annotate phyloseq_2_deseq2.xml @ 1:a55b89bc9788 draft

"planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 9767e7d8ab085c7d6323b8323d9047cb68bc0211"
author qfabrepo
date Wed, 16 Sep 2020 06:34:40 +0000
parents 1f0569e94be4
children 7e24242ffa65
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1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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1 <tool id="phyloseq_DESeq2" name="DESeq2" version="1.22.3" hidden="false">
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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2 <description>Differential Analysis</description>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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3 <requirements>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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4 <requirement type="package" version="3.4.1">r-base</requirement>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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5 <requirement type="package" version="1.22.3">bioconductor-phyloseq</requirement>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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6 <requirement type="package" version="1.18.1">bioconductor-deseq2</requirement>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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7 <requirement type="package" version="1.20.0">r-getopt</requirement>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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8 <requirement type="package" version="9.18">ghostscript</requirement>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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9 </requirements>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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10 <version_command><![CDATA[
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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11 echo $(R --version | grep version | grep -v GNU)", phyloseq version" $(R --vanilla --slave -e "library(phyloseq); cat(sessionInfo()\$otherPkgs\$phyloseq\$Version)" 2> /dev/null | grep -v -i "WARNING: ")
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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12 ]]></version_command>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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13 <command detect_errors="exit_code"><![CDATA[
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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14 Rscript '${__tool_directory__}/phyloseq_2_deseq2.r'
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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15 --biomfile='$biom_input'
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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16 --metafile='$metadata_input'
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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17 --factor="${factor}"
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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18 --test="${test}"
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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19 --fitType="${fitType}"
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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20 --cutoff='${cutoff}'
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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21 --result='$DE_table'
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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22 --normalisedResult='$normalised_table'
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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23 ]]></command>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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24
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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25 <inputs>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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26 <param format="biom1" name="biom_input" type="data" label="BIOM file"/>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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27 <param format="tabular" name="metadata_input" type="data" label="Metadata file"/>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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28 <param name="factor" type="data_column" data_ref="metadata_input" use_header_names="TRUE" label="factor for differential analysis testing"/>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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29 <param name="test" type="select" display="radio" label="select a test statistic">
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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30 <option value="Wald" selected="true">Wald</option>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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31 <option value="LRT">LRT</option>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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32 </param>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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33 <param name="fitType" type="select" display="radio" label="select a fit Type for dispersions">
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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34 <option value="parametric" selected="true">parametric</option>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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35 <option value="local">local</option>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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36 </param>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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37 <param name="cutoff" value="0.05" type="float" label="cutoff value for differential analysis"/>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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38 </inputs>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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39
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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40 <outputs>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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41 <data format="tabular" name="DE_table" label="${tool.name} DE.txt"/>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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42 <data format="tabular" name="normalised_table" type="float" label="${tool.name} Normalised Table.txt"/>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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43 </outputs>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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44
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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45 <tests>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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46 <test> <!-- Test : Test BIOM format input -->
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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47 <param name="biom_input" value="test.biom" ftype="biom1" />
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48 <param name="metadata_input" value="metadata.txt"/>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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49 <param name="factor" value="3" />
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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50 <param name="test" value="Wald" />
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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51 <param name="fitType" value="parametric" />
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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52 <param name="cutoff" value="0.05"/>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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53 <output name="DE_table" ftype="tabular" file="DE_table.txt" />
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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54 <output name="norm_DE_table" ftype="tabular" file="norm_DE_table.txt" />
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55 </test>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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56 </tests>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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57 <help>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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58 **What it does**
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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59 Use BIOM and metadata file as input to perform a differential analysis using R package, DESeq2_ and Phyloseq_.
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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60
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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61 .. _DESeq2: https://bioconductor.org/packages/release/bioc/html/DESeq2.html
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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62 .. _Phyloseq: https://joey711.github.io/phyloseq/index.html
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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64 -----
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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66 **Input**
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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68 - **BIOM file** - select BIOM file
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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69 - **Metadata file** - metadata file contains experimental information.
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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70 - **factor for differential analysis testing** - select a factor for differential analysis testing
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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71 - **Select a test statistic** - e.g. Wald or LRT
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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72 - **Select a fit Type for dispersions** - e.g. parametric or local
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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73 - **cutoff value for differential analysis** - e.g. 0.05
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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75 -----
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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78 =========
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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79 Resources
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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80 =========
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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81
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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82 **Wrapper Authors**
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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83
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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84 QFAB Bioinformatics (support@qfab.org)
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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85 </help>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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86 <citations>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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87 <citation type="doi">10.18129/B9.bioc.phyloseq</citation>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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88 <citation type="doi">10.1186/s13059-014-0550-8</citation>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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89 </citations>
1f0569e94be4 "planemo upload for repository https://github.com/QFAB-Bioinformatics/metaDEGalaxy/tree/master/phyloseq_2_deseq2 commit 8bd68662b72404f6291e9628327dcb109b5fa55e"
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90 </tool>