# HG changeset patch # User q2d2 # Date 1661803351 0 # Node ID df674248c5211bf48c367a8e06d374686335e561 planemo upload for repository https://github.com/qiime2/galaxy-tools/tree/main/tools/suite_qiime2__fragment_insertion commit 9023cfd83495a517fbcbb6f91d5b01a6f1afcda1 diff -r 000000000000 -r df674248c521 qiime2__fragment_insertion__filter_features.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/qiime2__fragment_insertion__filter_features.xml Mon Aug 29 20:02:31 2022 +0000 @@ -0,0 +1,80 @@ + + + + + Filter fragments in tree from table. + + quay.io/qiime2/core:2022.8 + + q2galaxy version fragment_insertion + q2galaxy run fragment_insertion filter_features '$inputs' + + + + + + + + + hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['FeatureTable[Frequency]'] + + + + + + hasattr(value.metadata, "semantic_type") and value.metadata.semantic_type in ['Phylogeny[Rooted]'] + + + + + + + + +QIIME 2: fragment-insertion filter-features +=========================================== +Filter fragments in tree from table. + + +Outputs: +-------- +:filtered_table.qza: The input table minus those fragments that were not part of the tree. This feature-table can be used for downstream analyses like phylogenetic alpha- or beta- diversity computation. +:removed_table.qza: Those fragments that got removed from the input table, because they were not part of the tree. This table is mainly used for quality control, e.g. to inspect the ratio of removed reads per sample from the input table. You can ignore this table for downstream analyses. + +| + +Description: +------------ +Filters fragments not inserted into a phylogenetic tree from a feature-table. Some fragments computed by e.g. Deblur or DADA2 are too remote to get inserted by SEPP into a reference phylogeny. To be able to use the feature-table for downstream analyses like computing Faith's PD or UniFrac, the feature-table must be cleared of fragments that are not part of the phylogenetic tree, because their path length can otherwise not be determined. Typically, the number of rejected fragments is low (<= 10), but it might be worth to inspect the ratio of reads assigned to those rejected fragments. + + +| + + + + 10.1128/mSystems.00021-18 + 10.1371/journal.pone.0031009 + 10.1371/journal.pcbi.1002195 + @article{cite4, + author = {Matsen, Frederick A and Kodner, Robin B and Armbrust, E Virginia}, + journal = {BMC bioinformatics}, + number = {1}, + pages = {538}, + publisher = {BioMed Central}, + title = {pplacer: linear time maximum-likelihood and Bayesian phylogenetic placement of sequences onto a fixed reference tree}, + volume = {11}, + year = {2010} +} + + 10.1038/s41587-019-0209-9 + + diff -r 000000000000 -r df674248c521 test-data/.gitkeep