Mercurial > repos > rmarenco > hubarchivecreator
view Gtf.py @ 2:df9e12da0d13 draft
planemo upload for repository https://github.com/goeckslab/hub-archive-creator commit 64cfc08088d11f6818c1b4e5514ef9e67969eaff-dirty
author | rmarenco |
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date | Thu, 14 Jul 2016 12:49:51 -0400 |
parents | fb5e60d4d18a |
children | acc233161f50 |
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#!/usr/bin/python import os import tempfile # Internal dependencies from Datatype import Datatype from Track import Track from TrackDb import TrackDb from util import subtools class Gtf( Datatype ): def __init__( self, input_gtf_false_path, data_gtf, input_fasta_file, extra_files_path, tool_directory ): super(Gtf, self).__init__( input_fasta_file=input_fasta_file, extra_files_path=extra_files_path, tool_directory=tool_directory ) self.track = None self.input_gtf_false_path = input_gtf_false_path self.name_gtf = data_gtf["name"] self.priority = data_gtf["order_index"] print "Creating TrackHub GTF from (falsePath: %s; name: %s)" % ( self.input_gtf_false_path, self.name_gtf) # TODO: See if we need these temporary files as part of the generated files genePredFile = tempfile.NamedTemporaryFile(bufsize=0, suffix=".genePred") unsortedBedFile = tempfile.NamedTemporaryFile(bufsize=0, suffix=".unsortedBed") sortedBedFile = tempfile.NamedTemporaryFile(suffix=".sortedBed") twoBitInfoFile = tempfile.NamedTemporaryFile(bufsize=0) chromSizesFile = tempfile.NamedTemporaryFile(bufsize=0, suffix=".chrom.sizes") # GtfToGenePred subtools.gtfToGenePred(self.input_gtf_false_path, genePredFile.name) # TODO: From there, refactor because common use with Gff3.py # genePredToBed processing subtools.genePredToBed(genePredFile.name, unsortedBedFile.name) # Sort processing subtools.sort(unsortedBedFile.name, sortedBedFile.name) # TODO: Chehck if the twoBitInfo / ChromSizes is redundant and make an intermediate class # Generate the twoBitInfo subtools.twoBitInfo(self.twoBitFile.name, twoBitInfoFile.name) # Then we get the output to generate the chromSizes # TODO: Check if no errors subtools.sortChromSizes(twoBitInfoFile.name, chromSizesFile.name) # bedToBigBed processing # TODO: Change the name of the bb, to tool + genome + possible adding if multiple + .bb trackName = "".join( ( self.name_gtf, ".bb") ) myBigBedFilePath = os.path.join(self.myTrackFolderPath, trackName) with open(myBigBedFilePath, 'w') as bigBedFile: subtools.bedToBigBed(sortedBedFile.name, chromSizesFile.name, bigBedFile.name) # Create the Track Object dataURL = "tracks/%s" % trackName trackDb = TrackDb( trackName=trackName, longLabel=self.name_gtf, shortLabel=self.getShortName( self.name_gtf ), trackDataURL=dataURL, trackType='bigBed 12 +', visibility='dense', priority=self.priority, ) self.track = Track( trackFile=myBigBedFilePath, trackDb=trackDb, ) print("- %s created in %s" % (trackName, myBigBedFilePath))