Mercurial > repos > rnateam > segemehl
diff segemehl.xml @ 1:df7c7d732d31 draft
Uploaded
author | rnateam |
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date | Wed, 04 Feb 2015 06:52:06 -0500 |
parents | e97db054a88d |
children | 0da425524259 |
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--- a/segemehl.xml Sat Feb 22 06:01:16 2014 -0500 +++ b/segemehl.xml Wed Feb 04 06:52:06 2015 -0500 @@ -4,18 +4,19 @@ <requirement type="package" version="0.1.6">segemehl</requirement> </requirements> <command> +<![CDATA[ ## prepare segemehl index if no reference genome is supplied temp_index = `mktemp`; #if $refGenomeSource.genomeSource == "history": segemehl.x -x $temp_index -d $refGenomeSource.own_reference_genome; #else: - $temp_index = ${refGenomeSource.index.fields.index_path} + #set $temp_index = ${refGenomeSource.index.fields.index_path} #end if ## execute segemehl segemehl.x - + ## number of threads -t "\${GALAXY_SLOTS:-12}" @@ -34,7 +35,7 @@ -q "#echo ' '.join( $query_list )#" #else ## prepare inputs - + #set $mate1 = list() #set $mate2 = list() #for $mate_pair in $library.mate_list: @@ -62,11 +63,12 @@ $order -s -o $segemehl_out +]]> </command> <stdio> - <regex match="Exit forced" - source="both" - level="fatal" + <regex match="Exit forced" + source="both" + level="fatal" description="Execution halted." /> </stdio> <inputs> @@ -137,24 +139,25 @@ <data format="sam" name="segemehl_out" label="Read alignments on ${on_string}"/> </outputs> <help> +<![CDATA[ .. class:: infomark -**What it does** +**What it does** Segemehl_ is a short read mapper with gaps. -Segemehl_ is a software to map short sequencer reads to reference genomes. -Unlike other methods, segemehl is able to detect not only mismatches but also insertions and deletions. -Furthermore, segemehl is not limited to a specific read length and is able to mapprimer- or polyadenylation contaminated reads correctly. +Segemehl_ is a software to map short sequencer reads to reference genomes. +Unlike other methods, segemehl is able to detect not only mismatches but also insertions and deletions. +Furthermore, segemehl is not limited to a specific read length and is able to mapprimer- or polyadenylation contaminated reads correctly. segemehl implements a matching strategy based on enhanced suffix arrays (ESA). Segemehl_ allows bisulfite sequencing mapping and split read mapping. .. _Segemehl: http://www.bioinf.uni-leipzig.de/Software/segemehl/ -**References** -Hoffmann S, Otto C, Kurtz S, Sharma CM, Khaitovich P, Vogel J, Stadler PF, Hackermueller J: "Fast mapping of short sequences with mismatches, insertions and deletions using index structures", PLoS Comput Biol (2009) vol. 5 (9) pp. e1000502 -download latest version: 0.1.6 manual: download here new stuff: faster multiple split read mapping bug fixes: bugfixes: increased sensitivity for strand switches changes: - default accuracy now 90% older segemehl indices are still usable. issues: untraceable errors with gcc compiler gcc-4.5. zlib linker problems with some ubuntu versions complaint department: steve bioinf uni leipzig deshapeimage_1_link_0shapeimage_1_link_1 - +]]> </help> + <citations> + <citation type="doi">10.1371/journal.pcbi.1000502</citation> + </citations> </tool>