Mercurial > repos > rmarenco > hubarchivecreator

annotate hubArchiveCreator.py @ 13:25809f699cb3 draft

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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
author rmarenco
date Thu, 11 Aug 2016 19:02:29 -0400
parents d05236b15f81
children 3233451a3bd6
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1 #!/usr/bin/python
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2 # -*- coding: utf8 -*-
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4 """
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5 This Galaxy tool permits to prepare your files to be ready for
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6 Assembly Hub visualization.
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7 Program test arguments:
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8 hubArchiveCreator.py -g test-data/augustusDbia3.gff3 -f test-data/dbia3.fa -d . -u ./tools -o output.html
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9 """
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11 import argparse
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12 import collections
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13 import json
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14 import logging
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15 import os
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16 import sys
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18 # Internal dependencies
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19 from Bam import Bam
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20 from BedSimpleRepeats import BedSimpleRepeats
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21 from Bed import Bed
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22 from BigWig import BigWig
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23 from util.Fasta import Fasta
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24 from util.Filters import TraceBackFormatter
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25 from Gff3 import Gff3
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26 from Gtf import Gtf
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27 from TrackHub import TrackHub
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29 # TODO: Verify each subprocessed dependency is accessible [gff3ToGenePred, genePredToBed, twoBitInfo, faToTwoBit, bedToBigBed, sort
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32 def main(argv):
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33 # Command Line parsing init
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34 parser = argparse.ArgumentParser(description='Create a foo.txt inside the given folder.')
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36 # Reference genome mandatory
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37 parser.add_argument('-f', '--fasta', help='Fasta file of the reference genome')
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39 # GFF3 Management
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40 parser.add_argument('--gff3', action='append', help='GFF3 format')
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42 # GTF Management
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43 parser.add_argument('--gtf', action='append', help='GTF format')
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45 # Bed4+12 (TrfBig)
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46 parser.add_argument('--bedSimpleRepeats', action='append', help='Bed4+12 format, using simpleRepeats.as')
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48 # Generic Bed (Blastx transformed to bed)
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49 parser.add_argument('--bed', action='append', help='Bed generic format')
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51 # BigWig Management
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52 parser.add_argument('--bigwig', action='append', help='BigWig format')
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54 # Bam Management
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55 parser.add_argument('--bam', action='append', help='Bam format')
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57 # TODO: Check if the running directory can have issues if we run the tool outside
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58 parser.add_argument('-d', '--directory',
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59 help='Running tool directory, where to find the templates. Default is running directory')
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60 parser.add_argument('-u', '--ucsc_tools_path',
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61 help='Directory where to find the executables needed to run this tool')
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62 parser.add_argument('-e', '--extra_files_path',
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63 help='Name, in galaxy, of the output folder. Where you would want to build the Track Hub Archive')
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64 parser.add_argument('-o', '--output', help='Name of the HTML summarizing the content of the Track Hub Archive')
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66 parser.add_argument('-j', '--data_json', help='Json containing the metadata of the inputs')
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67
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68 parser.add_argument('--user_email', help='Email of the user who launched the Hub Archive Creation')
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70 parser.add_argument('--genome_name', help='UCSC Genome Browser assembly ID')
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72 parser.add_argument('--debug_mode', action='store_true', help='Allow more details about the errors')
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74 # Begin init variables
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76 toolDirectory = '.'
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77 extra_files_path = '.'
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79 # Get the args passed in parameter
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80 args = parser.parse_args()
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82 extra_files_path = args.extra_files_path
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83 toolDirectory = args.directory
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85 #### Logging management ####
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86 # If we are in Debug mode, also print in stdout the debug dump
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88 configure_logger(extra_files_path=extra_files_path, debug=args.debug_mode)
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90 #### END Logging management ####
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92 array_inputs_reference_genome = json.loads(args.fasta)
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93
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94 # TODO: Replace these with the object Fasta
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95 input_fasta_file = array_inputs_reference_genome["false_path"]
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96 input_fasta_file_name = sanitize_name_input(array_inputs_reference_genome["name"])
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97 genome_name = sanitize_name_input(args.genome_name)
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98
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99 reference_genome = Fasta(input_fasta_file,
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100 input_fasta_file_name, genome_name)
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101
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102 user_email = args.user_email
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103
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104
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105 # TODO: Use a class to have a better management of the structure of these inputs
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106 # These inputs are populated in the Galaxy Wrapper xml and are in this format:
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107 # ARRAY[DICT{FILE_PATH: DICT{NAME: NAME_VALUE, EXTRA_DATA: EXTRA_DATA_VALUE}}]
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108 # EXTRA_DATA could be anything, for example the index of a BAM => {"index", FILE_PATH}
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109 array_inputs_gff3 = args.gff3
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110 array_inputs_bed_simple_repeats = args.bedSimpleRepeats
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111 array_inputs_bed_generic = args.bed
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112 array_inputs_gtf = args.gtf
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113 array_inputs_bam = args.bam
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114 array_inputs_bigwig = args.bigwig
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115
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116 outputFile = args.output
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117
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118 json_inputs_data = args.data_json
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119
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120 inputs_data = json.loads(json_inputs_data)
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121 # We remove the spaces in ["name"] of inputs_data
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122 sanitize_name_inputs(inputs_data)
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123
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124
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125 # TODO: Check here all the binaries / tools we need. Exception if missing
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126
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127 # Create the Track Hub folder
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128 trackHub = TrackHub(reference_genome, user_email, outputFile, extra_files_path, toolDirectory)
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129
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130 all_datatype_dictionary = {}
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131
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132 for (inputs, datatype_class) in [(array_inputs_gff3, Gff3),
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133 (array_inputs_bed_simple_repeats, BedSimpleRepeats),
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134 (array_inputs_bed_generic, Bed),
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135 (array_inputs_gtf, Gtf),
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136 (array_inputs_bam, Bam),
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137 (array_inputs_bigwig, BigWig)]:
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138 if inputs:
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139 all_datatype_dictionary.update(create_ordered_datatype_objects(datatype_class, inputs, inputs_data))
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140
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141 # Create Ordered Dictionary to add the tracks in the tool form order
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142 all_datatype_ordered_dictionary = collections.OrderedDict(all_datatype_dictionary)
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143
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144 for index, datatypeObject in all_datatype_ordered_dictionary.iteritems():
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145 trackHub.addTrack(datatypeObject.track.trackDb)
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146
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147 # We process all the modifications to create the zip file
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148 #trackHub.createZip()
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149
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150 # We terminate le process and so create a HTML file summarizing all the files
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151 trackHub.terminate()
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152
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153 logging.debug('#### End of HubArchiveCreator Debug Mode: Bye! ####')
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154
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155 sys.exit(0)
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156
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157
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158 def sanitize_name_input(string_to_sanitize):
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159 return string_to_sanitize \
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160 .replace("/", "_") \
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161 .replace(" ", "_")
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162
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163
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164 def sanitize_name_inputs(inputs_data):
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165 """
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166 Sometimes output from Galaxy, or even just file name from user have spaces
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167 Also, it can contain '/' character and could break the use of os.path function
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168 :param inputs_data: dict[string, dict[string, string]]
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169 :return:
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170 """
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171 for key in inputs_data:
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172 inputs_data[key]["name"] = sanitize_name_input(inputs_data[key]["name"])
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173
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174
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175 def create_ordered_datatype_objects(ExtensionClass, array_inputs, inputs_data):
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176 """
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177 Function which executes the creation all the necessary files / folders for a special Datatype, for TrackHub
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178 and update the dictionary of datatype
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179 :param ExtensionClass: T <= Datatype
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180 :param array_inputs: list[string]
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181 :param inputs_data:
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182 """
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183
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184 datatype_dictionary = {}
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185
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186 # TODO: Optimize this double loop
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187 for input_false_path in array_inputs:
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188 for key, data_value in inputs_data.items():
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189 if key == input_false_path:
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190 extensionObject = ExtensionClass(input_false_path, data_value)
1
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191 datatype_dictionary.update({data_value["order_index"]: extensionObject})
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192 return datatype_dictionary
1
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193
13
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194 def configure_logger(extra_files_path=None, debug=False):
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195 if not extra_files_path:
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196 raise Exception("Extra files path is not set. Stopping the application")
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197
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198
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199 # All case log: log everything in a .log file
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200 logger_file_name = ''.join([__name__, '.log'])
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201 logging_file_path = os.path.join(extra_files_path, logger_file_name)
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202
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203 logging.basicConfig(filename=logging_file_path, level=logging.DEBUG)
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204
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205 log_stdout = logging.StreamHandler(sys.stdout)
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206 if not debug:
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207 configure_logger_user(log_stdout)
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208 else:
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209 configure_logger_dev(log_stdout)
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210
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211 # stderr configuration
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212 configure_logger_stderr()
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213
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214 logging.debug('#### Welcome in HubArchiveCreator Debug Mode ####\n')
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215
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216 def configure_logger_user(log_stdout=None):
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217 """
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218 User Logger is defined as following:
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219 - User needs to have WARN, ERROR and CRITICAL but well formatted / without traceback
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220 in STDOUT
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221 - Still access to full, brute and traceback for errors
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222 in STDERR
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223 - And further access to debug if needed
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224 in .log
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225 :return:
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226 """
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227 if not log_stdout:
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228 raise Exception("No log_stdout given. Stopping the application")
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229
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230 # stdout for INFO / WARN / ERROR / CRITICAL
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231 log_stdout.setLevel(logging.INFO)
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232
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233 formatter = TraceBackFormatter('%(message)s')
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234
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235 log_stdout.setFormatter(formatter)
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236
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237 logging.getLogger().addHandler(log_stdout)
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238
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239 def configure_logger_dev(log_stdout=None):
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240 """
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241 Dev Logger is defined as following:
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242 - Dev needs to have WARN, ERROR and CRITICAL but well formatted / without traceback, in stdout
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243 - Still access to full, brute and traceback in stderr for errors
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244 - And further access to debug if needed
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245 :return:
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246 """
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247 if not log_stdout:
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248 raise Exception("No log_stdout given. Stopping the application")
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249 log_format = '%(message)s'
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250
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251 # stdout and stderr and both identical for INFO / WARN / ERROR / CRITICAL
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252 log_stdout.setLevel(logging.DEBUG)
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253
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254 formatter = logging.Formatter(log_format)
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255
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256 log_stdout.setFormatter(formatter)
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257
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258 logging.getLogger().addHandler(log_stdout)
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259
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260 def configure_logger_stderr():
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261 """
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262 Configure what should be logged in stderr
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263 :return:
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264 """
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265 log_error = logging.StreamHandler(sys.stderr)
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266 log_error.setLevel(logging.ERROR)
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267 log_error_format = '%(message)s'
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268
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269 formatter_error = logging.Formatter(log_error_format)
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270
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271 log_error.setFormatter(formatter_error)
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272
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273 logging.getLogger().addHandler(log_error)
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274
1
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275 if __name__ == "__main__":
13
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276 logging.getLogger(__name__)
1
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277 main(sys.argv)