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1 #!/usr/bin/env Rscript
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2 suppressPackageStartupMessages(library(rtracklayer))
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3 g = import(commandArgs(T)[1], format = "GFF")
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4 attribute_name = commandArgs(T)[2]
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5
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6 m = mcols(g)
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7 AN = commandArgs(T)[2]
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8 ## check if attribute is in gff:
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9 if (!AN %in% colnames(m)){
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10 stop(paste0("attribute ", AN," not in input gff\n", "Available attributes are:\n", paste(colnames(m), collapse = "\n ")))
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11 }
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12
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13 w = width(g)
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14 total_lengths = by(w, INDICES=m[,attribute_name] , sum)
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15 total_counts = by(w, INDICES=m[,attribute_name] , length)
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16 total_length_summary = do.call(rbind,as.list(by(w, INDICES=m[,attribute_name] , summary)))
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17 colnames(total_length_summary) = paste0("length.", colnames(total_length_summary))
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18
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19 d = data.frame(attribute = names(total_counts), cbind(count = total_counts, total_length=total_lengths, length=total_length_summary))
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20 colnames(d)[1] = attribute_name
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21 d = d[order(d$total_length, decreasing = TRUE),]
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22 write.table(d, sep = "\t", row.names = FALSE, quote = FALSE, file = commandArgs(T)[3])
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