Mercurial > repos > proteore > proteore_goprofiles
annotate goprofiles.xml @ 10:2138e0035e57 draft
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author | proteore |
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date | Mon, 04 Mar 2019 08:20:52 -0500 |
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1 <tool id="goProfiles" name="Statistical analysis of functional profiles" version="2019.02.27.1"> |
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2 <description>(Human, Mouse) [goProfiles]</description> |
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3 <requirements> |
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4 <requirement type="package" version="3.4.1">R</requirement> |
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5 <requirement type="package" version="3.5.0">bioconductor-org.hs.eg.db</requirement> |
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6 <requirement type="package" version="3.5.0">bioconductor-org.mm.eg.db</requirement> |
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7 <!--requirement type="package" version="3.5.0">bioconductor-org.rn.eg.db</requirement--> |
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8 <requirement type="package" version="1.40.0">bioconductor-annotationdbi</requirement> |
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9 <requirement type="package" version="2.38.0">bioconductor-biobase</requirement> |
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10 <requirement type="package" version="1.38.0">goprofiles</requirement> |
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11 |
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12 </requirements> |
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13 <stdio> |
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14 <exit_code range="1:" /> |
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15 </stdio> |
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16 <command><![CDATA[ |
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17 Rscript $__tool_directory__/goprofiles.R |
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18 #if $input.ids == "text" |
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19 --input_type="text" |
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20 --input="$input.text" |
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21 #else |
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22 --input_type="file" |
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23 --input="$input.file" |
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24 --ncol="$input.ncol" |
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25 --header="$input.header" |
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26 #end if |
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27 |
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28 --id_type="$input.id_type" |
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29 --onto_opt="$onto_opt" |
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30 --plot_opt="$plot_opt" |
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31 --level="$level" |
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32 --per="$per" |
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33 --title="$title" |
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34 --duplicate="$duplicate" |
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35 --text_output="$text_output" |
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36 --species="$species" > $log |
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37 |
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38 ]]></command> |
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39 <inputs> |
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40 <conditional name="input" > |
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41 <param name="ids" type="select" label="Enter your IDs (Entrez Gene ID or UniProt Accession number)" help="Copy/paste or from a file (e.g. table)" > |
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42 <option value="text">Copy/paste your IDs</option> |
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43 <option value="file" selected="true">Input file containing your IDs</option> |
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44 </param> |
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45 <when value="text" > |
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46 <param name="text" type="text" label="Copy/paste your IDs" help='IDs must be separated by spaces into the form field, for example: P31946 P62258' > |
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47 <sanitizer> |
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48 <valid initial="string.printable"> |
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49 <remove value="'"/> |
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50 </valid> |
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51 <mapping initial="none"> |
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52 <add source="'" target="__sq__"/> |
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53 </mapping> |
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54 </sanitizer> |
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55 </param> |
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56 <param name="id_type" type="select" label="Select type of IDs list" > |
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57 <option value="Entrez">Entrez Gene ID</option> |
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58 <option value="UniProt">UniProt Accession number</option> |
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59 </param> |
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60 </when> |
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61 <when value="file" > |
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62 <param name="file" type="data" format="txt,tabular" label="Select your file" help="" /> |
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63 <param name="ncol" type="text" value="c1" label="Column number of IDs" help='For example, fill in "c1" if it is the first column, "c2" if it is the second column and so on'> |
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64 <validator type="regex" message="Please enter a column number, for example: 'c1' for the first column">[c]{0,1}[0-9]+</validator> |
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65 </param> |
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66 <param name="header" type="boolean" checked="true" truevalue="true" falsevalue="false" label="Does file contain header?" /> |
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67 <param name="id_type" type="select" label="Select type of IDs list" > |
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68 <option value="Entrez">Entrez Gene ID</option> |
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69 <option value="UniProt">Uniprot Accession number</option> |
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70 </param> |
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71 </when> |
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72 </conditional> |
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73 <param name="duplicate" type="boolean" label="Remove duplicated IDs" truevalue="TRUE" falsevalue="FALSE" /> |
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74 <param name="species" type="select" label="Species"> |
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75 <option value="org.Hs.eg.db">Human (Homo sapiens)</option> |
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76 <option value="org.Mm.eg.db">Mouse (Mus musculus)</option> |
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77 <!--option value="org.Rn.eg.db">Rat (Rattus norvegicus)</option--> |
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78 </param> |
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79 <param type="select" name="onto_opt" label="Select GO terms category" multiple="True" display="checkboxes" optional="false" > |
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80 <option value="CC">Cellular Component (CC)</option> |
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81 <option value="MF">Molecular Function (MF)</option> |
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82 <option value="BP">Biological Process (BP)</option> |
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83 </param> |
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84 <param type="select" name="level" label="Ontology level (the higher this number, the deeper the GO level)" > |
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85 <option value="1">1</option> |
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86 <option value="2" selected="True">2</option> |
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87 <option value="3">3</option> |
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88 <option value="4">4</option> |
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89 <option value="5">5</option> |
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90 <option value="6">6</option> |
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91 </param> |
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92 <param type="boolean" name="per" label="Plot absolute frequencies?" truevalue="TRUE" falsevalue="FALSE" /> |
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93 <param type="text" name="title" label="Enter title of your figure"> |
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94 <sanitizer invalid_char=''> |
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95 <valid initial="string.printable"> |
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96 <remove value="'"/> |
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97 <remove value="/"/> |
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98 </valid> |
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99 <mapping> |
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100 <add source=" " target=""/> |
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101 </mapping> |
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102 </sanitizer> |
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103 </param> |
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104 <param type="select" name="plot_opt" label="Plot file format"> |
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105 <option value="PNG" selected="True">png</option> |
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106 <option value="JPEG">jpeg</option> |
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107 <option value="PDF">pdf</option> |
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108 </param> |
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109 |
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110 </inputs> |
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111 <outputs> |
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112 <data name="log" format="tsv" label="goProfiles log" /> |
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113 <collection type="list" label="goProfiles text files" name="text_output"> |
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114 <discover_datasets pattern="(?P<designation>.+\.tsv)" ext="tsv"/> |
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115 </collection> |
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116 <collection type="list" label="goProfiles diagram output" name="output" > |
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117 <discover_datasets pattern="(?P<designation>.+\.png)" ext="png" /> |
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118 <discover_datasets pattern="(?P<designation>.+\.jpeg)" ext="jpg" /> |
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119 <discover_datasets pattern="(?P<designation>.+\.pdf)" ext="pdf" /> |
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120 </collection> |
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121 </outputs> |
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122 <tests> |
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123 <test> |
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124 <conditional name="input"> |
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125 <param name="ids" value="file" /> |
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126 <param name="file" value="ID_Converted_FKW_Lacombe_et_al_2017_OK.txt" /> |
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127 <param name="ncol" value="c1" /> |
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128 <param name="header" value="true" /> |
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129 <param name="id_type" value="UniProt" /> |
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130 </conditional> |
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131 <param name="duplicate" value="false"/> |
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132 <param name="onto_opt" value="CC,MF,BP" /> |
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133 <param name="level" value="2" /> |
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134 <param name="per" value="true" /> |
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135 <param name="title" value="Test" /> |
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136 <param name="plot_opt" value="PDF" /> |
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137 <output_collection name="output" type="list" > |
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138 <element name="profile.BP.pdf" file="profile.BP.pdf" ftype="pdf" compare="sim_size"/> |
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139 <element name="profile.CC.pdf" file="profile.CC.pdf" ftype="pdf" compare="sim_size"/> |
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140 <element name="profile.MF.pdf" file="profile.MF.pdf" ftype="pdf" compare="sim_size"/> |
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141 </output_collection> |
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142 <output name="log" file="GO_Profile_text_output.tsv"/> |
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143 </test> |
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144 </tests> |
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145 <help><![CDATA[ |
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146 |
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147 **Description** |
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148 |
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149 This tool is based on the goProfiles R package; it performs statistical analysis of functional profiles based on Gene Ontology (GO). Functional profile at a given GO level is obtained by counting the |
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150 number of identifiers having a hit in each category of this level. |
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151 |
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152 ----- |
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153 |
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154 **Input** |
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155 |
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156 Two modes are allowed: either by copy/pasting your IDs (separated by a space) or by supplying a tabular file (.csv, .tsv, .txt, .tab) including your IDs (identifiers). |
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157 Only entrez gene ID (e.g : 4151) or Uniprot accession number (e.g. P31946) are allowed. If your list is not in this form, please use the ID_Converter tool of ProteoRE. |
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158 |
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159 ----- |
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160 |
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161 **Parameters** |
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162 |
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163 "Species": enter the sepcies you are working on; Homo sapiens and Mus musculus supported (Rattus norvegicus coming soon) |
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164 |
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165 "Select GO terms category": you can choose one or more GO categories which are Biological Process (BP), Cellular Component (CC) and Molecular Function (MF) |
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166 |
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167 "Ontology level (the higher this number, the deeper the GO level)": correspond to the level of GO hierarchy (from 1 to 6). In general the higher the level, the more semantically specific the term is. |
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168 |
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169 ----- |
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170 |
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171 **Ouput** |
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172 |
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173 Diagram output: graphical output in the form of bar-plot or dot-plot (png (default format), jpeg or pdf format), one figure for each GO category. |
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174 |
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175 text output: with the following information GO category description (e.g.BP.Description), GO term identifier (e.g. BP.GOID) and GO term frequency (e.g. BP.Frequency) |
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176 |
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177 ----- |
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178 |
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179 .. class:: infomark |
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180 |
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181 **Authors** |
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182 |
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183 Salicrú M, Ocaña J, Sánchez-Pla A. Comparison of lists of genes based on functional profiles. BMC Bioinformatics. 2011. 12:401. doi:10.1186/1471-2105-12-401. PubMed PMID: 21999355 |
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184 |
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185 ----- |
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186 |
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187 .. class:: infomark |
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188 |
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189 **Galaxy integration** |
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190 |
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191 T.P. Lien Nguyen, Florence Combes, Yves Vandenbrouck CEA, INSERM, CNRS, Grenoble-Alpes University, BIG Institute, FR |
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192 |
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193 Sandra Dérozier, Olivier Rué, Christophe Caron, Valentin Loux INRA, Paris-Saclay University, MAIAGE Unit,Migale Bioinformatics platform, FR |
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194 |
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195 This work has been partially funded through the French National Agency for Research (ANR) IFB project. |
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196 |
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197 Contact support@proteore.org for any questions or concerns about the Galaxy implementation of this tool. |
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198 |
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199 ]]></help> |
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200 <citations> |
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201 </citations> |
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202 </tool> |