Mercurial > repos > petr-novak > repeat_annotation_pipeline2
view clean_rm_output.R @ 2:3f8ae272f4f3 draft
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author | petr-novak |
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date | Thu, 07 Oct 2021 07:29:59 +0000 |
parents | cf3cea0a3039 |
children | e955b40ad3a4 |
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#!/usr/bin/env Rscript suppressPackageStartupMessages(library(rtracklayer)) gff_cleanup = function(gff){ ## remove overlapin annotation track - assign new annot gff_disjoin = disjoin(gff, with.revmap=TRUE) ## append annotation: gff_names = mclapply(as.list(gff_disjoin$revmap), FUN = function(x)gff$Name[x], mc.cores = 8) gff_strands = mclapply(as.list(gff_disjoin$revmap), FUN = function(x)strand(gff[x]), mc.cores = 8) new_annot = sapply(sapply(gff_names, unique), paste, collapse="|") strand_attribute = sapply(sapply(gff_strands, unique), paste, collapse="|") gff_disjoin$strands=strand_attribute gff_disjoin$source="RM" gff_disjoin$type="repeat" gff_disjoin$score=NA gff_disjoin$phase=NA gff_disjoin$Name=new_annot gff_disjoin$revmap=NULL return(gff_disjoin) } infile = commandArgs(T)[1] outfile = commandArgs(T)[2] ## infile = "./test_data/raw_rm.out" rm_out = read.table(infile, as.is=TRUE, sep="", skip = 2, fill=TRUE, header=FALSE) gff = GRanges(seqnames = rm_out$V5, ranges = IRanges(start = rm_out$V6, end=rm_out$V7)) # repeat class after # symbol - syntax 1 gff$Name=rm_out$V11 ## is repeat type is specifies by double underscore: ## then rm_out$V11 is unspecified if (any(rm_out$V11 == "Unspecified")){ ## set Name from prefix ## TODO inc = rm_out$V11 == "Unspecified" Name = gsub("__.+","",rm_out$V10) # chanche Usnpsecified to new name gff$Name[inc] = Name } ## join neighbors with the same annotation, disregard strand! result <- unlist(reduce(split(gff, gff$Name))) result$Name <- names(result) ## TODO ## identify conflicting annotation, replace by LCA but keep origin list of classifications gff_out = sortSeqlevels(result) gff_out = sort(gff_out) gff_out$type = "repeat_region" gff_out$source = "RepeatMasker_parsed" gff_out$ID=paste0(gff_out$Name, "_", seq_along(gff_out$Name)) export(gff_out, format = "gff3", con=outfile)