# HG changeset patch # User rnateam # Date 1527675147 14400 # Node ID cb133602cd9b9410355b00905c2d65a2cf60a504 # Parent 95f779f4adb7ef9306912780ca72153f4d3bea2f planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/chipseeker commit bae8cc7d7a88154b815acc3cd126010e7e965d5c diff -r 95f779f4adb7 -r cb133602cd9b chipseeker.xml --- a/chipseeker.xml Tue May 29 15:08:04 2018 -0400 +++ b/chipseeker.xml Wed May 30 06:12:27 2018 -0400 @@ -23,18 +23,18 @@ -i '$peaks' -G '$gtf' - -u $upstream - -d $downstream - #if $flankgeneinfo: - -F $flankgeneinfo - -D $flankgenedist + -u $adv.upstream + -d $adv.downstream + #if $adv.flankgeneinfo: + -F $adv.flankgeneinfo + -D $adv.flankgenedist #end if -f $format -p $pdf ]]> - + @@ -52,16 +52,19 @@ - - - - + +
+ + + + +
@@ -137,14 +140,23 @@ **What it does** -ChIPseeker_ is a Bioconductor package for annotating ChIP-seq data analysis. See -the `ChIPseeker vignette`_ for more information. +ChIPseeker_ is a Bioconductor package for annotating ChIP-seq data analysis. Peak Annotation is performed by the annotatePeak function. The position and strand information of nearest genes are reported, in addition to the distance from the peak to the TSS of its nearest gene. Users can define the TSS (transcription start site) region under **Advanced Options**, by default the TSS region is defined from -3kb to +3kb. The genomic region of the peak is reported in the annotation column. Since some annotations may overlap for a peak, ChIPseeker adopts the following priority in genomic annotation: + +* Promoter +* 5’ UTR +* 3’ UTR +* Exon +* Intron +* Downstream +* Intergenic + +ChIPseeker also produces plots to help users visualise the overlaps in annotation for peaks, for example, the vennpie and upsetplot. See the `ChIPseeker vignette`_ for more information. ----- **Inputs** -A peaks file in BED or Interval format e.g from MACS2 or DiffBind. +A peaks file in BED, Interval or Tabular format e.g from MACS2 or DiffBind. Example: @@ -167,7 +179,7 @@ This tool outputs * a file of annotated peaks in Interval or Tabular format - * a PDF of plots + * a PDF of plots (plotAnnoPie, vennpie, upsetplot) * the R script used by this tool **Annotated peaks**