Mercurial > repos > petr-novak > dante_ltr
diff README.md @ 0:7b0bbe7477c4 draft
"planemo upload commit 92c684dff3b377c8c08654c7f3d46a133385e3e0-dirty"
author | petr-novak |
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date | Tue, 08 Mar 2022 13:24:33 +0000 |
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children | 9de392f2fc02 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.md Tue Mar 08 13:24:33 2022 +0000 @@ -0,0 +1,51 @@ +# dante_ltr + +Tool for identification of complete LTR retrotransposons based on analysis of protein +domains identified by DANTE tool. + +## Installation: + +```shell +conda create -n dante_ltr -c bioconda -c conda-forge -c petrnovak dante_ltr +``` +## Usage + +```shell +Usage: ./extract_putative_ltr.R COMMAND [OPTIONS] + + +Options: + -g GFF3, --gff3=GFF3 + gff3 with dante results + + -s REFERENCE_SEQUENCE, --reference_sequence=REFERENCE_SEQUENCE + reference sequence as fasta + + -o OUTPUT, --output=OUTPUT + output file path and prefix + + -c NUMBER, --cpu=NUMBER + Number of cpu to use [default 5] + + -h, --help + Show this help message and exit +``` + +## Example +```shell +mkdir -p tmp +./extract_putative_ltr.R -g test_data/sample_DANTE.gff3 -s test_data/sample_genome.fasta -o tmp/ltr_annotation +``` + +## Output files + + +### Output of script `extract_putative_ltr.R`: + + +- `prefix.gff3` - annotation of all identified elements +- `prefix_DL.fasta` - elements with protein **d**omains and **L**TR +- `prefix_DLTP.fasta` - elements with **d**omains, **L**TR, **T**SD and **P**BS +- `prefix_DLP.fasta` - elements with **d**omains, **L**TR and **P**BS +- `prefix_DLT.fasta` - elements with **d**omains, **L**TR, **T**SD +- `prefix_statistics.csv` - number of elements in individual categories