Mercurial > repos > plus91-technologies-pvt-ltd > softsearch
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author | plus91-technologies-pvt-ltd |
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date | Thu, 29 May 2014 07:31:17 -0400 |
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<?xml version="1.0"?> <tool id="SoftSearch" name="SoftSearch"> <description>for structure variation</description> <command> #if $source.index_source=="history" samtools index $bam_file ; samtools faidx $source.history_fasta_file ; $inc | ~/2.4/src/SoftSearch.pl -l $min_length_soft_clip -q $min_map_quality -r $min_depth_soft_clip_loc -m $min_no_discordant_read -s $no_sd_consider_discordant -b $bam_file -f $source.history_fasta_file -o $out_file1 #else samtools index $bam_file ; samtools faidx $source.ref_fasta.fields.path ; $inc | ~/2.4/src/SoftSearch.pl -l $min_length_soft_clip -q $min_map_quality -r $min_depth_soft_clip_loc -m $min_no_discordant_read -s $no_sd_consider_discordant -b $bam_file -f $source.ref_fasta.fields.path -o $out_file1 #end if </command> <inputs> <param name="bam_file" type="data" format="bam" label="BAM Files" /> <conditional name="source"> <param name="index_source" type="select" label="Choose the source for the reference list"> <option value="cached">Locally cached</option> <option value="history">History</option> </param> <when value="history"> <param format="fasta" name="history_fasta_file" type="data" label="Fasta file from history."/> </when> <when value="cached"> <param name="ref_fasta" type="select" > <options from_data_table="fasta_indexes"> <validator type="no_options" message="No Fasta file is available" /> </options> </param> </when> </conditional> <param name="inc" type="hidden" value="n=$RANDOM" /> <param name="min_length_soft_clip" type="integer" value="10" label="-l Minimum length of soft-clipped segment [5]" /> <param name="min_map_quality" type="integer" value="20" label="-q Minimum mapping quality [20]" /> <param name="min_depth_soft_clip_loc" type="integer" value="10" label="-r Minimum depth of soft-clipped reads at position [5]" /> <param name="min_no_discordant_read" type="integer" value="10" label="-m Minimum number of discordant read pairs [5]" /> <param name="no_sd_consider_discordant" type="integer" value="4" label="-s Number of sd away from mean to be considered discordant" /> </inputs> <outputs> <data format="vcf" name="out_file1" /> </outputs> <help> </help> </tool>