Mercurial > repos > rmarenco > hubarchivecreator
annotate util/subtools.py @ 32:6d1f2bb5c1d1 draft default tip
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 5a0c39ed61b4408ba65a759aede807df260d097d
author | yating-l |
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date | Sun, 26 Aug 2018 16:44:27 -0400 |
parents | 7e8a8b732db3 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
rmarenco
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1 #!/usr/bin/python |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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2 # -*- coding: utf8 -*- |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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3 |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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4 """ |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
rmarenco
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5 This class handles the subprocess calls of the different tools used |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
rmarenco
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6 in HubArchiveCreator |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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7 """ |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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8 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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9 import logging |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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10 import os |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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11 import subprocess |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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12 import sys |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 6ac76e7da539ca1773fb809054679f0bf8a06972-dirty
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13 import string |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 93e2e2fb59f99677425104a80c17f665fa7b2b4a-dirty
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14 import tempfile |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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15 |
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16 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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17 class PopenError(Exception): |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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18 def __init__(self, cmd, error, return_code): |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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19 self.cmd = cmd |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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20 self.error = error |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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21 self.return_code = return_code |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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22 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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23 def __str__(self): |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
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24 message = "The subprocess {0} has returned the error: {1}.".format( |
7e8a8b732db3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
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25 self.cmd, self.return_code) |
7e8a8b732db3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
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26 message = ','.join( |
7e8a8b732db3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
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27 (message, "Its error message is: {0}".format(self.error))) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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28 return repr(message) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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29 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
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30 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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31 def _handleExceptionAndCheckCall(array_call, **kwargs): |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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32 """ |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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33 This class handle exceptions and call the tool. |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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34 It maps the signature of subprocess.check_call: |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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35 See https://docs.python.org/2/library/subprocess.html#subprocess.check_call |
fb5e60d4d18a
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
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36 """ |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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37 stdout = kwargs.get('stdout', subprocess.PIPE) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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38 stderr = kwargs.get('stderr', subprocess.PIPE) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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39 shell = kwargs.get('shell', False) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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40 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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41 cmd = array_call[0] |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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42 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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43 output = None |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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44 error = None |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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45 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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46 # TODO: Check the value of array_call and <=[0] |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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47 logging.debug("Calling {0}:".format(cmd)) |
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48 logging.debug("%s", array_call) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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49 logging.debug("---------") |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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50 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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51 # TODO: Use universal_newlines option from Popen? |
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52 try: |
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53 p = subprocess.Popen(array_call, stdout=stdout, |
7e8a8b732db3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
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54 stderr=stderr, shell=shell) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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55 |
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56 # TODO: Change this because of possible memory issues => https://docs.python.org/2/library/subprocess.html#subprocess.Popen.communicate |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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57 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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58 output, error = p.communicate() |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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59 |
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60 if stdout == subprocess.PIPE: |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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61 logging.debug("\t{0}".format(output)) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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62 else: |
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63 logging.debug("\tOutput in file {0}".format(stdout.name)) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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64 # If we detect an error from the subprocess, then we raise an exception |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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65 # TODO: Manage if we raise an exception for everything, or use CRITICAL etc... but not stop process |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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66 # TODO: The responsability of returning a sys.exit() should not be there, but up in the app. |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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67 if p.returncode: |
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68 if stderr == subprocess.PIPE: |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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69 raise PopenError(cmd, error, p.returncode) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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70 else: |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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71 # TODO: To Handle properly with a design behind, if we received a option as a file for the error |
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72 raise Exception("Error when calling {0}. Error as been logged in your file {1}. Error code: {2}" |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit fc73ec22a0db3ab09c4ac13dc58f0b54ae37845c
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73 .format(cmd, stderr.name, p.returncode)) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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74 |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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75 except OSError as e: |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
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76 message = "The subprocess {0} has encountered an OSError: {1}".format( |
7e8a8b732db3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
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77 cmd, e.strerror) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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78 if e.filename: |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
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79 message = '\n'.join( |
7e8a8b732db3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 1a81ebd0ddea950b84af3fc830e9267a4814b29f
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80 (message, ", against this file: {0}".format(e.filename))) |
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planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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81 logging.error(message) |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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82 sys.exit(-1) |
25809f699cb3
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 65ab931ef2b05a5acf06cbde3a746c94a0a0a4cb
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83 except PopenError as p: |
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84 message = "The subprocess {0} has returned the error: {1}.".format( |
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85 p.cmd, p.return_code) |
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86 message = '\n'.join( |
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87 (message, "Its error message is: {0}".format(p.error))) |
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88 |
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89 logging.exception(message) |
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90 |
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91 sys.exit(p.return_code) |
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92 except Exception as e: |
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93 message = "The subprocess {0} has encountered an unknown error: {1}".format( |
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94 cmd, e) |
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95 logging.exception(message) |
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96 |
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97 sys.exit(-1) |
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98 return p |
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99 |
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100 |
1
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101 def twoBitInfo(two_bit_file_name, two_bit_info_file): |
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102 """ |
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103 Call twoBitInfo and write the result into twoBit_info_file |
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104 :param two_bit_file_name: |
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105 :param two_bit_info_file: |
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106 :return the subprocess.check_call return object: |
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107 """ |
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108 array_call = ['twoBitInfo', two_bit_file_name, two_bit_info_file] |
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109 p = _handleExceptionAndCheckCall(array_call) |
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110 return p |
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111 |
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112 |
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113 def faToTwoBit(fasta_file_name, twoBitFile): |
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114 """ |
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115 This function call faToTwoBit UCSC tool, and return the twoBitFile |
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116 :param fasta_file_name: |
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117 :param mySpecieFolder: |
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118 :return: |
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119 """ |
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120 |
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121 array_call = ['faToTwoBit', fasta_file_name, twoBitFile] |
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122 _handleExceptionAndCheckCall(array_call) |
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123 |
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124 return twoBitFile |
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125 |
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126 |
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127 def gtfToGenePred(input_gtf_file_name, gene_pred_file_name): |
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128 """ |
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129 Call gtfToGenePred and write the result into gene_pred_file_name |
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130 :param input_gtf_file_name: |
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131 :param gene_pred_file_name: |
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132 :return: |
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133 """ |
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134 array_call = ['gtfToGenePred', input_gtf_file_name, gene_pred_file_name] |
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135 p = _handleExceptionAndCheckCall(array_call) |
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136 return p |
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137 |
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138 |
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139 def gff3ToGenePred(input_gff3_file_name, gene_pred_file_name): |
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140 """ |
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141 Call gff3ToGenePred and write the result into gene_pred_file_name |
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142 :param input_gff3_file_name: |
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143 :param gene_pred_file_name: |
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144 :return: |
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145 """ |
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146 array_call = ['gff3ToGenePred', input_gff3_file_name, gene_pred_file_name] |
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147 p = _handleExceptionAndCheckCall(array_call) |
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148 return p |
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149 |
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150 |
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151 def genePredToBigGenePred(gene_pred_file_name, unsorted_bigGenePred_file_name): |
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152 """ |
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153 Call genePredToBigGenePred and write the result into unsorted_bigGenePred_file_name |
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154 :param gene_pred_file_name: |
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155 :param unsorted_bigGenePred_file_name: |
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156 :return: |
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157 """ |
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158 array_call = ['genePredToBigGenePred', |
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159 gene_pred_file_name, |
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160 unsorted_bigGenePred_file_name] |
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161 p = _handleExceptionAndCheckCall(array_call) |
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162 return p |
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163 |
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164 |
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165 def genePredToBed(gene_pred_file_name, unsorted_bed_file_name): |
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166 """ |
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167 Call genePredToBed and write the result into unsorted_bed_file_name |
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168 :param gene_pred_file_name: |
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169 :param unsorted_bed_file_name: |
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170 :return: |
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171 """ |
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172 array_call = ['genePredToBed', gene_pred_file_name, unsorted_bed_file_name] |
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173 p = _handleExceptionAndCheckCall(array_call) |
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174 return p |
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175 |
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176 |
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177 def sort(unsorted_bed_file_name, sorted_bed_file_name): |
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178 """ |
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179 Call sort with -k1,1 -k2,2n on unsorted_bed_file_name and write the result into sorted_bed_file_name |
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180 :param unsorted_bed_file_name: |
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181 :param sorted_bed_file_name: |
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182 :return: |
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183 """ |
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184 array_call = ['sort', '-k', '1,1', '-k', '2,2n', |
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185 unsorted_bed_file_name, '-o', sorted_bed_file_name] |
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186 p = _handleExceptionAndCheckCall(array_call) |
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187 return p |
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188 |
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189 |
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190 def sortChromSizes(two_bit_info_file_name, chrom_sizes_file_name): |
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191 """ |
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192 Call sort with -k2rn on two_bit_info_file_name and write the result into chrom_sizes_file_name |
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193 :param two_bit_info_file_name: |
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194 :param chrom_sizes_file_name: |
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195 :return: |
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196 """ |
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197 array_call = ['sort', '-k2rn', two_bit_info_file_name, |
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198 '-o', chrom_sizes_file_name] |
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199 p = _handleExceptionAndCheckCall(array_call) |
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200 return p |
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201 |
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202 |
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203 def bedToBigBed(sorted_bed_file_name, chrom_sizes_file_name, big_bed_file_name, options=None): |
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204 """ |
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205 Call bedToBigBed on sorted_bed_file_name, using chrom_sizes_file_name and write the result into big_bed_file_name |
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206 :param sorted_bed_file_name: |
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207 :param chrom_sizes_file_name: |
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208 :param big_bed_file_name: |
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209 :return: |
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210 """ |
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211 |
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212 array_call = ['bedToBigBed', sorted_bed_file_name, |
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213 chrom_sizes_file_name, big_bed_file_name] |
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214 if options: |
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215 typeOption = options.get("typeOption") |
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216 autoSql = options.get("autoSql") |
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217 tab = options.get("tab") |
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218 extraIndex = options.get("extraIndex") |
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219 if typeOption: |
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220 typeOption = ''.join(['-type=', typeOption]) |
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221 array_call.append(typeOption) |
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222 if autoSql: |
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223 autoSql = ''.join(['-as=', autoSql]) |
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224 array_call.append(autoSql) |
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225 if tab: |
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226 array_call.append('-tab') |
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227 if extraIndex: |
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228 index = ''.join(['-extraIndex=', extraIndex]) |
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229 array_call.append(index) |
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230 p = _handleExceptionAndCheckCall(array_call) |
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231 return p |
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232 |
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233 def sortBam(input_bam_file_name, output_sorted_bam_name): |
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234 """ |
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235 Call samtools on input_bam_file_name and output the result in output_sorted_bam_name |
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236 :param input_bam_file_name: |
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237 :param output_sorted_bam_name: |
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238 :return: |
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239 """ |
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240 array_call = ['samtools', 'sort', |
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241 input_bam_file_name, '-o', output_sorted_bam_name] |
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242 p = _handleExceptionAndCheckCall(array_call) |
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243 return p |
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244 |
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245 |
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246 def createBamIndex(input_sorted_bam_file_name, output_name_index_name): |
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247 """ |
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248 Call `samtools index` on imput_sorted_bam_file_name and output the result in output_name_index_name |
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249 :param input_sorted_bam_file_name: |
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250 :param output_name_index_name: |
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251 :return: |
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252 """ |
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253 array_call = ['samtools', 'index', |
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254 input_sorted_bam_file_name, output_name_index_name] |
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255 p = _handleExceptionAndCheckCall(array_call) |
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256 return p |
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257 |
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258 |
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259 def pslToBigPsl(input_psl_file_name, output_bed12_file_name): |
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260 """ |
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261 Call `pslToBigPsl` on input_psl_file_name and output the result in output_bed12_file_name |
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262 :param input_psl_file_name: Name of the psl input file |
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263 :param output_bed12_file_name: Name of the output file where to store the result of the cmd |
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264 :return: |
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265 """ |
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266 # The command to send |
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267 array_call = ['pslToBigPsl', input_psl_file_name, output_bed12_file_name] |
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268 |
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269 p = _handleExceptionAndCheckCall(array_call) |
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270 return p |
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271 |
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272 # santitize trackName. Because track name must begin with a letter and |
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273 # contain only the following chars: [a-zA-Z0-9_]. |
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274 # See the "track" Common settings at: |
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275 # https://genome.ucsc.edu/goldenpath/help/trackDb/trackDbHub.html#bigPsl_-_Pairwise_Alignments |
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276 |
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277 def validateFiles(input_file, chrom_sizes_file_name, file_type, options=None): |
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278 """ |
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279 Call validateFiles on input_file, using chrom_sizes_file_name and file_type |
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280 :param input_file: |
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281 :param chrom_sizes_file_name: |
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282 :param file_type: |
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283 :return: |
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284 """ |
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285 |
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286 array_call = ['validateFiles', '-chromInfo=' + chrom_sizes_file_name, '-type='+ file_type, input_file] |
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287 if options: |
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288 tab = options.get("tab") |
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289 autoSql = options.get("autoSql") |
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290 logging.debug("tab: {0}".format(tab)) |
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291 logging.debug("autoSql: {0}".format(autoSql)) |
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292 if autoSql: |
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293 autoSql = ''.join(['-as=', autoSql]) |
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294 array_call.append(autoSql) |
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295 if tab: |
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296 array_call.append('-tab') |
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297 p = _handleExceptionAndCheckCall(array_call) |
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298 return p |
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299 |
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300 def pslCheck(input_file, options=None): |
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301 """ |
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302 Call pslCheck on input_file |
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303 :param input_file: |
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304 :return: |
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305 """ |
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306 |
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307 array_call = ['pslCheck', input_file] |
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308 p = _handleExceptionAndCheckCall(array_call) |
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309 return p |
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310 |
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311 |
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312 |