Mercurial > repos > peterjc > secreted_protein_workflow
diff README.rst @ 2:3a0c0d1c388f draft
Uploaded v0.0.2, updated tool versions to solve warning from core Galaxy filter tool being updated. No functional changes.
author | peterjc |
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date | Wed, 21 Aug 2013 11:58:54 -0400 |
parents | 606da4e1d925 |
children | 72f03c2102ee |
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--- a/README.rst Mon Aug 19 11:37:04 2013 -0400 +++ b/README.rst Wed Aug 21 11:58:54 2013 -0400 @@ -4,12 +4,26 @@ It runs SignalP v3.0 (Bendtsen et al. 2004) and selects only proteins with a strong predicted signal peptide, and then runs TMHMM v2.0 (Krogh et al. 2001) on those, and selects only proteins without a predicted trans-membrane helix. -This workflow was used in Kikuchi et al (2001), and is a simplification of -the candidate effector protocol described in Jones et al (2009). +This workflow was used in Kikuchi et al. (2011), and is a simplification of +the candidate effector protocol described in Jones et al. (2009). See http://www.galaxyproject.org for information about the Galaxy Project. +Sample Data +=========== + +This workflow was developed and run on several nematode species. For example, +try the protein set for Bursaphelenchus xylophilus (Kikuchi et al. 2011): + +ftp://ftp.sanger.ac.uk/pub/pathogens/Bursaphelenchus/xylophilus/Assembly-v1.2/BUX.v1.2.genedb.protein.fa.gz + +You can upload this directly into Galaxy via this URL. Galaxy will handle +removing the gzip compression to give you the FASTA protein file which has +18,074 sequences. The expected result (selecting organism type Eukaryote) +is a FASTA protein file of 2,297 predicted secreted protein sequences. + + Citation ======== @@ -78,6 +92,19 @@ installation (SignalP v3.0 and TMHMM v2.0). +History +======= + +======= ====================================================================== +Version Changes +------- ---------------------------------------------------------------------- +v0.0.1 - Initial release to Tool Shed (May, 2013) + - Expanded README file to include example data +v0.0.2 - Updated versions of the tools used, inclulding core Galaxy Filter + tool to avoid warning about new ``header_lines`` parameter. +======= ====================================================================== + + Developers ==========