Mercurial > repos > petr-novak > dante_ltr
annotate dante_ltr_search.xml @ 1:c1498f679b50 draft
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author | petr-novak |
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date | Wed, 09 Mar 2022 09:31:31 +0000 |
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children | f131886ea194 |
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1 <tool id="dante_ltr_search" name="DANTE_LTR transposable element identification" version="0.1.0" python_template_version="3.5"> |
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2 <requirements> |
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3 <requirement type="package">blast</requirement> |
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4 <requirement type="package">r-optparse</requirement> |
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5 <requirement type="package">bioconductor-bsgenome</requirement> |
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6 <requirement type="package">bioconductor-biostrings</requirement> |
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7 <requirement type="package">bioconductor-rtracklayer</requirement> |
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8 |
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9 </requirements> |
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10 <command detect_errors="exit_code"><![CDATA[ |
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11 Rscript ${__tool_directory__}/extract_putative_ltr.R --gff3 '$dante' --reference_sequence '$reference' --output output --cpu 32 |
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12 && |
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13 mv output.gff3 $te_ltr_gff |
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14 ]]></command> |
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15 <inputs> |
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16 <param type="data" name="dante" format="gff3" label="Filtered gff3 output from DANTE pipeline"/> |
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17 <param type="data" name="reference" format="fasta" label="Reference sequence matching DANTE output" /> |
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18 </inputs> |
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19 <outputs> |
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20 <data name="te_ltr_gff" format="gff3" label="Annotation of detected LTR transposable elements |
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21 based on the annotation $dante.hid and reference $reference.hid" /> |
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22 </outputs> |
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23 <help><![CDATA[ |
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24 This tool uses output from DANTE annotation pipeline to identify full length LTR |
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25 transposable elements. Output is in the GFF3 format and include annotation of |
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26 5' and 3' Longe Terminal Repeats, Target Site Duplication (TSD) and primer binding site (PBS). |
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27 |
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28 All identified elements contains complete set of protein domains as defined in |
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29 REXdb_. Based on the results detection structural feature, |
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30 elements falls into four categories: |
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31 |
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32 - elements with domains, 5'LTR, 3'LTR, TSD and PBS |
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33 - elements with domains, 5'LTR, 3'LTR and PBS (TSD was not found) |
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34 - elements with domains, 5' LTR, 3'LTR, TSD (PBS was not found) |
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35 - elements with protein domains, 5'LTR and 3'LTR (PBS and LDS were not found) |
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36 |
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37 .. _REXdb: https://doi.org/10.1186/s13100-018-0144-1 |
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38 |
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39 ]]></help> |
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40 </tool> |