Mercurial > repos > pjbriggs > pal_finder
diff README.rst @ 0:3f908e7fff4f draft
Uploaded first version to toolshed.
author | pjbriggs |
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date | Thu, 11 Dec 2014 09:23:24 -0500 |
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children | 771ebe02636f |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.rst Thu Dec 11 09:23:24 2014 -0500 @@ -0,0 +1,106 @@ +pal_finder: find microsatellite repeats and design PCR primers +============================================================== + +Galaxy tool wrapper for the pal_finder microsatellite and PCR primer design script. + +Automated installation +====================== + +Installation via the Galaxy Tool Shed will take of installing the tool wrapper and +the pal_finder and primer3_core programs, and setting the appropriate environment +variables. + +Manual Installation +=================== + +There are two files to install: + +- ``pal_finder_wrapper.xml`` (the Galaxy tool definition) +- ``pal_finder_wrapper.sh`` (the shell script wrapper) + +The suggested location is in a ``tools/pal_finder_wrapper/`` folder. You will then +need to modify the ``tools_conf.xml`` file to tell Galaxy to offer the tool +by adding the line: + + <tool file="pal_finder/pal_finder_wrapper.xml" /> + +You will also need to install the pal_finder and primer3 packages: + +- ``pal_finder`` can be obtained from http://sourceforge.net/projects/palfinder/ +- ``Primer3`` version 2.0.0-alpha (see the pal_finder installation notes) can be + obtained from http://primer3.sourceforge.net/releases.php + +The tool wrapper must be able to locate the pal_finder Perl script, the example +pal_finder config.txt and simple.ref data files, and the primer3_core program - the +locations of these are taken from the following enviroment variables which you will +need to set manually: + +- ``PALFINDER_SCRIPT_DIR``: location of the pal_finder Perl script (defaults to /usr/bin) +- ``PALFINDER_DATA_DIR``: location of the pal_finder data files (specifically config.txt + and simple.ref; defaults to /usr/share/pal_finder_v0.02.04) +- ``PRIMER3_CORE_EXE``: name of the primer3_core program, which should include the + full path if it's not on the Galaxy user's PATH (defaults to primer3_core) + +If you want to run the functional tests, copy the sample test files under +sample test files under Galaxy's ``test-data/`` directory. Then: + + ./run_tests.sh -id microsat_pal_finder + +You will need to have set the environment variables above. + +History +======= + +========== ====================================================================== +Version Changes +---------- ---------------------------------------------------------------------- +0.02.04.1 - Add option to run Graeme Fox's ``pal_finder_filter.pl`` script to + filter and sort the pal_finder output (Illumina input data only). + Update version number to reflect the pal_finder version. +0.0.6 - Allow input data to be either Illumina paired-end data in fastq + format or single-end 454 data in fasta format. +0.0.5 - Allow custom mispriming library to be specified; added + ``tool_dependencies.xml`` file to install pal_finder and primer3 + programs and configure environment for Galaxy automatically. +0.0.4 - Added more custom options for primer3_core for selecting primers on + size, GC and melting temperature criteria. +0.0.3 - Check that pal_finder script & config file, and primer3_core + executable are all available; move PRIMER_MIN_TM parameter to new + "custom" section for primer3 settings +0.0.2 - Updated pal_finder_wrapper.sh to allow locations of pal_finder Perl + script, data files and primer_core3 program to be set via environment + variables +0.0.1 - Initial version +========== ====================================================================== + + +Developers +========== + +This tool is developed on the following GitHub repository: +https://github.com/fls-bioinformatics-core/galaxy-tools/tree/master/pal_finder + +For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball I use +the ``package_pal_finder.sh`` script. + + +Licence (MIT) +============= + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in +all copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN +THE SOFTWARE.