# HG changeset patch # User plus91-technologies # Date 1403159383 14400 # Node ID da3c9b9fb9920b3e87b325e6db0a0093ff3f8a05 # Parent 2006814aa6f3489fa306f6836ba28b191a765fc6 Uploaded diff -r 2006814aa6f3 -r da3c9b9fb992 README --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README Thu Jun 19 02:29:43 2014 -0400 @@ -0,0 +1,49 @@ +SoftSearch: + + --SoftSearch is a tools that uses soft-masked reads and discordant read pairs to identify structural variation(SV) in + illumina next-generation sequencing data. + --It is a Integration of Multiple Sequence Features to Identify Breakpoints of Structural Variations. + --Breakpoint is a place or time at which an interruption or change is made. + --SoftSearch requires only two inputs: a BAM file of aligned reads and fasta file of the genome to which the reads were aligned. + + Google Code Link: + + https://code.google.com/p/softsearch/ + +------------------------------------------------------------------------------------------------------------------ +Pre-Requirement: + + -Perl + -Bedtools + -SamTools + Must have an entry in environment variable + +------------------------------------------------------------------------------------------------------------------ +How To Selecte fasta file from Database (Additional Configuration): + + --Select fasta_indexes.loc file for that go to shed_tools/toolshed.g2.bx.psu.edu/repos/plus91-technologies/softsearch/ + 8578d978014c/softsearch/tool-data/ + + --open the fasta_indexes.loc file and make changes like + + + + eg: hg19full hg19 Human (Homo sapiens): hg19 Full /depot/data2/galaxy/hg19/sam/hg19full.fa + + --open shed_tool_data_table_conf.xml file and add following. + + + + value, dbkey, name, path + +
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+ +------------------------------------------------------------------------------------------------------------------ +Authors: + + ResearchDx + http://researchdx.com + + Plus91 Technologies Private Limited + http://www.plus91.in/