# HG changeset patch # User q2d2 # Date 1673649754 0 # Node ID 2572fc0f88f2ca490518a314053b5485bf8aa913 # Parent 0cba463cfb726d2cac994593bd386c645597e3aa planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__deblur commit 69da7976573cc07a363ac66bdacc9269d7cd3732 diff -r 0cba463cfb72 -r 2572fc0f88f2 qiime2__deblur__denoise_16S.xml --- a/qiime2__deblur__denoise_16S.xml Mon Aug 29 19:25:20 2022 +0000 +++ b/qiime2__deblur__denoise_16S.xml Fri Jan 13 22:42:34 2023 +0000 @@ -1,31 +1,31 @@ - + Deblur sequences using a 16S positive filter. - quay.io/qiime2/core:2022.8 + quay.io/qiime2/core:2022.11 q2galaxy version deblur - q2galaxy run deblur denoise_16S '$inputs' + q2galaxy run deblur denoise_16S '$inputs' + - hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['SampleData[JoinedSequencesWithQuality]', 'SampleData[PairedEndSequencesWithQuality]', 'SampleData[SequencesWithQuality]'] @@ -47,7 +47,34 @@ - + + + + + + + + + + + + + + + + + + + + + + + + + + + + QIIME 2: deblur denoise-16S =========================== @@ -66,6 +93,36 @@ ------------ Perform sequence quality control for Illumina data using the Deblur workflow with a 16S reference as a positive filter. Only forward reads are supported at this time. The specific reference used is the 88% OTUs from Greengenes 13_8. This mode of operation should only be used when data were generated from a 16S amplicon protocol on an Illumina platform. The reference is only used to assess whether each sequence is likely to be 16S by a local alignment using SortMeRNA with a permissive e-value; the reference is not used to characterize the sequences. +Examples: +--------- + +denoise_16S +*********** +Using the ``qiime2 deblur denoise-16S`` tool: + #. Set *"demultiplexed_seqs"* to ``#: demux-filtered.qza`` + #. Set *"trim_length"* to ``120`` + #. Expand the ``additional options`` section + + - Set *"sample_stats"* to ``Yes`` + + #. Press the ``Execute`` button. + +Once completed, for each new entry in your history, use the ``Edit`` button to set the name as follows: + (Renaming is optional, but it will make any subsequent steps easier to complete.) + + .. list-table:: + :align: left + :header-rows: 1 + + * - History Name + - *"Name"* to set (be sure to press ``Save``) + * - ``#: qiime2 deblur denoise-16S [...] : table.qza`` + - ``table.qza`` + * - ``#: qiime2 deblur denoise-16S [...] : representative_sequences.qza`` + - ``representative-sequences.qza`` + * - ``#: qiime2 deblur denoise-16S [...] : stats.qza`` + - ``denoising-stats.qza`` + | diff -r 0cba463cfb72 -r 2572fc0f88f2 test-data/.gitkeep diff -r 0cba463cfb72 -r 2572fc0f88f2 test-data/denoise_16S.test0.demux-filtered.qza Binary file test-data/denoise_16S.test0.demux-filtered.qza has changed diff -r 0cba463cfb72 -r 2572fc0f88f2 test-data/visualize_stats.test0.deblur-stats.qza Binary file test-data/visualize_stats.test0.deblur-stats.qza has changed