# HG changeset patch
# User plus91-technologies
# Date 1401361555 14400
# Node ID ac5bcbd14b51c1f17947a62043a36af455ba14b3
# Parent  ddc587fed3875fbd3a55dbd963e199f3596e1c23
Deleted selected files

diff -r ddc587fed387 -r ac5bcbd14b51 SoftSearch.pl
--- a/SoftSearch.pl	Thu May 29 06:50:23 2014 -0400
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,1192 +0,0 @@
-#!/usr/bin/perl
-
-####
-#### Usage: SoftSearch.pl [-lqrmsd] -b <BAM> -f <Genome.fa> -sam <samtools path> -bed <bedtools path>
-#### Created 1-30-2012 by Steven Hart, PhD
-#### hart.steven@mayo.edu
-#### Required bedtools & samtools to be in path
-
-
-use lib "/data2/bsi/reference/softsearch/lib" ;
-
-use Getopt::Long;
-use strict;
-use warnings;
-#use Data::Dumper;
-use LevD;
-use File::Basename;
-
-my ($INPUT_BAM,$INPUT_FASTA,$OUTPUT_FILE,$minSoft,$minSoftReads,$dist_To_Soft,$bedtools,$samtools);
-my ($minRP, $temp_output, $num_sd, $MapQ, $chrom, $unmated_pairs, $minBQ, $pair_only, $disable_RP_only);
-my ($levD_local_threshold, $levD_distl_threshold,$pe_upper_limit,$high_qual,$sv_only,$blacklist,$genome_file,$verbose);
-
-my $cmd = "";
-
-#Declare variables
-GetOptions(
-	'b=s' => \$INPUT_BAM,
-	'f=s' => \$INPUT_FASTA,
-	'o:s' => \$OUTPUT_FILE,
-	'm:i' => \$minRP,
-	'l:i' => \$minSoft,
-	'r:i' => \$minSoftReads,
-	't:i' => \$temp_output,
-	's:s' => \$num_sd,
-	'd:i' => \$dist_To_Soft,
-	'q:i' => \$MapQ,
-	'c:s' => \$chrom,
-	'u:s' => \$unmated_pairs,
-	'x:s' => \$minBQ,
-	'p' => \$pair_only,
-	'g' => \$disable_RP_only,
-	'j:s' => \$levD_local_threshold,
-	'k:s' => \$levD_distl_threshold,
-        'a:s' => \$pe_upper_limit,
-        'e:s' => \$high_qual,
-	'L' => \$sv_only,
-	'v' => \$verbose, 
-	'blacklist:s' => \$blacklist,
-	'genome_file:s' => \$genome_file,
-	"help|h|?"	=> \&usage);
-
-unless($sv_only){$sv_only=""};
-if(defined($INPUT_BAM)){$INPUT_BAM=$INPUT_BAM} else {print usage();die "Where is the BAM file?\n\n"}
-if(defined($INPUT_FASTA)){$INPUT_FASTA=$INPUT_FASTA} else {print usage();die "Where is the fasta file?\n\n"}
-my ($fn,$pathname) = fileparse($INPUT_BAM,".bam");
-my $index=`ls $pathname/$fn*bai|head -1`;
-#my $index =`ls \${INPUT_BAM%.bam}*bai`;
-#print "INDEX=$index\n";
-if(!$index){die "\n\nERROR: you need index your BAM file\n\n"}
-
-### get current time
-print "Start Time : " . &spGetCurDateTime() . "\n";
-my $now = time;
-
-#if(defined($OUTPUT_FILE)){$OUTPUT_FILE=$OUTPUT_FILE} else {$OUTPUT_FILE="output.vcf"; print "\nNo outfile specified.  Using output.vcf as default\n\n"}
-if(defined($minSoft)){$minSoft=$minSoft} else {$minSoft=5}
-if(defined($minRP)){$minRP=$minRP} else {$minRP=5}
-if(defined($minSoftReads)){$minSoftReads=$minSoftReads} else {$minSoftReads=5}
-if(defined($dist_To_Soft)){$dist_To_Soft=$dist_To_Soft} else {$dist_To_Soft=300}
-if(defined($num_sd)){$num_sd=$num_sd} else {$num_sd=6}
-if(defined($MapQ)){$MapQ=$MapQ} else {$MapQ=20}
-
-unless (defined $pe_upper_limit) { $pe_upper_limit = 10000; }
-unless (defined $levD_local_threshold) { $levD_local_threshold = 0.05; }
-unless (defined $levD_distl_threshold) { $levD_distl_threshold = 0.05; }
-#Get sample name if available
-my $SAMPLE_NAME="";
-my $OUTNAME ="";
-$SAMPLE_NAME=`samtools view -f2 -H $INPUT_BAM|awk '{if(\$1~/^\@RG/){sub("ID:","",\$2);name=\$2;print name}}'|head -1`;
-$SAMPLE_NAME=~s/\n//g;
-if (!$OUTPUT_FILE){
-	if($SAMPLE_NAME ne ""){$OUTNAME=$SAMPLE_NAME.".vcf"}
-	else {$OUTNAME="output.vcf"}
-}
-else{$OUTNAME=$OUTPUT_FILE}
-
-print "Writing results to $OUTNAME\n";
-
-
-##Make sure if submitting on SGE, to prepned the "chr".  Not all referecne FAST files require "chr", so we shouldn't force the issue.
-if(!defined($chrom)){$chrom=""}
-if(!defined($unmated_pairs)){$unmated_pairs=0}
-
-my $badQualValue=chr($MapQ);
-if(defined($minBQ)){ $badQualValue=chr($minBQ); }
-
-if($badQualValue  eq "#"){$badQualValue="\#"}
-
-# adding and cheking for samtools and bedtools in the PATh
-## check for bedtools and samtools in the path
-$bedtools=`which intersectBed` ;
-if(!defined($bedtools)){die "\nError:\n\tno bedtools. Please install bedtools and add to the path\n";}
-#$samtools=`samtools 2>&1`;
-$samtools=`which samtools`;
-if($samtools !~ /(samtools)/i){die "\nError:\n\tno samtools. Please install samtools and add to the path\n";}
-
-print "Usage = SoftSearch.pl -l $minSoft -q $MapQ -r $minSoftReads -d $dist_To_Soft -m $minRP -s $num_sd -c $chrom -b $INPUT_BAM -f $INPUT_FASTA -o $OUTNAME \n\n";
-sub usage {
-	print "\nusage: SoftSearch.pl [-cqlrmsd] -b <BAM> -f <Genome.fa> \n";
-	print "\t-q\t\tMinimum mapping quality [20]\n";
-	print "\t-l\t\tMinimum length of soft-clipped segment [5]\n";
-	print "\t-r\t\tMinimum depth of soft-clipped reads at position [5]\n";
-	print "\t-m\t\tMinimum number of discordant read pairs [5]\n";
-	print "\t-s\t\tNumber of sd away from mean to be considered discordant [6]\n";
-	print "\t-u\t\tNumber of unmated pairs [0]\n";
-	print "\t-d\t\tMax distance between soft-clipped segments and discordant read pairs [300]\n";
-	print "\t-o\t\tOutput file name [output.vcf]\n";
-	print "\t-t\t\tPrint temp files for debugging [no|yes]\n";
-	print "\t-c\t\tuse only this chrom or chr:pos1-pos2\n";
-	print "\t-p\t\tuse paired-end mode only. In other words, don't try to find soft-clipping events!\n";
-	print "\t-g\t\tEnable paired-only seach. This will look for discordant read pairs even without soft clips.\n";
-        print "\t-a\t\tset the minimum distance for a discordant read pair without soft-clipping info [10000]\n";
-        print "\t-L\t\tFlag to print out even small deletions (low quality)\n";
-        print "\t-e\t\tdisable strict quality filtering of base qualities in soft-clipped reads [no]\n";
-        print "\t-blacklist\tareas of the genome to skip calling.  Requires -genome_file\n";
-        print "\t-genome_file\ttab seperated value of chromosome name and length.  Only used with -blacklist option\n\n";
-
-	exit 1;
-	}
-
-
-#############################################################
-# create temporary variable name
-#############################################################
-srand (time ^ $$ ^ unpack "%L*", `ps axww | gzip -f`);
-our $random_name=join "", map { ("a".."z")[rand 26] } 1..8;
-
-#############################################################
-## create green list
-##############################################################
-#
-my $new_blacklist="";
-if($blacklist){
-        if(!$genome_file){die "if using a blacklist, you must also specify the location of a genome_file
-        The format of the genome_file should be
-                chrom   size
-                chr1    249250621
-                chr2    243199373
-                ...
-
-        If using hg19, you can ge the genome file by
-                mysql --user=genome --host=genome-mysql.cse.ucsc.edu -A -e \"select chrom, size from hg19.chromInfo\"  > hg19.genome";}
-        
-	$cmd=join("","complementBed -i $blacklist -g $genome_file >",$random_name,".bed") ;
-	system ($cmd);
-	$new_blacklist=join(""," -L ",$random_name,".bed ");
-	}
-
-if($verbose){print "CMD=$cmd\nBlacklist is $new_blacklist\n";}
-
-
-
-
-
-#############################################################
-# Calcualte insert size distribution of properly mated reads
-#############################################################
-
-#Change for compatability with other operating systems
-#my $metrics=`samtools view -q $MapQ -f2 $INPUT_BAM $chrom|cut -f9|head -10000|awk '{if (\$1<0){\$1=-\$1}else{\$1=\$1} sum+=\$1; sumsq+=\$1*\$1} END {print sum/NR, sqrt(sumsq/NR - (sum/NR)**2)}'`;
-
-my $metrics=`samtools view -q $MapQ -f2 $INPUT_BAM $chrom|cut -f9|head -10000|awk '{if (\$1<0){\$1=-\$1}else{\$1=\$1} sum+=\$1; sumsq+=\$1*\$1} END {print sum/NR, sqrt(sumsq/NR - (sum/NR)^2)}'`;
-#my ($mean,$stdev)=split(/ /,$metrics);
-my ($mean,$stdev)=split(/\s/,$metrics);
-$stdev=~s/\n//;
-my $upper_limit=int($mean+($num_sd*$stdev));
-my $lower_limit=int($mean-($num_sd*$stdev));
-die if (!$mean);
-print qq{The mean insert size is $mean +/- $stdev (sd)
-The upper limit = $upper_limit
-The lower limit = $lower_limit\n
-};
-if($lower_limit<0){
-	print "Warning!! Given this insert size distribution, we can not call small indels.  No other data will be affected\n";
-	$lower_limit=1;
-}
-my $tmp_name=join ("",$random_name,".tmp.bam");
-my $random_file_sc = "";
-my $command = "";
-
-#############################################################
-# Make sam file that has soft clipped reads
-#############################################################
-#give file a name
-if(!defined($pair_only)){
-	$random_file_sc=join ("",$random_name,".sc.sam");
-	$command=join ("","samtools view -q $MapQ -F 1024 $INPUT_BAM $chrom $new_blacklist| awk '{OFS=\"\\t\"}{c=0;if(\$6~/S/){++c};if(c == 1){print}}' | perl -ane '\$TR=(\@F[10]=~tr/\#//);if(\$TR<2){print}' > ", $random_file_sc);
-
-	print "Making SAM file of soft-clipped reads\n";
-if($verbose){	print "$command\n";}
-	system("$command");
-
-	#############################################################
-	# Find areas that have deep enough soft-clip coverage
-	print "Identifying soft-clipped regions that are at least $minSoft bp long \n";
-	open (FILE,"$random_file_sc")||die "Can't open soft-clipped sam file $random_file_sc\n";
-
-	my $tmpfile=join("",$random_file_sc,".sc.passfilter");
-	open (OUT,">$tmpfile")||die "Can't write files here!\n";
-
-	while(<FILE>){
-		@_ = split(/\t/, $_);
-		#### parse CIGAR string and create a hash of array of each operation
-		my @CIGAR = split(/([0-9]+[SMIDNHXP])/, $_[5]);
-		my $hash;
-		map { push(@{$hash->{$2}}, $1) if (/(\d+)([SMIDNHXP])/) } @CIGAR;
-
-		#for ($i=0; $i<=$#softclip_pos; $i++)	{
-		foreach my $softclip (@{$hash->{S}}) {
-			#if	($CIGAR[$softclip_pos[$i]] > $minSoft){
-			if	($softclip > $minSoft){
-				###############Make sure base qualities don't have more than 2 bad marks
-				my $qual=$_[10];
-				my $TR=($qual=~tr/$badQualValue//);
-				if($badQualValue eq "#"){ $TR=($qual=~tr/\#//); }
-				#Skip the soft clip if there is more than 2 bad qual values
-				#next if($TR > 2);
-#				if (!$high_qual){next if($TR > 2);}
-				print OUT;
-				last;
-			}
-		}
-	}
-	close FILE;
-	close OUT;
-
-	$command=join(" ","mv",$tmpfile,$random_file_sc);
-if($verbose){	print "$command\n";}
-	system("$command");
-}
-
-#########################################################
-#Stack up SoftClips
-#########################################################
-my $random_file=join("",$random_name,".sc.direction.bed");
-if(!defined($pair_only)){
-        open (FILE,"$random_file_sc")|| die "Can't open sam file\n";
-        #$random_file=join("",$random_name,".sc.direction");
-
-        print "Calling sides of soft-clips\n";
-        #\nTMPOUT=$random_file\tINPUT=$random_file_sc\n\n";
-        open (TMPOUT,">$random_file")|| die "Can't create tmp file\n";
-
-        while (<FILE>){
-                @_ = split(/\t/, $_);
-                #### parse CIGAR string and create a hash of array of each operation
-                my @CIGAR = split(/([0-9]+[SMIDNHXP])/, $_[5]);
-                my $hash;
-                map { push(@{$hash->{$2}}, $1) if (/(\d+)([SMIDNHXP])/) } @CIGAR;
-
-                #### next if softclips on each end
-                next if ($_[5] =~ /^[0-9]+S.*S$/);
-
-                #### next softclip occurs in the middle
-                next if ($_[5] =~ /^[0-9]+[^S][0-9].*S.+$/);
-
-                my $softclip = $hash->{S}[0];
-
-                my $end1 = 0;
-                my $end2 = 0;
-                my $softBases = "";
-		my $right_corrected="";my $left_corrected="";
-
-        if ($softclip > $minSoft) {
-		
-                        ####If the soft clip occurs at end of read and its on the minus strand, then it's a right clip
-                        if ($_[5] =~ /^.*S$/) {
-                                $end1=$_[3]+length($_[9])-$softclip-1;
-                                $end2=$end1+1;
-next if ($end1<0);
-                                #RIGHT clip on Minus
-                                $softBases=substr($_[9], length($_[9])-$softclip, length($_[9]));
-                                #Right clips don't always get clipped correctly, so fix that
-                                # Check to see if sc base matches ref
-                                $right_corrected=baseCheck($_[2],$end2,"right",$softBases);
-                               print TMPOUT "$right_corrected\n"
-
-                        } else {
-                                #### Begins with S (left clip)
-                                $end1=$_[3]-$softclip;
-next if ($end1<0);
-
-                                $softBases=substr($_[9], 0,$softclip);#print "TMP=$softBases\n";
-        			$left_corrected=baseCheck($_[2],$end1,"left",$softBases);
-if(!$left_corrected){print "baseCheck($_[2],$end1,left,$softBases)\n";next}
-                               print TMPOUT "$left_corrected\n";
-#print "\nSEQ=$_[9]\t\n";
-
-                        }
-        }
-  }
-close FILE;
-close TMPOUT;
-}
-sub baseCheck{
-        my ($chrom,$pos,$direction,$softBases)=@_;
-        #skip if position is less than 0, which is caused by MT DNA
-        return if ($pos<0);
-        my $exit="";
-
-        while(!$exit){
-        if($direction=~/right/){
-                        my $refBase=getSeq($chrom,$pos,$INPUT_FASTA);
-                        my $softBase=substr($softBases,0,1);
-                        if ($softBase !~ /$refBase/){
-                                my $value=join("\t",$chrom,$pos,$pos+1,join("|",$softBases,$direction));
-                                $exit="STOP";
-                                return $value;
-                        }
-                        else{
-                                $pos=$pos+1;
-                                $softBases=substr($softBases, 1,length($softBases));
-                        }
-         }
-        else{
-                        my $refBase=getSeq($chrom,$pos+1,$INPUT_FASTA);
-                        my $softBase=substr($softBases,-1,1);
-                        if ($softBase !~ /$refBase/){
-                                $pos=$pos-1+length($softBases);
-                                my $value=join("\t",$chrom,$pos-1,$pos,join("|",$softBases,$direction));
-                                $exit="STOP";
-                                return $value;
-                        }
-                        else{
-                                $pos=$pos-1;
-                                $softBases=substr($softBases, 0, -1);
-                                #print "Trying again $softBases\n";
-                       }
-
-        }
-
-}
-}
-#Remove SAM files to conserve space
-unlink($random_file_sc);
-
-
-my $random_file_disc="$INPUT_BAM";
-###
-#
-######################################################
-# Transform Read pair groups into softclip equivalents
-######################################################
-#
-#
-#
-my $v="";
-#if($disable_RP_only){
-print "Running Bam2pair.pl\n";
-print "Looking for discordant read pairs without requiring soft-clipping information\n";
-	use FindBin qw($Bin);
-	my $path=$Bin;
-#	print"\n\nPATH=$path\n\n";
-if($verbose){$v="-v"}
-	my $tmp_out=join("",$random_name,"RP.out");
-	$command=join("","perl ",$path,"/Bam2pair.pl -b $random_file_disc  -o $tmp_out -isize $pe_upper_limit -winsize $dist_To_Soft -min $minRP -chrom $chrom -prefix $random_name -q $MapQ -blacklist $random_name.bed $v");
-if($verbose){	print "$command\n"};
-	system("$command");
-	$command=join("","perl -ane '\$end1=\@F[1];\$end2=\@F[3];print join(\"\\t\",\@F[0..1],\$end1,\"unknown|left\");print \"\\n\";print join(\"\\t\",\@F[2..3],\$end2,\"unknown|left\");print \"\\n\"' ", $tmp_out," >> ",$random_file);
-if($verbose){print "$command\n"};
-	system($command);
-	unlink($tmp_out);
-#}
-#
-
-
-######################################################
-unlink("$random_file","$tmp_name","$random_file","$index","$random_name","$new_blacklist") if (-z $random_file || ! -e $random_file ) ;
-if (-z $random_file || ! -e $random_file){
-	print "Softclipped file is empty($random_file).\nNo soft clipping found using desired paramters\n\n";
-	open (OUT,">$OUTNAME")||die "Can't write files here!\n";
-        &print_header();
-        close OUT;
-        exit;
-	}
-
-
-#############################################################
-#  Make sure there are enough soft-clippped supporting reads
-#############################################################
-my $outfile=join("",$random_file,".sc.merge.bed");
-#sortbed -i .sc.direction | mergeBed -nms -d 25 -i stdin > .sc.merge.bed
-$command=join(" ","sortBed -i",$random_file," | mergeBed  -nms -i stdin","|egrep \";|,\"","|awk '{OFS=\"\t\"}(NF==4)'",">",$outfile);
-
-print "$command\n" if ($verbose);
-system("$command");
-
-if (-z $outfile || ! -e $outfile){
-	unlink("$tmp_name","$random_file","$outfile","$index","$random_name","$new_blacklist"); 
-	print "mergeBed file is empty.\nNo strucutral variants found\n\n" ;
-        open (OUT,">$OUTNAME")||die "Can't write files here!\n";
-        &print_header();
-        close OUT;
-        exit;
-}
-
-print "completed mergeBed\n";
-
-###############################################################
-# If left and right are on the same line, make into 2 lines
-###############################################################
-open (INFILE,$outfile)||die "couldn't open temp file : $. \n\n";
-my $tmp2=join("",$random_name,".sc.fixed.merge.bed");
-#print "INFILE=$outfile\tOUTFILE=$tmp2\n\n";
-#INPUT FORMAT=chr9\t131467\t131473\tATGCTTATTAAAA|left;TTATTAAAAGCATA|left
-open (OUTFILE,">$tmp2")||die "couldn't create temp file : $. \n\n";
-while(<INFILE>){
-	chomp $_;
-	my $l = $_;
-
-	my @a = split(/\t/, $l);
-	my $info = $a[3];
-	my @info_arr = split(/\;/, $info);
-	my @left_arr=();
-	my @right_arr=();
-	@left_arr = grep(/left/, @info_arr);
-	@right_arr = grep(/right/, @info_arr);
-
-	#New
-	my $left = join(";", @left_arr);
-	my $right = join(";", @right_arr);
-	$info = join(";", @info_arr);
-
-	if((@left_arr) && (@right_arr)){
-		print OUTFILE "$a[0]\t$a[1]\t$a[2]\t$left\n$a[0]\t$a[1]\t$a[2]\t$right\n";
-	} else{
-		my $all=join("\t",@a[0..2],$info);
-		print OUTFILE "$all\n";
-	}
-}
-
-# make sure output file name is $outfile
-$command=join(" ","sed -e '/ /s//\t/g'", $tmp2,"|awk 'BEGIN{OFS=\"\\t\"}(NF==4)'", "|perl -pne 's/ /\t/g'>",$outfile);
-system("$command");
-if($verbose){print "$command\n"};
-unlink("$tmp_name","$random_file","$tmp2","$outfile","$index","random_name","$new_blacklist") if (-z $outfile || ! -e $outfile) ;
- if (-z $outfile || ! -e $outfile){
-	print "Fixed mergeBed file is empty($outfile).\nNo strucutral variants found\n\n";
-        open (OUT,">$OUTNAME")||die "Can't write files here!\n";
-        &print_header();
-        close OUT;
-        exit;
-}
-
-print "completed fixing mergeBed\n\n";
-
-###############################################################
-# Seperate directions of soft clips
-###############################################################
-my $left_sc = join("", "left", $tmp2);
-my $right_sc = join("", "right", $tmp2);
-use FindBin qw($Bin);
-#my $path=$Bin;
-
-$command=join("","grep left ", $tmp2, " |sed -e '/left /s//left\;/g' | sed -e '/ /s//\t/g'|perl ".$path."/direction_filter.pl - >",$left_sc);
-system("$command");
-#print "$command\n";
-$command=join("","grep right ", $tmp2, " |sed -e '/right /s//right\;/g' | sed -e '/ /s//\t/g'|perl ".$path."/direction_filter.pl - >",$right_sc);
-#$command=join(" ","grep right ", $tmp2, " |sed -e '/right /s//right\;/g' | sed -e '/ /s//\t/g' >",$right_sc);
-system("$command");
-#print "$command\n";
-#die "CHECK $right_sc\n";
-
-###############################################################
-# Count the number and identify directions of soft clips
-###############################################################
-print "Count the number and identify directions of soft clips\n";
-#print "looking in $outfile\n";
-$outfile=join("",$random_name,".sc.fixed.merge.bed");
-
-open (INFILE,$outfile)||die "couldn't open temp file\n\n";
-my $tmp3 = join("", $random_file, "predSV");
-open (OUTFILE, ">$tmp3")||die "couldn't create temp file\n\n";
-while(<INFILE>){
-chomp;
-	@_=split(/\t/,$_);
-	my $count=tr/\;//;$count+=tr/\,//;
-	$count=$count+1;
-	my $left=0;
-	my $right=0;
-
-	while ($_ =~ /left/g) { $left++ } # count number of right clips
-	while ($_ =~ /right/g) { $right++ } # count number of left clips
-
-	###############################################################
-	if ($count >= $minSoftReads){
-		####get longets soft-clipped read
-		my @clips=split(/\;|,|\|/,$_[3]);
-
-		my ($max, $temp, $temp2, $temp3, $dir, $maxSclip) = (0) x 6;
-		for (my $i=0; $i<$count; $i++) {
-			my $plus1=$i+1;
-			$temp=length($clips[$i]);
-			$temp2=$clips[$plus1];
-			$temp3=$clips[$i];
-
-			if ($temp > $max){
-				$maxSclip=$temp3;
-				$max =$temp;
-				$dir=$temp2;
-			} else {
-				$max=$max;
-				$dir=$dir;
-				$maxSclip=$maxSclip;
-			}
-			$i++;
-		}
-		my $order2 = join("|", $left, $right);
-        #print join ("\t",@_[0..2],$maxSclip,$max,$dir,$count,$order2) . "\n";
-		print OUTFILE join ("\t",@_[0..2],$maxSclip,$max,$dir,$count,$order2) . "\n";
-	} elsif($_=~/unknown/){
-	print OUTFILE join ("\t",@_[0..2],"NA","NA","left","NA","NA|NA") . "\n";
-        print OUTFILE join ("\t",@_[0..2],"NA","NA","right","NA","NA|NA") . "\n";
-	}
-	####Format is Chrom,start, end,longest Soft-clip,length of longest Soft-clip, direction of longest soft-clip,#supporting softclips,#right Sclips|#left Sclips
-}
-close INFILE;
-close OUTFILE;
-
-unlink("$tmp2","$tmp_name","$random_file","$tmp3","$outfile","$index","$random_name","$right_sc","$left_sc","$new_blacklist") if (-z $tmp3 || !-e $tmp3) ;
-
- if (-z $tmp3 || !-e $tmp3){
-	print "No structural variants found while Counting the number and identify directions of soft clips.\n" ;
-
-	open (OUT,">$OUTNAME")||die "Can't write files here!\n";
-	&print_header();
-	close OUT;
-	exit;
-
-}
-
-print "Done counting Softclipped reads\n";
-###############################################################
-#### Print header information
-###############################################################
-open (OUT,">$OUTNAME")||die "Can't write files here!\n";
-&print_header();
-close OUT;
-
-###############################################################
-###############################################################
-#### DO the bulk of the work
-###############################################################
-use List::Util qw(min max);
-open (FILE,"$tmp3")|| die "Can't open file\n";
-open (OUT,">>$OUTNAME")|| die "Can't open file\n";
-
-#print "\nusing $tmp3 and writing to $OUTPUT_FILE \n";
-while (<FILE>){
-	#If left clip {+- or -- or -+ }{+- are uninformative b/c they go upstream}
-	#If right clip {++ or -- or +-}
-	chomp $_;
-	my $line = $_;
-	my @info = split(/\t/, $_);
-
-	if($info[5] eq "left") {
-		bulk_work("left", $line, $random_file_disc);
-
-	} elsif ($info[5] eq "right") {
-		bulk_work("right", $line, $random_file_disc);
-	}
-#if($. ==6){print "THIS IS LINE 6\n$_\n";die}
-print "Completed line $.\n" if ($verbose);
-}
-close FILE;
-close OUT;
-
-###############################################################################
-###############################################################################
-#### Delete temp files
-my $meregedBed=join("",$random_name,".sc.direction.bed.sc.merge.bed");
-
-if(defined($temp_output)){$temp_output=$temp_output} else {$temp_output="no"}
-
-if ($temp_output eq "no"){
-	unlink("$tmp_name","$random_file","$tmp2",,"$tmp3","$outfile","$index","$random_name","$right_sc","$left_sc","$meregedBed","$random_name.bed");
-}
-####Sort VCF
-my $tmp=join(".",$random_name,"tmp");
-#Get header
-$cmd="grep \"#\" $OUTNAME > $tmp";
-system($cmd);
-#sort results
-$cmd="grep -v \"#\" $OUTNAME|perl -pne 's/chr//'|sort -k1,1n -k2,2n|perl -ne 'print \"chr\".\$_' >>$tmp";
-system($cmd);
-$cmd="mv $tmp $OUTNAME";
-system($cmd);
-#remove entries next to each other
-
-
-
-
-#############################################################
-##May not need this anymore since filtering on left and right
-#############################################################
-#my $tmpout=$OUTNAME;
-#$tmpout.=".tmp";
-#use FindBin qw($Bin);
-##my $path=$Bin;
-#$command="perl ".$path."/Extract_nSC.pl $OUTNAME -q nSC > $tmpout";
-##print "Command=$command\n";
-#system($command);
-#$command="perl ".$path."/reduce_redundancy.pl $tmpout $upper_limit |cut -f1-10 > $OUTNAME";
-##print "$command\n";
-#system($command);
-#system("rm $tmpout");
-########################################################
-
-
-
-
-print "Analysis Completed\n\nYou did it!!!\n";
-print "Finish Time : " . &spGetCurDateTime() . "\n";
-$now = time - $now;
-printf("\n\nTotal running time: %02d:%02d:%02d\n\n", int($now / 3600), int(($now % 3600) / 60),
-int($now % 60));
-
-exit;
-
-###############################################################################
-sub rev_comp {
-  my $dna = shift;
-  my $revcomp = reverse($dna);
-  $revcomp =~ tr/ACGTacgt/TGCAtgca/;
-
-  return $revcomp;
-}
-
-
-###############################################################################
-#### to get reference base
-sub getSeq{
-	my ($chr,$pos,$fasta)=@_;
-	#don't require chr
-	#if($chr !~ /^chr/){die "$chr is not correct\n";}
-#	die "$pos is not a number\n" if ($pos <0);
-my @result=();
-        if ($pos <0){print "$pos is not a valid position (likely caused by circular MT chromosome)\n";return;}
-
-	@result = `samtools faidx $fasta $chr:$pos-$pos`;
-	if($result[1]){chomp($result[1]);
-	return uc($result[1]);
-	}
-	return("NA");
-	#### after return will not be printed
-	####print "RESULTS=@result\n";
-}
-
-sub getBases{
-        my ($chr,$pos1,$pos2,$fasta)=@_;
-        #don't require chr
-        #if($chr !~ /^chr/){die "$chr is not correct\n";}
-my @result=();
-        if ($pos1 <0){print "$pos1 is not a valid position (likely caused by circular MT chromosome)\n";return;};
-
-        @result = `samtools faidx $fasta $chr:$pos1-$pos2`;
-	if(!$result[1]){$result[1]="NA"};
-        chomp($result[1]);
-        return uc($result[1]);
-
-        #### after return will not be printed
-        ####print "RESULTS=@result\n";
-}
-###############################################################################
-#### to get time
-sub spGetCurDateTime {
-	my ($sec, $min, $hour, $mday, $mon, $year) = localtime();
-	my $curDateTime = sprintf "%4d-%02d-%02d %02d:%02d:%02d",
-	$year+1900, $mon+1, $mday, $hour, $min, $sec;
-	return ($curDateTime);
-}
-
-
-###############################################################################
-#### print header
-sub print_header {
-	my $date=&spGetCurDateTime();
-	my $header = qq{##fileformat=VCFv4.1
-##fileDate=$date
-##source=SoftSearch.pl
-##reference=$INPUT_FASTA
-##Usage= SoftSearch.pl -l $minSoft -q $MapQ -r $minSoftReads -d $dist_To_Soft -m $minRP -u $unmated_pairs -s $num_sd -b $INPUT_BAM -f $INPUT_FASTA -o $OUTNAME
-##INFO=<ID=SVTYPE,Number=1,Type=String,Description="Type of structural variant">
-##INFO=<ID=EVENT,Number=1,Type=String,Description="ID of event associated to breakend">
-##INFO=<ID=MATEID,Number=.,Type=String,Description="ID of mate breakends">
-##INFO=<ID=ISIZE,Number=.,Type=String,Description="Size of the SV">
-##INFO=<ID=END,Number=1,Type=Integer,Description="End position of the variant described in this record">
-##FORMAT=<ID=lSC,Number=1,Type=Integer,Description="Length of the longest soft clips supporting the BND">
-##FORMAT=<ID=nSC,Number=1,Type=Integer,Description="Number of supporting soft-clips\">
-##FORMAT=<ID=uRP,Number=1,Type=Integer,Description="Number of unmated read pairs nearby Soft-Clips">
-##FORMAT=<ID=levD_local,Number=1,Type=Float,Description="Levenstein distance between soft-clipped bases and the area around the original soft-clipped site">
-##FORMAT=<ID=levD_distl,Number=1,Type=Float,Description="Levenstein distance between the soft-clipped bases and mate location">
-##FORMAT=<ID=CTX,Number=1,Type=Integer,Description="Number of chromosomal translocations">
-##FORMAT=<ID=DEL,Number=1,Type=Integer,Description="Number of reads supporting Large Deletions">
-##FORMAT=<ID=INS,Number=1,Type=Integer,Description="Number of reads supporting Large insertions">
-##FORMAT=<ID=NOV_INS,Number=1,Type=Integer,Description="Number of reads supporting novel sequence insertion">
-##FORMAT=<ID=TDUP,Number=1,Type=Integer,Description="Number of reads supporting a tandem duplication">
-##FORMAT=<ID=INV,Number=1,Type=Integer,Description="Number of reads supporting inversions">
-##FORMAT=<ID=sDEL,Number=1,Type=Integer,Description="Number of reads supporting novel sequence insertion">
-##INFO=<ID=NO_MATE_SC,Number=1,Type=Flag,Description="When there is no softclipping of the mate read location, an appromiate position is used">
-##FORMAT=<ID=GT,Number=1,Type=String,Description="Dummy value for maintaining VCF-Spec">
-#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO\tFORMAT\t$SAMPLE_NAME\n};
-
-	print OUT $header;
-}
-
-
-###############################################################################
-sub bulk_work {
-print "#####################################@_\n" if ($verbose);
-	my ($side, $line, $file) = @_;
-	my $local_levD = 0;
-	my $distl_levD = 0;
-
-	#my @info = split(/\t/, $line);
-	my @plus_Reads = split(/\t/, $line);
-	$plus_Reads[7] =~ s/\n//g;
-
-	#### softclip length and softclip size.
-	my $lSC = $plus_Reads[4];
-	my $nSC = $plus_Reads[6];
-
-
-	#Get all types of compatible reads
-	#Get improperly paired reads (@ max distance)
-
-	#### default value for left SIDE.
-	#If left-clip, then look downstream for match of softclipped reads to define a deletion, but look for DRPs upstream
-	my $sv_type = "SVTYPE=BND";
-	my $start_local=0; my $end_local=0;my $target_local="";my $target_drp="";my $start_drp="";my $end_drp="";
-	if ($side =~ /left/) {
-		$start_local = $plus_Reads[1]-$dist_To_Soft;
-		$end_local = $plus_Reads[2];
-                $start_drp = $plus_Reads[1];
-                $end_drp = $plus_Reads[1]+$dist_To_Soft;
-	
-	}
-	else{                
-                $start_local = $plus_Reads[1];
-                $end_local = $plus_Reads[1]+$dist_To_Soft;
-                $start_drp = $plus_Reads[1]-$dist_To_Soft;
-                $end_drp = $plus_Reads[1];
-        }
-	
-	$target_local=join("", $plus_Reads[0], ":", $start_local, "-", $end_local);
-	$target_drp=join("", $plus_Reads[0], ":", $start_drp, "-", $end_drp);
-	my $num_unmapped_pairs="";
-	if ($side =~ /right/) {
-		$num_unmapped_pairs=`samtools view $new_blacklist -q $MapQ -f8 -F 1536 -c $INPUT_BAM $target_drp`;
-	} else {
-        $num_unmapped_pairs=`samtools view $new_blacklist -q $MapQ -f24 -F 1536 -c $INPUT_BAM $target_drp`;
-	}
-if($verbose){print "samtools view $new_blacklist -q $MapQ -f24 -F 1536 -c $INPUT_BAM $target_drp\n";}
-
-	$num_unmapped_pairs=~s/\n//;
-if($verbose){print "NUM UNMAPPED PAIRS= $num_unmapped_pairs\n";}
-	my $REF1_base = "";
-	my $REF2_base = "";
-	my $INFO_1 = "";
-	my $INFO_2 = "";
-	my $ALT_1 = "";
-	my $ALT_2 = "";
-	my $isize = 0;
-	my $QUAL = "";
-	my $FORMAT = "GT:";
-
-	#### get 8 bit rand id
-	my $BND1_name = join "", map { ("a".."z")[rand 26] } 1..8;
-	my $BND2_name = join "", map { ("a".."z")[rand 26] } 1..8;
-	$BND1_name=join "_","BND",$BND1_name;
-	$BND2_name=join "_","BND",$BND2_name;
-
-	my $counts = {CTX => 0, DEL => 0, INS => 0, INV => 0, TDUP => 0, NOV_INS => 0 };
-	my $event_mate_info = {CTX => "", DEL => "", INS => "", INV => "", TDUP => "", NOV_INS => "" };
-
-	#### get mate pair info and counts per event
-	foreach my $e (sort keys %{$counts}) {
-		my $h = get_counts_n_info($e, $side, $MapQ, $file, $dist_To_Soft, $target_drp, $upper_limit, $lower_limit);
-
-		$counts->{$e} = $h->{count};
-		$event_mate_info->{$e} = $h->{info};
-	}
-#print Dumper($counts);
-
-	my $max = 0;
-	my $type = "UNKNOWN";
-	my $nRP = 0;
-	my $mate_info = "NA\tNA\tNA\tNA";
-	my $summary = "GT:";
-
-	#### find max count of events and set type, nRP and info to corresponding
-	#### max count event.
-	#### also create a summary string of all counts to be added to VCF file.
-	foreach my $e (sort keys %{$counts}){
-#		if ($counts->{$e} >=i $max){
-		if ($counts->{$e} > $max){		
-			$type = $e .",". $counts->{$e};
-			$nRP = $counts->{$e};
-
-			$max = $counts->{$e};
-
-			if (length($event_mate_info->{$e})) {
-				$mate_info = $event_mate_info->{$e};
-			}
-		}
-
-		$summary .= $e .",". $counts->{$e} .":";
-	}
-#	print "done with Summary\n";
-	#### remove last colon ":" from
-	$summary =~ s/:$//;
- if (($minRP > $max)&&(!$disable_RP_only )){if ($verbose){print "FAILED BECAUSE ($minRP > $max)&&(!$disable_RP_only )"};return};
-
-	#### Run Levenstein distance on softclip in target region to find out if its a small deletion/insetion
-	#### passing 1: clip_seq, 2: chr, 3: start, 4: end, 5: ref file.
-	my $levD = new LevD;
-########################################################
-########################################################
-########################################################
-
-	#### redefine start and end location for LevD calc.
-#	$start = $plus_Reads[1]-$dist_To_Soft;
-#	$end = $plus_Reads[2];
-	my $num_bases_to_loc=0;
-	my $new_start=0;
-	my $new_end=0;
-	my $del_seq="";
-        my $start = $start_local;
-        my $end = $end_local;
-	if ($lSC=~/NA/){$lSC=0}
-
-	if ($side =~ /right/) {
-	        $levD->search($plus_Reads[3], $plus_Reads[0], $start, $end, $INPUT_FASTA);
-		$local_levD = sprintf("%.2f", $levD->{relative_edit_dist});
-	        $num_bases_to_loc=$levD->{index};
-		$new_start = $plus_Reads[2];
-                if ($plus_Reads[2]=~/^[0-9]/){$new_end=$plus_Reads[2]+$lSC};
-	}
-	else{
-		$levD->search($plus_Reads[3], $plus_Reads[0], $start, $end, $INPUT_FASTA);
-		$local_levD = sprintf("%.2f", $levD->{relative_edit_dist});
-		$num_bases_to_loc=$levD->{index};
-		if ($plus_Reads[2]=~/^[0-9]/){$new_start=$plus_Reads[2]-$lSC};
-                $new_end = $plus_Reads[2];
-	}
-	if((!$new_start)||(!$new_end)||($new_start<0)){print "FAILED AT ((!$new_start)||(!$new_end)||($new_start<0))\n";return};
-	
-	$del_seq=getBases($plus_Reads[0], $new_start,$new_end,$INPUT_FASTA);
-##############################################################################
-#	#If there is a match, where is the start position of the match?
-#
-##############################################################################
-
-
-	#if $plus_Reads[3] eq "NA", then it was found without soft-clipped reads
-	if($plus_Reads[3] !~  /NA/){
-			if (($local_levD < $levD_local_threshold)) {
-				return if (!$sv_only);
-				#### add value to summary to be written to vcf file.
-				$summary = "GT:sDel," . $plus_Reads[6];
-				$type = "sDEL";
-				###########################################################################
-				##### Printing output
-
-				#########################################
-				##### Get DNA info
-				#########################################
-				#$REF1_base = getSeq($plus_Reads[0], $plus_Reads[1], $INPUT_FASTA);
-				$REF1_base = substr($del_seq, 0, 1);
-
-				#### this is alt ref. for softclip its $plus_Reads[3]
-				$REF2_base = $del_seq;
-				$QUAL = 1/($local_levD + 0.001);
-				$QUAL = sprintf("%.2f",$QUAL);
-				$isize = length($del_seq);
-
-				#### svtype = none for sDEL
-				#### isize = length($info[3]);
-				#### nRP = NA
-				#### mate_id = NA
-				#### CTX,:DEL,:....sDEL,##
-				$INFO_1=join(";", "SVTYPE=NA", "EVENT=$type", "ISIZE=$isize");
-
-				#Add Sample infomration
-				my $FORMAT="GT:sDEL";
-				$FORMAT .= ":lSC:nSC:uRP:levD_local";
-				my $SAMPLE= "0/1:";
-				$SAMPLE .= "$plus_Reads[6]:$lSC:$nSC:$num_unmapped_pairs:$local_levD";
-
-				#### remove any white spaces.
-				$INFO_1=~s/\s//g;
-				$INFO_2=~s/\s//g;
-
-				$BND1_name =~ s/^BND/LEVD/;
-				# If left, then the start position is plus_Reads[1]-isize
-				my $start_pos=0;
-				#Make sure Ref1 and Ref2 bases are different
-				if($REF2_base eq $REF1_base){$REF1_base="NA"}
-				if($side=~/left/){$start_pos=$plus_Reads[1]-$isize}else{$start_pos=$plus_Reads[1]};		
-				print OUT join ("\t", $plus_Reads[0], $start_pos, $BND1_name, $REF2_base, $REF1_base, $QUAL, "PASS", $INFO_1,$FORMAT,$SAMPLE, "\n");
-				if ($verbose){print "No Softclipped reads here!\n"}
-				return;
-			}
-		}
-
-		#### Otherwise, look for DRP mate info
-	#if($nRP=~/NA/){print "MATE_INFO=$mate_info\tSide=$side\tline=$line\n";}
-		my @mate_info_arr = split(/\t/, $mate_info);
-		$nRP = $mate_info_arr[3];
-		my $mate_chr=$mate_info_arr[0];
-
-			if((! defined $nRP) || ($nRP =~ /na/i) || ($mate_chr =~ /NA/) ){
-			#PRINT UNKNOWN
-	if ($nRP =~ /na/i){print "Can't find SC reads\n" if ($verbose);return};
-	if ($verbose){print "There is an unknown\nNRP=$nRP Mate_CHR=$mate_chr minRP=$minRP\n"}
-				$summary .= ":unknown," . $plus_Reads[6];
-				$type = "unknown";
-				$REF1_base = getSeq($plus_Reads[0], $plus_Reads[1], $INPUT_FASTA);
-				$REF2_base = $plus_Reads[3];
-				$BND1_name =~ s/^BND/UNKNOWN/;
-				$QUAL = 1/($local_levD + 0.001);
-				$QUAL = sprintf("%.2f",$QUAL);
-				$INFO_1=join(";", "SVTYPE=unknown", "EVENT=unknown", "ISIZE=unknown");
-				#Add Sample infomration
-				my $FORMAT="GT:sDEL";
-				$FORMAT .= ":lSC:nSC:uRP:levD_local";
-				my $SAMPLE = "0/1:";
-				$SAMPLE .= "$plus_Reads[6]:$lSC:$nSC:$num_unmapped_pairs:$local_levD";
-				$SAMPLE=~s/NA/0/g;
-				#### remove any white spaces.
-				$INFO_1=~s/\s//g;
-			       #print join ("\t", $plus_Reads[0], $plus_Reads[1],  $REF2_base, $REF1_base, $QUAL, "PASS", $INFO_1,$FORMAT,$SAMPLE, "\n");
-
-				print OUT join ("\t", $plus_Reads[0], $plus_Reads[1], $BND1_name, $REF1_base, $REF2_base, $QUAL, "PASS", $INFO_1,$FORMAT,$SAMPLE, "\n");
-				return;
-
-		}
-		#### end if there is no mate info or nRP+uRP<minRP
-		if (($nRP<$minRP)&&($unmated_pairs > ($num_unmapped_pairs+$nRP))){
-			print "Something failed here\nif (($nRP<$minRP)&&($unmated_pairs > ($num_unmapped_pairs+$nRP)))\n";
-		return}
-
-		##################################################################################
-		# Find out if mates have nearby soft-clips (to refine the breakpoints)
-		##################################################################################
-		#Look for evidence of soft-clipping near mate
-		my @mate_soft_arr = ();
-		my $mate_start = 0;
-		my $mate_soft = "";
-
-		@mate_info_arr = split(/\t/, $mate_info);
-
-		#### mate start and end locations.
-		my $filename = $right_sc;
-
-		$start = $mate_info_arr[1] - $dist_To_Soft;
-		$end = $mate_info_arr[1];
-
-		if ($side =~ /right/) {
-			$start = $mate_info_arr[2];
-			$end = $mate_info_arr[2] + $dist_To_Soft;
-
-			$filename = $left_sc;
-		}
-
-		#### add levenstein distance to Summary
-	#print "Calc distal Levd\n";
-		$levD->search(rev_comp($plus_Reads[3]), $mate_info_arr[0], $start, $end, $INPUT_FASTA);
-		$distl_levD = sprintf("%.2f", $levD->{relative_edit_dist});
-	$distl_levD = "NA" if($plus_Reads[3] =~ /NA/);
-	#If there is no softclips to string match, then give 0 as quality value
-       if ($plus_Reads[3] !~ /NA/){
-			$QUAL=1/($distl_levD + 0.001);
-		}
-		else	{
-			$QUAL=0;
-		};
-	$QUAL=sprintf("%.2f",$QUAL);
-	#### looking for softclips to refine break point
-	#### if left look in right and vice-versa.
-	$cmd = qq{echo -e "$mate_info_arr[0]\t$start\t$end"};
-	$cmd .= qq{ | awk -F'\t' 'NF==3' | intersectBed -a stdin -b $filename | head -1};
-print "$cmd\n" if $verbose;
-	$mate_soft = `$cmd`;
-
-	$mate_soft =~ s/\n//g;
-	@mate_soft_arr = split(/\s/, $mate_soft);
-my $NO_MATE_SC="";
-	if(@mate_soft_arr){
-		$mate_chr = $mate_soft_arr[0];
-		$mate_start = $mate_soft_arr[1];
-                $NO_MATE_SC="APPROXIMATE";
-
-	} else{
-		@mate_info_arr = split(/\s/,$mate_info);
-		$mate_chr = $mate_info_arr[0];
-		$mate_start = $mate_info_arr[1];
-	}
-
-	#end if there is no mate info
-	return if ($mate_chr eq "");
-	#end if there is no mate info and !disable_RP_only
-	return if (($lSC =~/NA/)&&(!$disable_RP_only));
-	
-	
-	###########################################################################
-	##### Printing output
-
-	#########################################
-	# Get DNA info
-	#########################################
-	#print "PLUS_READS=$plus_Reads[0],$plus_Reads[1]\nMATE=$mate_chr,$mate_start,$INPUT_FASTA\n";
-	$REF1_base = getSeq($plus_Reads[0], $plus_Reads[1], $INPUT_FASTA);
-
-	### this is alt ref. for softclip its $plus_Reads[3]
-	$REF2_base = getSeq($mate_chr, $mate_start, $INPUT_FASTA);
-
-	#########################################
-	# print in VCF format
-	#########################################
-
-	#### abs value to account for left and right reads.
-	$isize = abs($plus_Reads[1]-$mate_start);
-	
-	my $event_type=$type;
-	$event_type=~ s/,|[0-9]//g;
-	$INFO_1=join(";", "$sv_type", "EVENT=$event_type","END=$mate_start", "ISIZE=$isize","MATEID=$BND2_name");
-	$INFO_2=join(";", "$sv_type", "EVENT=$event_type","END=$plus_Reads[1]", "ISIZE=$isize","MATEID=$BND1_name");
-
-	#### remove any white spaces.
-	#### ask: did you mean to remove space from ends? eg. trim()
-	$INFO_1=~s/\s//g;
-	$INFO_2=~s/\s//g;
-
-	$FORMAT=$summary;
- 	$FORMAT=~ s/,|[0-9]//g;
-        $FORMAT .= ":lSC:nSC:uRP:distl_levD";
-	if($NO_MATE_SC){$INFO_2 .= ":NO_MATE_SC"}
-	my $SAMPLE="0/1:";	
-	$SAMPLE .=$summary;
-#        if($NO_MATE_SC){$SAMPLE.= ":$NO_MATE_SC"}
-
-	$SAMPLE=~s/[A-Z|,|_]//g;
-        my $MATE_SAMPLE=$SAMPLE;
-        $SAMPLE .= ":$lSC:$nSC:$num_unmapped_pairs:$distl_levD";
-	$MATE_SAMPLE .=":NA:NA:NA:NA";
-	$SAMPLE=~s/::/:/g;
-	$MATE_SAMPLE=~s/::/:/g;
-	$MATE_SAMPLE=~s/NA/0/g;
-	$SAMPLE=~s/NA/0/g;
- 
-	if($type !~ /INV/){
-		$ALT_1 = join("","]",$mate_chr,":",$mate_start,"]",$REF1_base);
-		$ALT_2 = join("",$REF2_base,"[",$plus_Reads[0],":",$plus_Reads[1],"[");
-		#		2      321682 bnd_V  T   ]13:123456]T  6    PASS SVTYPE=BND
-		#		13     123456 bnd_U  C   C[2:321682[   6    PASS SVTYPE=BND
-	} else {
-		$ALT_1 = join("", "]", $plus_Reads[0], ":", $plus_Reads[1], "]", $REF2_base);
-		$ALT_2 = join("", $REF1_base, "[", $mate_chr, ":", $mate_start, "[");
-	}
-
-	if(($mate_chr) && ($plus_Reads[0])){
-		print OUT join ("\t", $plus_Reads[0], $plus_Reads[1], $BND1_name, $REF1_base, $ALT_1, $QUAL,"PASS", $INFO_1, $FORMAT,$SAMPLE,"\n");
-		print OUT join ("\t", $mate_chr, $mate_start, $BND2_name, $REF2_base, $ALT_2, $QUAL, "PASS", $INFO_2, $FORMAT,$MATE_SAMPLE,"\n");
-	}
-}
-
-###############################################################################
-###############################################################################
-sub get_counts_n_info {
-        my ($event, $side, $mapQ, $file, $dist, $target, $upL, $lwL) = @_;
-
-        my $mate_info = "";
-        my $cmd = "";
-
-        if ($event =~ /^CTX$/i) {
-                #print "CTX side $side\n";
-                if ($side =~ /right/i) {
-                        $cmd = qq{samtools view $new_blacklist -q $mapQ -f 32 -F 1536 $file $target};
-                        $cmd .= qq{ | perl -ane 'if(\$F[6] ne "="){\$end=\$F[7]+1; print join ("\\t",\$F[6],\$F[7],\$end,"\\n")}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info=`$cmd`;
-                } else {
-                        $cmd = qq{ samtools view $new_blacklist -q $mapQ -f 16 -F 1536 $file $target};
-                        $cmd .= qq{ | perl -ane 'if(\$F[6] ne "="){\$end=\$F[7]+1; print join ("\\t",\$F[6],\$F[7],\$end,"\\n")}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info=`$cmd`;
-                }
-        } elsif ($event =~ /^DEL$/i) {
-                #print "DEL side $side\n";
-                if ($side =~ /right/i) {
-                        $cmd = qq{samtools view $new_blacklist -q $mapQ  -f 32 -F 1552 $file $target};
-                        $cmd .= qq{ | awk '{OFS="\\t"}{if((\$7 ~ /=/)&&(\$9>$upL)){end=\$8+1;print \$3,\$8,end}}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info=`$cmd`;
-                } else {
-                        $cmd = qq{samtools view $new_blacklist -q $mapQ -F 1568 -f 16 $file $target};
-                        $cmd .= qq{ | awk '{OFS="\\t"} {if((\$7 ~ /=/)&&(\$9<-$upL)){end=\$8+1;print \$3,\$8,end}}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist_To_Soft -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-
-                        $mate_info=`$cmd`;
-                }
-        } elsif ($event =~ /^INS$/i) {
-                #print "INS side $side\n";
-                if ($side =~ /right/i) {
-                        $cmd = qq{samtools view $new_blacklist -q $mapQ -f 32 -F 1552 $file $target};
-                        $cmd .= qq{ | awk '{OFS="\\t"}{if((\$7 ~ /=/)&&(\$9<$lwL && \$9 > 0 )){end=\$8+1;print \$3,\$8,end}}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info = `$cmd`;
-                } else {
-                        $cmd = qq {samtools view $new_blacklist -q $mapQ -f 16 -F 1568 $file $target};
-                        $cmd .= qq{ | awk '{OFS="\\t"}{if((\$7 ~ /=/)&&(\$9>-$lwL && \$9 < 0 )){end=\$8+1;print \$3,\$8,end}}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info = `$cmd`;
-                }
-        } elsif ($event =~ /^INV$/i) {
-                #print "INV side $side\n";
-                if ($side =~ /right/i) {
-                        $cmd = qq{samtools view $new_blacklist -q $mapQ  -F 1596 $file $target};
-                        $cmd .= qq{ | awk '{OFS="\\t"}{if((\$7 ~ /=/)){end=\$8+1;print \$3,\$8,end}}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist_To_Soft -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info = `$cmd`;
-                } else {
-                        $cmd = qq{samtools view $new_blacklist -q $mapQ -f 48 -F 1548 $file $target};
-                        $cmd .= qq{ | awk '{OFS="\\t"}{if((\$7 ~ /=/)){end=\$8+1;print \$3,\$8,end}}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info = `$cmd`;
-                }
-        } elsif ($event =~ /^TDUP$/i) {
-                #print "TDUP side $side\n";
-                if ($side =~ /right/i) {
-                        $cmd = qq{samtools view $new_blacklist -q $mapQ -f 32 -F 1552 $file $target};
-#			$cmd .= qq{ | awk '{OFS="\\t"}{if((\$7 ~ /=/)&&(\$9>$upL)){end=\$8+1;print \$3,\$8,end}}'};
-			$cmd .= qq{ | awk '{OFS="\\t"}{if((\$7 ~ /=/)&&(\$4>\$8)&&(\$9<0)&& (\$9>$upL)){end=\$8+1;print \$3,\$8,end}}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info = `$cmd`;
-                } else {
-                        $cmd = qq{samtools view $new_blacklist -q $mapQ -f 16 -F 1568 $file $target};
-#                        $cmd .= qq{ | awk '{OFS="\\t"}{if((\$7 ~ /=/)&&(\$9<-$upL )){end=\$8+1;print \$3,\$8,end}}'};
-                        $cmd .= qq{ | awk '{OFS="\\t"}{if((\$7 ~ /=/)&&(\$4<\$8)&&(\$9>0)&&(\$9>$upL)){end=\$8+1;print \$3,\$8,end}}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info = `$cmd`;
-                }
-        } elsif ($event =~ /^NOV_INS$/i) {
-                #print "NOV_INS side $side\n";
-                if ($side =~ /right/i) {
-                        $cmd = qq{samtools view $new_blacklist -q $mapQ -f 8 -F 1552 $file $target};
-                        $cmd .= qq{ | awk '{OFS="\\t"}{end=\$8+1;print \$3,\$8,end}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info = `$cmd`;
-                } else {
-                        $cmd = qq{samtools view $new_blacklist -q $mapQ  -f 24 -F 1536 $file $target};
-                        $cmd .= qq{ | awk '{OFS="\\t"}{end=\$8+1;print \$3,\$8,end}'};
-                        $cmd .= qq{ | sortBed | mergeBed -d $dist -n | sort -k4nr | head -1};
-#if($verbose){print "$cmd\n"}
-                        $mate_info = `$cmd`;
-                }
-        }
-
-        $mate_info=~s/\n//g;
-        my @tmp=split(/\t/, $mate_info);
-
-        my $counts = 0;
-
-        if (defined $tmp[3]) {
-                $tmp[3] =~ s/\n//g;
-
-                $counts = $tmp[3] if (length($tmp[3]));
-        }
-        return ({count=>$counts, info=>$mate_info});                                                                                                                                
-}
diff -r ddc587fed387 -r ac5bcbd14b51 softsearch.xml
--- a/softsearch.xml	Thu May 29 06:50:23 2014 -0400
+++ /dev/null	Thu Jan 01 00:00:00 1970 +0000
@@ -1,53 +0,0 @@
-<?xml version="1.0"?>
-<tool id="SoftSearch" name="SoftSearch">
-  <description>for structure variation</description>
-  <command> #if $source.index_source=="history" 
-	        samtools index $bam_file ; samtools faidx $source.history_fasta_file ; $inc | ~/2.4/src/SoftSearch.pl -l $min_length_soft_clip -q $min_map_quality -r $min_depth_soft_clip_loc -m $min_no_discordant_read -s $no_sd_consider_discordant -b $bam_file -f $source.history_fasta_file -o $out_file1
-	    #else
-		samtools index $bam_file ; samtools faidx $source.ref_fasta.fields.path ; $inc | ~/2.4/src/SoftSearch.pl -l $min_length_soft_clip -q $min_map_quality -r $min_depth_soft_clip_loc -m $min_no_discordant_read -s $no_sd_consider_discordant -b $bam_file -f $source.ref_fasta.fields.path -o $out_file1
-	    #end if		
- </command>
-  <inputs>
-      <param name="bam_file" type="data"   format="bam" label="BAM Files" />
-	 <conditional name="source">
-            <param name="index_source" type="select" label="Choose the source for the reference list">
-                <option value="cached">Locally cached</option>
-                <option value="history">History</option>
-            </param>
-            <when value="history">
-                <param format="fasta" name="history_fasta_file" type="data" label="Fasta file from history."/>
-            </when>
-            <when value="cached">
-                  <param name="ref_fasta" type="select" >
-                    <options from_data_table="fasta_indexes">
-                    <validator type="no_options" message="No Fasta file is available" />
-                    </options>
-                  </param>
-            </when>
-        </conditional>
-      <param name="inc" type="hidden" value="n=$RANDOM" />
-     	<param name="min_length_soft_clip" type="integer" value="10" label="-l Minimum length of soft-clipped segment [5]" /> 
-        <param name="min_map_quality" type="integer" value="20" label="-q Minimum mapping quality [20]" /> 	
-	<param name="min_depth_soft_clip_loc" type="integer" value="10" label="-r Minimum depth of soft-clipped reads at position [5]" /> 
-	<param name="min_no_discordant_read" type="integer" value="10" label="-m Minimum number of discordant read pairs [5]" /> 
-	<param name="no_sd_consider_discordant" type="integer" value="4" label="-s Number of sd away from mean to be considered discordant" />       
-  </inputs>
-  <outputs>
-      <data format="vcf" name="out_file1" />
-  </outputs>
-  <help>
-  </help>
-</tool>
-
-
-
-
-
-
-
-
-
-
-
-
-