view Gff3.py @ 12:747475757cb0 draft

planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 85389def8ed35b3dc08701963dcae4afd1b5b50a
author rmarenco
date Wed, 27 Jul 2016 10:43:58 -0400
parents d05236b15f81
children 25809f699cb3
line wrap: on
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#!/usr/bin/python

import os
import tempfile

# Internal dependencies
from Datatype import Datatype
from Track import Track
from TrackDb import TrackDb
from util import subtools


class Gff3( Datatype ):
    def __init__(self, input_Gff3_false_path, data_gff3):
        super( Gff3, self ).__init__()

        self.track = None

        self.input_Gff3_false_path = input_Gff3_false_path
        self.name_gff3 = data_gff3["name"]
        self.priority = data_gff3["order_index"]

        # TODO: See if we need these temporary files as part of the generated files
        genePredFile = tempfile.NamedTemporaryFile(bufsize=0, suffix=".genePred")
        unsortedBedFile = tempfile.NamedTemporaryFile(bufsize=0, suffix=".unsortedBed")
        sortedBedFile = tempfile.NamedTemporaryFile(suffix=".sortedBed")

        # TODO: Refactor into another Class to manage the twoBitInfo and ChromSizes (same process as in Gtf.py)

        # gff3ToGenePred processing
        subtools.gff3ToGenePred(self.input_Gff3_false_path, genePredFile.name)

        # TODO: From there, refactor because common use with Gtf.py
        # genePredToBed processing
        subtools.genePredToBed(genePredFile.name, unsortedBedFile.name)

        # Sort processing
        subtools.sort(unsortedBedFile.name, sortedBedFile.name)

        # TODO: Check if no errors

        # bedToBigBed processing
        # TODO: Change the name of the bb, to tool + genome + possible adding if multiple +  .bb
        trackName = "".join( (self.name_gff3, ".bb" ) )
        myBigBedFilePath = os.path.join(self.myTrackFolderPath, trackName)
        with open(myBigBedFilePath, 'w') as bigBedFile:
            subtools.bedToBigBed(sortedBedFile.name, self.chromSizesFile.name, bigBedFile.name)

        # Create the Track Object
        self.createTrack(file_path=trackName,
                         track_name=trackName,
                         long_label=self.name_gff3, track_type='bigBed 12 +', visibility='dense', priority=self.priority,
                         track_file=myBigBedFilePath)

        # dataURL = "tracks/%s" % trackName
        #
        # trackDb = TrackDb(
        #     trackName=trackName,
        #     longLabel=self.name_gff3,
        #     shortLabel=self.getShortName( self.name_gff3 ),
        #     trackDataURL=dataURL,
        #     trackType='bigBed 12 +',
        #     visibility='dense',
        #     priority=self.priority,
        # )
        #
        # self.track = Track(
        #     trackFile=myBigBedFilePath,
        #     trackDb=trackDb,
        # )

        print("- Gff3 %s created" % self.name_gff3)
        #print("- %s created in %s" % (trackName, myBigBedFilePath))