. """ import os import sys import subprocess import shutil import time WRAPPER_VER = "0.0.5" #Keep in sync with the XML file def stop_err(msg, err=1): sys.stderr.write(msg+"\n") sys.exit(err) def get_version(): """Run MIRA to find its version number""" # At the commend line I would use: mira -v | head -n 1 # however there is some pipe error when doing that here. try: child = subprocess.Popen(["mira", "-v"], stdout=subprocess.PIPE, stderr=subprocess.STDOUT) except Exception, err: sys.stderr.write("Error invoking command:\n%s\n\n%s\n" % (" ".join(cmd), err)) sys.exit(1) ver, tmp = child.communicate() del child return ver.split("\n", 1)[0] mira_ver = get_version() if "V3.4." not in mira_ver: stop_err("This wrapper is for MIRA V3.4, not %s" % mira_ver) if "-v" in sys.argv: print "MIRA wrapper version %s," % WRAPPER_VER print mira_ver sys.exit(0) def collect_output(temp, name): n3 = (temp, name, name, name) f = "%s/%s_assembly/%s_d_results" % (temp, name, name) if not os.path.isdir(f): stop_err("Missing output folder") if not os.listdir(f): stop_err("Empty output folder") missing = [] for old, new in [("%s/%s_out.unpadded.fasta" % (f, name), out_fasta), ("%s/%s_out.unpadded.fasta.qual" % (f, name), out_qual), ("%s/%s_out.wig" % (f, name), out_wig), ("%s/%s_out.caf" % (f, name), out_caf), ("%s/%s_out.ace" % (f, name), out_ace)]: if not os.path.isfile(old): missing.append(os.path.splitext(old)[-1]) else: shutil.move(old, new) if missing: stop_err("Missing output files: %s" % ", ".join(missing)) def clean_up(temp, name): folder = "%s/%s_assembly" % (temp, name) if os.path.isdir(folder): shutil.rmtree(folder) #TODO - Run MIRA in /tmp or a configurable directory? #Currently Galaxy puts us somewhere safe like: #/opt/galaxy-dist/database/job_working_directory/846/ temp = "." name, out_fasta, out_qual, out_ace, out_caf, out_wig, out_log = sys.argv[1:8] start_time = time.time() cmd_list =sys.argv[8:] cmd = " ".join(cmd_list) assert os.path.isdir(temp) d = "%s_assembly" % name assert not os.path.isdir(d), "Path %s already exists" % d try: #Check path access os.mkdir(d) except Exception, err: sys.stderr.write("Error making directory %s\n%s" % (d, err)) sys.exit(1) #print os.path.abspath(".") #print cmd handle = open(out_log, "w") try: #Run MIRA child = subprocess.Popen(cmd_list, stdout=handle, stderr=subprocess.STDOUT) except Exception, err: sys.stderr.write("Error invoking command:\n%s\n\n%s\n" % (cmd, err)) #TODO - call clean up? handle.write("Error invoking command:\n%s\n\n%s\n" % (cmd, err)) handle.close() sys.exit(1) #Use .communicate as can get deadlocks with .wait(), stdout, stderr = child.communicate() assert not stdout and not stderr #Should be empty as sent to handle run_time = time.time() - start_time return_code = child.returncode handle.write("\n\nMIRA took %0.2f minutes\n" % (run_time / 60.0)) print "MIRA took %0.2f minutes" % (run_time / 60.0) if return_code: handle.write("Return error code %i from command:\n" % return_code) handle.write(cmd + "\n") handle.close() clean_up(temp, name) stop_err("Return error code %i from command:\n%s" % (return_code, cmd), return_code) handle.close() #print "Collecting output..." collect_output(temp, name) #print "Cleaning up..." clean_up(temp, name) print "Done"