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annotate Gff3.py @ 1:fb5e60d4d18a draft

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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
author rmarenco
date Wed, 13 Jul 2016 13:36:37 -0400
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children acc233161f50
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fb5e60d4d18a planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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1 #!/usr/bin/python
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3 import os
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4 import tempfile
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6 # Internal dependencies
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7 from Datatype import Datatype
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8 from Track import Track
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9 from TrackDb import TrackDb
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10 from util import subtools
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13 class Gff3( Datatype ):
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14 def __init__( self, input_Gff3_false_path, data_gff3,
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15 input_fasta_false_path, extra_files_path, tool_directory ):
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16 super( Gff3, self ).__init__(
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17 input_fasta_false_path, extra_files_path, tool_directory
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18 )
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20 self.track = None
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22 self.input_Gff3_false_path = input_Gff3_false_path
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23 self.name_gff3 = data_gff3["name"]
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24 self.priority = data_gff3["order_index"]
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26 # TODO: See if we need these temporary files as part of the generated files
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27 genePredFile = tempfile.NamedTemporaryFile(bufsize=0, suffix=".genePred")
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28 unsortedBedFile = tempfile.NamedTemporaryFile(bufsize=0, suffix=".unsortedBed")
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29 sortedBedFile = tempfile.NamedTemporaryFile(suffix=".sortedBed")
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31 # TODO: Refactor into another Class to manage the twoBitInfo and ChromSizes (same process as in Gtf.py)
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32 twoBitInfoFile = tempfile.NamedTemporaryFile(bufsize=0)
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33 chromSizesFile = tempfile.NamedTemporaryFile(bufsize=0, suffix=".chrom.sizes")
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35 # gff3ToGenePred processing
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36 subtools.gff3ToGenePred(self.input_Gff3_false_path, genePredFile.name)
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38 # TODO: From there, refactor because common use with Gtf.py
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39 # genePredToBed processing
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40 subtools.genePredToBed(genePredFile.name, unsortedBedFile.name)
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42 # Sort processing
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43 subtools.sort(unsortedBedFile.name, sortedBedFile.name)
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45 # Generate the twoBitInfo
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46 subtools.twoBitInfo(self.twoBitFile.name, twoBitInfoFile.name)
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48 # Then we get the output to generate the chromSizes
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49 # TODO: Check if no errors
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50 subtools.sortChromSizes(twoBitInfoFile.name, chromSizesFile.name)
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52 # bedToBigBed processing
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53 # TODO: Change the name of the bb, to tool + genome + possible adding if multiple + .bb
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54 trackName = "".join( (self.name_gff3, ".bb" ) )
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55 myBigBedFilePath = os.path.join(self.myTrackFolderPath, trackName)
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56 with open(myBigBedFilePath, 'w') as bigBedFile:
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57 subtools.bedToBigBed(sortedBedFile.name, chromSizesFile.name, bigBedFile.name)
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59 # Create the Track Object
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60 dataURL = "tracks/%s" % trackName
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62 trackDb = TrackDb(
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63 trackName=trackName,
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64 longLabel=self.name_gff3,
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65 shortLabel=self.getShortName( self.name_gff3 ),
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66 trackDataURL=dataURL,
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67 trackType='bigBed 12 +',
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68 visibility='dense',
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69 priority=self.priority,
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70 )
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72 self.track = Track(
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73 trackFile=myBigBedFilePath,
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74 trackDb=trackDb,
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75 )
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76
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77 print("- %s created in %s" % (trackName, myBigBedFilePath))