Mercurial > repos > pjbriggs > amplicon_analysis_pipeline
annotate amplicon_analysis_pipeline.xml @ 4:86a12d75ebe4 draft default tip
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
author | pjbriggs |
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date | Fri, 20 Dec 2019 06:59:49 -0500 |
parents | 3ab198df8f3f |
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rev | line source |
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4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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1 <tool id="amplicon_analysis_pipeline" name="Amplicon Analysis Pipeline" version="1.3.6.0"> |
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47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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2 <description>analyse 16S rRNA data from Illumina Miseq paired-end reads</description> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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3 <requirements> |
4
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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4 <requirement type="package" version="1.3.6">amplicon_analysis_pipeline</requirement> |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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5 </requirements> |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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6 <stdio> |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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7 <exit_code range="1:" /> |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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8 </stdio> |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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9 <command><![CDATA[ |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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10 |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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11 ## Convenience variable for pipeline name |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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12 #set $pipeline_name = $pipeline.pipeline_name |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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13 |
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47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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14 ## Set the reference database name |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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15 #if str( $pipeline_name ) == "DADA2" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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16 #set reference_database_name = "silva" |
1 | 17 #else |
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86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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18 #set reference_database = $pipeline.reference_database |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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19 #if $reference_database == "-S" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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20 #set reference_database_name = "silva" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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21 #else if $reference_database == "-H" |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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22 #set reference_database_name = "homd" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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23 #else |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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24 #set reference_database_name = "gg" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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25 #end if |
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47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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26 #end if |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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27 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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28 ## Run the amplicon analysis pipeline wrapper |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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29 python $__tool_directory__/amplicon_analysis_pipeline.py |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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30 ## Set options |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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31 #if str( $forward_pcr_primer ) != "" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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32 -g "$forward_pcr_primer" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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33 #end if |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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34 #if str( $reverse_pcr_primer ) != "" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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35 -G "$reverse_pcr_primer" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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36 #end if |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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37 #if str( $trimming_threshold ) != "" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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38 -q $trimming_threshold |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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39 #end if |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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40 #if str( $sliding_window_length ) != "" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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41 -l $sliding_window_length |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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42 #end if |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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43 #if str( $minimum_overlap ) != "" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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44 -O $minimum_overlap |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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45 #end if |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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46 #if str( $minimum_length ) != "" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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47 -L $minimum_length |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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48 #end if |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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49 -P $pipeline_name |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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50 -r \${AMPLICON_ANALYSIS_REF_DATA_PATH-ReferenceData} |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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51 #if str( $pipeline_name ) != "DADA2" |
1 | 52 ${reference_database} |
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47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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53 #end if |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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54 #if str($categories_file_in) != 'None' |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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55 -c "${categories_file_in}" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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56 #end if |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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57 ## Input files |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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58 "${metatable_file_in}" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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59 ## FASTQ pairs |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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60 #if str($input_type.pairs_or_collection) == "collection" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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61 #set fastq_pairs = $input_type.fastq_collection |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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62 #else |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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63 #set fastq_pairs = $input_type.fastq_pairs |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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64 #end if |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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65 #for $fq_pair in $fastq_pairs |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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66 "${fq_pair.name}" "${fq_pair.forward}" "${fq_pair.reverse}" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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67 #end for |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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68 && |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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69 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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70 ## Collect outputs |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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71 cp Metatable_log/Metatable_mod.txt "${metatable_mod}" && |
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86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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72 #if str( $pipeline_name ) == "Vsearch" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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73 ## Vsearch-specific |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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74 cp ${pipeline_name}_OTU_tables/multiplexed_linearized_dereplicated_mc2_repset_nonchimeras_tax_OTU_table.biom "${tax_otu_table_biom_file}" && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
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75 cp Multiplexed_files/${pipeline_name}_pipeline/multiplexed_linearized_dereplicated_mc2_repset_nonchimeras_OTUs.fasta "${dereplicated_nonchimera_otus_fasta}" && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
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76 cp QUALITY_CONTROL/Reads_count.txt "$read_counts_out" && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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77 #else |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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78 ## DADA2-specific |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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79 cp ${pipeline_name}_OTU_tables/DADA2_tax_OTU_table.biom "${tax_otu_table_biom_file}" && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
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80 cp ${pipeline_name}_OTU_tables/seqs.fa "${dereplicated_nonchimera_otus_fasta}" && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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81 #end if |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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82 cp ${pipeline_name}_OTU_tables/otus.tre "${otus_tre_file}" && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
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83 cp RESULTS/${pipeline_name}_${reference_database_name}/OTUs_count.txt "${otus_count_file}" && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
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84 cp RESULTS/${pipeline_name}_${reference_database_name}/table_summary.txt "${table_summary_file}" && |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
85 cp fastqc_quality_boxplots.html "${fastqc_quality_boxplots_html}" && |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
86 |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
87 ## OTU table heatmap |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
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diff
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88 cp RESULTS/${pipeline_name}_${reference_database_name}/Heatmap.pdf "${heatmap_otu_table_pdf}" && |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
89 |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
90 ## HTML outputs |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
91 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
92 ## Phylum genus barcharts |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
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93 mkdir $phylum_genus_dist_barcharts_html.files_path && |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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94 cp -r RESULTS/${pipeline_name}_${reference_database_name}/phylum_genus_charts/charts $phylum_genus_dist_barcharts_html.files_path && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
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diff
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95 cp -r RESULTS/${pipeline_name}_${reference_database_name}/phylum_genus_charts/raw_data $phylum_genus_dist_barcharts_html.files_path && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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96 cp RESULTS/${pipeline_name}_${reference_database_name}/phylum_genus_charts/bar_charts.html "${phylum_genus_dist_barcharts_html}" && |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
diff
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97 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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98 ## Beta diversity weighted 2d plots |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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99 mkdir $beta_div_even_weighted_2d_plots.files_path && |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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100 cp -r RESULTS/${pipeline_name}_${reference_database_name}/beta_div_even/weighted_2d_plot/* $beta_div_even_weighted_2d_plots.files_path && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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101 cp RESULTS/${pipeline_name}_${reference_database_name}/beta_div_even/weighted_2d_plot/weighted_unifrac_pc_2D_PCoA_plots.html "${beta_div_even_weighted_2d_plots}" && |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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102 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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103 ## Beta diversity unweighted 2d plots |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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104 mkdir $beta_div_even_unweighted_2d_plots.files_path && |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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105 cp -r RESULTS/${pipeline_name}_${reference_database_name}/beta_div_even/unweighted_2d_plot/* $beta_div_even_unweighted_2d_plots.files_path && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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parents:
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106 cp RESULTS/${pipeline_name}_${reference_database_name}/beta_div_even/unweighted_2d_plot/unweighted_unifrac_pc_2D_PCoA_plots.html "${beta_div_even_unweighted_2d_plots}" && |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
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107 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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108 ## Alpha diversity rarefaction plots |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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109 mkdir $alpha_div_rarefaction_plots.files_path && |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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parents:
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110 cp RESULTS/${pipeline_name}_${reference_database_name}/Alpha_diversity/rarefaction_curves/rarefaction_plots.html $alpha_div_rarefaction_plots && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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111 cp -r RESULTS/${pipeline_name}_${reference_database_name}/Alpha_diversity/rarefaction_curves/average_plots $alpha_div_rarefaction_plots.files_path && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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parents:
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112 |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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parents:
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113 ## DADA2 error rate plots |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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114 #if str($pipeline_name) == "DADA2" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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parents:
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115 mkdir $dada2_error_rate_plots.files_path && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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parents:
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116 cp DADA2_OTU_tables/Error_rate_plots/error_rate_plots.html $dada2_error_rate_plots && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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117 cp -r DADA2_OTU_tables/Error_rate_plots/*.pdf $dada2_error_rate_plots.files_path && |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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118 #end if |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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119 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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120 ## Categories data |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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121 #if str($categories_file_in) != 'None' |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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122 ## Alpha diversity boxplots |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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123 mkdir $alpha_div_boxplots.files_path && |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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124 cp alpha_diversity_boxplots.html "$alpha_div_boxplots" && |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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125 cp RESULTS/${pipeline_name}_${reference_database_name}/Alpha_diversity/Alpha_diversity_boxplot/Categories_shannon/*.pdf $alpha_div_boxplots.files_path && |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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126 #end if |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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127 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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128 ## Pipeline outputs (log files etc) |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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129 mkdir $log_files.files_path && |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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130 cp Amplicon_analysis_pipeline.log $log_files.files_path && |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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131 cp pipeline.log $log_files.files_path && |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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132 cp Pipeline_outputs.txt $log_files.files_path && |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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133 cp Metatable_log/Metatable.html $log_files.files_path && |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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134 cp pipeline_outputs.html "$log_files" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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|
135 ]]></command> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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|
136 <inputs> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
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137 <param name="title" type="text" value="test" size="25" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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138 label="Title" help="Optional text that will be added to the output dataset names" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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139 <param type="data" name="metatable_file_in" format="tabular" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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|
140 label="Input Metatable.txt file" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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141 <param type="data" name="categories_file_in" format="txt" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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142 label="Input Categories.txt file" optional="true" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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143 help="(optional)" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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144 <conditional name="input_type"> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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145 <param name="pairs_or_collection" type="select" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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146 label="Input FASTQ type"> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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147 <option value="pairs_of_files">Pairs of datasets</option> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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148 <option value="collection" selected="true">Dataset pairs in a collection</option> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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149 </param> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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150 <when value="collection"> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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151 <param name="fastq_collection" type="data_collection" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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152 format="fastqsanger,fastq" collection_type="list:paired" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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153 label="Collection of FASTQ forward and reverse (R1/R2) pairs" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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154 help="Each FASTQ pair will be treated as one sample; the name of each sample will be taken from the first column of the Metatable file " /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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155 </when> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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156 <when value="pairs_of_files"> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
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157 <repeat name="fastq_pairs" title="Input fastq pairs" min="1"> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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158 <param type="text" name="name" value="" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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159 label="Final name for FASTQ pair" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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changeset
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160 <param type="data" name="fastq_r1" format="fastqsanger,fastq" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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changeset
|
161 label="FASTQ with forward reads (R1)" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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162 <param type="data" name="fastq_r2" format="fastqsanger,fastq" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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163 label="FASTQ with reverse reads (R2)" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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changeset
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164 </repeat> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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165 </when> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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parents:
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166 </conditional> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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changeset
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167 <param type="text" name="forward_pcr_primer" value="" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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changeset
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168 label="Forward PCR primer sequence" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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changeset
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169 help="Optional; must not include barcode or adapter sequence (-g)" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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changeset
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170 <param type="text" name="reverse_pcr_primer" value="" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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changeset
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171 label="Reverse PCR primer sequence" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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changeset
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172 help="Optional; must not include barcode or adapter sequence (-G)" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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173 <param type="integer" name="trimming_threshold" value="20" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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174 label="Threshold quality below which read will be trimmed" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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changeset
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175 help="Phred score; default is 20 (-q)" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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176 <param type="integer" name="minimum_overlap" value="10" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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177 label="Minimum overlap in bp between forward and reverse reads" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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178 help="Default is 10 (-O)" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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179 <param type="integer" name="minimum_length" value="200" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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180 label="Minimum length in bp to keep sequence after overlapping" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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181 help="Default is 200 (-L)" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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182 <param type="integer" name="sliding_window_length" value="10" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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changeset
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183 label="Minimum length in bp to retain a read after trimming" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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184 help="Supplied to Sickle; default is 10 (-l)" /> |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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185 <conditional name="pipeline"> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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186 <param type="select" name="pipeline_name" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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187 label="Pipeline to use for analysis"> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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188 <option value="Vsearch" selected="true" >Vsearch</option> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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189 <option value="DADA2">DADA2</option> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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190 </param> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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191 <when value="Vsearch"> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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192 <param type="select" name="reference_database" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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193 label="Reference database"> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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194 <option value="" selected="true">GreenGenes</option> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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195 <option value="-S">Silva</option> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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196 <option value="-H">Human Oral Microbiome Database (HOMD)</option> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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197 </param> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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198 </when> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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199 <when value="DADA2"> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
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200 </when> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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201 </conditional> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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202 </inputs> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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203 <outputs> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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204 <data format="tabular" name="metatable_mod" |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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205 label="${tool.name}:${title} Metatable_mod.txt" /> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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206 <data format="tabular" name="read_counts_out" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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207 label="${tool.name} (${pipeline.pipeline_name}):${title} read counts"> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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208 <filter>pipeline['pipeline_name'] == 'Vsearch'</filter> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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209 </data> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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210 <data format="biom" name="tax_otu_table_biom_file" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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211 label="${tool.name} (${pipeline.pipeline_name}):${title} tax OTU table (biom format)" /> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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212 <data format="tabular" name="otus_tre_file" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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213 label="${tool.name} (${pipeline.pipeline_name}):${title} otus.tre" /> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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214 <data format="html" name="phylum_genus_dist_barcharts_html" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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215 label="${tool.name} (${pipeline.pipeline_name}):${title} phylum genus dist barcharts HTML" /> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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216 <data format="tabular" name="otus_count_file" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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217 label="${tool.name} (${pipeline.pipeline_name}):${title} OTUs count file" /> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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218 <data format="tabular" name="table_summary_file" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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219 label="${tool.name} (${pipeline.pipeline_name}):${title} table summary file" /> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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220 <data format="fasta" name="dereplicated_nonchimera_otus_fasta" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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221 label="${tool.name} (${pipeline.pipeline_name}):${title} multiplexed linearized dereplicated mc2 repset nonchimeras OTUs FASTA" /> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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222 <data format="html" name="fastqc_quality_boxplots_html" |
4
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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223 label="${tool.name} (${pipeline.pipeline_name}):${title} FastQC per-base quality boxplots HTML" /> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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224 <data format="pdf" name="heatmap_otu_table_pdf" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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225 label="${tool.name} (${pipeline.pipeline_name}):${title} heatmap OTU table PDF" /> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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226 <data format="html" name="beta_div_even_weighted_2d_plots" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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227 label="${tool.name} (${pipeline.pipeline_name}):${title} beta diversity weighted 2D plots HTML" /> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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228 <data format="html" name="beta_div_even_unweighted_2d_plots" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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229 label="${tool.name} (${pipeline.pipeline_name}):${title} beta diversity unweighted 2D plots HTML" /> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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230 <data format="html" name="alpha_div_rarefaction_plots" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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231 label="${tool.name} (${pipeline.pipeline_name}):${title} alpha diversity rarefaction plots HTML" /> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
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232 <data format="html" name="dada2_error_rate_plots" |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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233 label="${tool.name} (${pipeline.pipeline_name}):${title} DADA2 error rate plots"> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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234 <filter>pipeline['pipeline_name'] == 'DADA2'</filter> |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
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235 </data> |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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236 <data format="html" name="alpha_div_boxplots" |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
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237 label="${tool.name} (${pipeline.pipeline_name}):${title} alpha diversity boxplots"> |
0
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238 <filter>categories_file_in is not None</filter> |
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239 </data> |
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240 <data format="html" name="log_files" |
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241 label="${tool.name} (${pipeline.pipeline_name}):${title} log files" /> |
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242 </outputs> |
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243 <tests> |
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244 </tests> |
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245 <help><![CDATA[ |
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246 |
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247 What it does |
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248 ------------ |
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249 |
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250 This pipeline has been designed for the analysis of 16S rRNA data from |
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planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
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251 Illumina Miseq (Casava >= 1.8) paired-end reads. |
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252 |
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253 Usage |
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254 ----- |
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255 |
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256 1. Preparation of the mapping file and format of unique sample id |
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257 ***************************************************************** |
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258 |
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259 Before using the amplicon analysis pipeline it would be necessary to |
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260 follow the steps as below to avoid analysis failures and ensure samples |
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261 are labelled appropriately. Sample names for the labelling are derived |
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262 from the fastq files names that are generated from the sequencing. The |
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263 labels will include everything between the beginning of the name and |
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264 the sample number (from C11 to S19 in Fig. 1) |
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265 |
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266 .. image:: Pipeline_description_Fig1.png |
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267 :height: 46 |
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268 :width: 382 |
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269 |
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270 **Figure 1** |
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271 |
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272 If analysing 16S data from multiple runs: |
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273 |
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274 The samples from different runs may have identical IDs. For example, |
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275 when sequencing the same samples twice, by chance, these could be at |
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276 the same position in both the runs. This would cause the fastq files |
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277 to have exactly the same IDs (Fig. 2). |
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278 |
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279 .. image:: Pipeline_description_Fig2.png |
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280 :height: 100 |
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281 :width: 463 |
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282 |
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283 **Figure 2** |
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284 |
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285 In case of identical sample IDs the pipeline will fail to run and |
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286 generate an error at the beginning of the analysis. |
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287 |
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288 To avoid having to change the file names, before uploading the files, |
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289 ensure that the samples IDs are not repeated. |
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290 |
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291 2. To upload the file |
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292 ********************* |
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293 |
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294 Click on **Get Data/Upload File** from the Galaxy tool panel on the |
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295 left hand side. |
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296 |
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297 From the pop-up window, choose how to upload the file. The |
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298 **Choose local file** option can be used for files up to 4Gb. Fastq files |
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299 from Illumina MiSeq will rarely be bigger than 4Gb and this option is |
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300 recommended. |
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301 |
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302 After choosing the files click **Start** to begin the upload. The window can |
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303 now be closed and the files will be uploaded onto the Galaxy server. You |
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304 will see the progress on the ``HISTORY`` panel on the right |
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305 side of the screen. The colour will change from grey (queuing), to yellow |
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306 (uploading) and finally green (uploaded). |
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307 |
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308 Once all the files are uploaded, click on the operations on multiple |
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309 datasets icon and select the fastq files that need to be analysed. |
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310 Click on the tab **For all selected...** and on the option |
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311 **Build List of Dataset pairs** (Fig. 3). |
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312 |
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313 .. image:: Pipeline_description_Fig3.png |
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314 :height: 247 |
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315 :width: 586 |
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316 |
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317 **Figure 3** |
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318 |
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319 Change the filter parameter ``_1`` and ``_2`` to be ``_R1`` and ``_R2``. |
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320 The fastq files forward R1 and reverse R2 should now appear in the |
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321 corresponding columns. |
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322 |
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323 Select **Autopair**. This creates a collection of paired fastq files for |
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324 the forward and reverse reads for each sample. The name of the pairs will |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
325 be the ones used by the pipeline. You are free to change the names at this |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
326 point as long as they are the same used in the Metatable file |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
327 (see section 3). |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
328 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
329 Name the collection and click on **create list**. This reduces the time |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
330 required to input the forward and reverse reads for each individual sample. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
331 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
332 3. Create the Metatable files |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
333 ***************************** |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
334 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
335 Metatable.txt |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
336 ~~~~~~~~~~~~~ |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
337 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
338 Click on the list of pairs you just created to see the name of the single |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
339 pairs. The name of the pairs will be the ones used by the pipeline, |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
340 therefore, these are the names that need to be used in the Metatable file. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
341 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
342 The Metatable file has to be in QIIME format. You can find a description |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
343 of it on QIIME website http://qiime.org/documentation/file_formats.html |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
344 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
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345 EXAMPLE:: |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
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346 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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changeset
|
347 #SampleID BarcodeSequence LinkerPrimerSequence Disease Gender Description |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
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348 Mock-RUN1 TAAGGCGAGCGTAAGA PsA Male Control |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
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349 Mock-RUN2 CGTACTAGGCGTAAGA PsA Male Control |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
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changeset
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350 Mock-RUN3 AGGCAGAAGCGTAAGA PsC Female Control |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
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351 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
352 Briefly: the column ``LinkerPrimerSequence`` is empty but it cannot be |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
353 deleted. The header is very important. ``#SampleID``, ``Barcode``, |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
354 ``LinkerPrimerSequence`` and ``Description`` are mandatory. Between |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
355 ``LinkerPrimerSequence`` and ``Description`` you can add as many columns |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
356 as you want. For every column a PCoA plot will be created (see |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
357 **Results** section). You can create this file in Excel and it will have |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
358 to be saved as ``Text(Tab delimited)``. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
359 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
360 During the analysis the Metatable.txt will be checked to ensure that the |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
361 file has the correct format. If necessary, this will be modified and will |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
362 be available as Metatable_corrected.txt in the history panel. If you are |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
363 going to use the metatable file for any other statistical analyses, |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
364 remember to use the ``Metatable_mod.txt`` one, otherwise the sample |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
365 names might not match! |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
366 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
367 Categories.txt (optional) |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
368 ~~~~~~~~~~~~~~~~~~~~~~~~~ |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
369 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
370 This file is required if you want to get box plots for comparison of |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
371 alpha diversity indices (see **Results** section). The file is a list |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
372 (without header and IN ONE COLUMN) of categories present in the |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
373 Metatable.txt file. THE NAMES YOU ARE USING HAVE TO BE THE SAME AS THE |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
374 ONES USED IN THE METATABLE.TXT. You can create this file in Excel and |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
375 will have to be saved as ``Text(Tab delimited)``. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
376 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
377 EXAMPLE:: |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
378 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
379 Disease |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
380 Gender |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
381 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
382 Metatable and categories files can be uploaded using Get Data as done |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
383 with the fatsq files. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
384 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
385 4. Analysis |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
386 *********** |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
387 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
388 Under **Amplicon_Analysis_Pipeline** |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
389 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
390 * **Title** Name to distinguish between the runs. It will be shown at |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
391 the beginning of each output file name. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
392 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
393 * **Input Metatable.txt file** Select the Metatable.txt file related to |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
394 this analysis |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
395 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
396 * **Input Categories.txt file (Optional)** Select the Categories.txt file |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
397 related to this analysis |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
398 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
399 * **Input FASTQ type** select *Dataset pairs in a collection* and, then, |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
400 the collection of pairs you created earlier. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
401 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
402 * **Forward/Reverse PCR primer sequence** if the PCR primer sequences |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
403 have not been removed from the MiSeq during the fastq creation, they |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
404 have to be removed before the analysis. Insert the PCR primer sequence |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
405 in the corresponding field. DO NOT include any barcode or adapter |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
406 sequence. If the PCR primers have been already trimmed by the MiSeq, |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
407 and you include the sequence in this field, this would lead to an error. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
408 Only include the sequences if still present in the fastq files. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
409 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
410 * **Threshold quality below which reads will be trimmed** Choose the |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
411 Phred score used by Sickle to trim the reads at the 3’ end. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
412 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
413 * **Minimum length to retain a read after trimming** If the read length |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
414 after trimming is shorter than a user defined length, the read, along |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
415 with the corresponding read pair, will be discarded. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
416 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
417 * **Minimum overlap in bp between forward and reverse reads** Choose the |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
418 minimum basepair overlap used by Pandaseq to assemble the reads. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
419 Default is 10. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
420 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
421 * **Minimum length in bp to keep a sequence after overlapping** Choose the |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
422 minimum sequence length used by Pandaseq to keep a sequence after the |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
423 overlapping. This depends on the expected amplicon length. Default is |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
424 380 (used for V3-V4 16S sequencing; expected length ~440bp) |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
425 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
426 * **Pipeline to use for analysis** Choose the pipeline to use for OTU |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
427 clustering and chimera removal. The Galaxy tool supports the ``Vsearch`` |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
428 and ``DADA2`` pipelines. |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
429 |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
430 * **Reference database** Choose between ``GreenGenes``, ``Silva`` or |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
431 ``HOMD`` (Human Oral Microbiome Database) for taxa assignment. |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
432 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
433 Click on **Execute** to start the analysis. |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
434 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
435 5. Results |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
436 ********** |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
437 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
438 Results are entirely generated using QIIME scripts. The results will |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
439 appear in the History panel when the analysis is completed. |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
440 |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
441 The following outputs are captured: |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
442 |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
443 * **Vsearch_tax_OTU_table.biom|DADA2_tax_OTU_table.biom (biom format)** |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
444 The OTU table in BIOM format (http://biom-format.org/) |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
445 |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
446 * **otus.tre** Phylogenetic tree constructed using ``make_phylogeny.py`` |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
447 (fasttree) QIIME script (http://qiime.org/scripts/make_phylogeny.html) |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
448 |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
449 * **Phylum_genus_dist_barcharts_HTML** HTML file with bar charts at |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
450 Phylum, Genus and Species level |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
451 (http://qiime.org/scripts/summarize_taxa.html and |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
452 http://qiime.org/scripts/plot_taxa_summary.html) |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
453 |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
454 * **OTUs_count_file** Summary of OTU counts per sample |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
455 (http://biom-format.org/documentation/summarizing_biom_tables.html) |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
456 |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
457 * **Table_summary_file** Summary of sequences counts per sample |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
458 (http://biom-format.org/documentation/summarizing_biom_tables.html) |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
459 |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
460 * **multiplexed_linearized_dereplicated_mc2_repset_nonchimeras_OTUs.fasta|seqs.fa** |
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
461 Fasta file with OTU sequences (Vsearch|DADA2) |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
462 |
4
86a12d75ebe4
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit 7be61b7ed35ca3deaad68d2eae384c8cd365bcb8
pjbriggs
parents:
3
diff
changeset
|
463 * **Heatmap_PDF** OTU heatmap in PDF format |
0
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
464 (http://qiime.org/1.8.0/scripts/make_otu_heatmap_html.html ) |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
465 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
466 * **Vsearch_beta_diversity_weighted_2D_plots_HTML** PCoA plots in HTML |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
467 format using weighted Unifrac distance measure. Samples are grouped |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
468 by the column names present in the Metatable file. The samples are |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
469 firstly rarefied to the minimum sequencing depth |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
470 (http://qiime.org/scripts/beta_diversity_through_plots.html ) |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
471 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
472 * **Vsearch_beta_diversity_unweighted_2D_plots_HTML** PCoA plots in HTML |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
473 format using Unweighted Unifrac distance measure. Samples are grouped |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
474 by the column names present in the Metatable file. The samples are |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
475 firstly rarefied to the minimum sequencing depth |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
476 (http://qiime.org/scripts/beta_diversity_through_plots.html ) |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
477 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
478 Code availability |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
479 ----------------- |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
480 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
481 **Code is available at** https://github.com/MTutino/Amplicon_analysis |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
482 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
483 Credits |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
484 ------- |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
485 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
486 Pipeline author: Mauro Tutino |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
487 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
488 Galaxy tool: Peter Briggs |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
489 |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
490 ]]></help> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
491 <citations> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
492 <citation type="bibtex"> |
47ec9c6f44b8
planemo upload for repository https://github.com/pjbriggs/Amplicon_analysis-galaxy commit b63924933a03255872077beb4d0fde49d77afa92
pjbriggs
parents:
diff
changeset
|
493 @misc{githubAmplicon_analysis, |
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494 author = {Tutino, Mauro}, |
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495 year = {2017}, |
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496 title = {Amplicon Analysis Pipeline}, |
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497 publisher = {GitHub}, |
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498 journal = {GitHub repository}, |
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499 url = {https://github.com/MTutino/Amplicon_analysis}, |
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500 }</citation> |
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501 </citations> |
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502 </tool> |