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1 #include <string.h>
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2 #include <unistd.h>
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3 #include "faidx.h"
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4 #include "sam.h"
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5
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6 #define TYPE_BAM 1
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7 #define TYPE_READ 2
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8
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9 bam_header_t *bam_header_dup(const bam_header_t *h0)
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10 {
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11 bam_header_t *h;
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12 int i;
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13 h = bam_header_init();
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14 *h = *h0;
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15 h->hash = 0;
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16 h->text = (char*)calloc(h->l_text + 1, 1);
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17 memcpy(h->text, h0->text, h->l_text);
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18 h->target_len = (uint32_t*)calloc(h->n_targets, 4);
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19 h->target_name = (char**)calloc(h->n_targets, sizeof(void*));
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20 for (i = 0; i < h->n_targets; ++i) {
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21 h->target_len[i] = h0->target_len[i];
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22 h->target_name[i] = strdup(h0->target_name[i]);
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23 }
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24 if (h0->rg2lib) h->rg2lib = bam_strmap_dup(h0->rg2lib);
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25 return h;
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26 }
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27 static void append_header_text(bam_header_t *header, char* text, int len)
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28 {
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29 int x = header->l_text + 1;
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30 int y = header->l_text + len + 1; // 1 byte null
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31 if (text == 0) return;
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32 kroundup32(x);
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33 kroundup32(y);
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34 if (x < y) header->text = (char*)realloc(header->text, y);
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35 strncpy(header->text + header->l_text, text, len); // we cannot use strcpy() here.
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36 header->l_text += len;
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37 header->text[header->l_text] = 0;
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38 }
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39
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40 samfile_t *samopen(const char *fn, const char *mode, const void *aux)
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41 {
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42 samfile_t *fp;
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43 fp = (samfile_t*)calloc(1, sizeof(samfile_t));
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44 if (mode[0] == 'r') { // read
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45 fp->type |= TYPE_READ;
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46 if (mode[1] == 'b') { // binary
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47 fp->type |= TYPE_BAM;
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48 fp->x.bam = strcmp(fn, "-")? bam_open(fn, "r") : bam_dopen(fileno(stdin), "r");
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49 if (fp->x.bam == 0) goto open_err_ret;
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50 fp->header = bam_header_read(fp->x.bam);
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51 } else { // text
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52 fp->x.tamr = sam_open(fn);
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53 if (fp->x.tamr == 0) goto open_err_ret;
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54 fp->header = sam_header_read(fp->x.tamr);
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55 if (fp->header->n_targets == 0) { // no @SQ fields
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56 if (aux) { // check if aux is present
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57 bam_header_t *textheader = fp->header;
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58 fp->header = sam_header_read2((const char*)aux);
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59 append_header_text(fp->header, textheader->text, textheader->l_text);
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60 bam_header_destroy(textheader);
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61 }
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62 if (fp->header->n_targets == 0)
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63 fprintf(stderr, "[samopen] no @SQ lines in the header.\n");
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64 } else fprintf(stderr, "[samopen] SAM header is present: %d sequences.\n", fp->header->n_targets);
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65 }
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66 sam_header_parse_rg(fp->header);
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67 } else if (mode[0] == 'w') { // write
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68 fp->header = bam_header_dup((const bam_header_t*)aux);
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69 if (mode[1] == 'b') { // binary
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70 char bmode[3];
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71 bmode[0] = 'w'; bmode[1] = strstr(mode, "u")? 'u' : 0; bmode[2] = 0;
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72 fp->type |= TYPE_BAM;
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73 fp->x.bam = strcmp(fn, "-")? bam_open(fn, bmode) : bam_dopen(fileno(stdout), bmode);
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74 if (fp->x.bam == 0) goto open_err_ret;
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75 bam_header_write(fp->x.bam, fp->header);
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76 } else { // text
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77 // open file
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78 fp->x.tamw = strcmp(fn, "-")? fopen(fn, "w") : stdout;
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79 if (fp->x.tamr == 0) goto open_err_ret;
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80 if (strstr(mode, "X")) fp->type |= BAM_OFSTR<<2;
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81 else if (strstr(mode, "x")) fp->type |= BAM_OFHEX<<2;
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82 else fp->type |= BAM_OFDEC<<2;
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83 // write header
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84 if (strstr(mode, "h")) {
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85 int i;
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86 bam_header_t *alt;
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87 // parse the header text
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88 alt = bam_header_init();
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89 alt->l_text = fp->header->l_text; alt->text = fp->header->text;
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90 sam_header_parse(alt);
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91 alt->l_text = 0; alt->text = 0;
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92 // check if there are @SQ lines in the header
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93 fwrite(fp->header->text, 1, fp->header->l_text, fp->x.tamw);
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94 if (alt->n_targets) { // then write the header text without dumping ->target_{name,len}
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95 if (alt->n_targets != fp->header->n_targets)
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96 fprintf(stderr, "[samopen] inconsistent number of target sequences.\n");
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97 } else { // then dump ->target_{name,len}
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98 for (i = 0; i < fp->header->n_targets; ++i)
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99 fprintf(fp->x.tamw, "@SQ\tSN:%s\tLN:%d\n", fp->header->target_name[i], fp->header->target_len[i]);
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100 }
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101 bam_header_destroy(alt);
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102 }
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103 }
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104 }
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105 return fp;
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106
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107 open_err_ret:
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108 free(fp);
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109 return 0;
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110 }
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111
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112 void samclose(samfile_t *fp)
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113 {
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114 if (fp == 0) return;
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115 if (fp->header) bam_header_destroy(fp->header);
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116 if (fp->type & TYPE_BAM) bam_close(fp->x.bam);
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117 else if (fp->type & TYPE_READ) sam_close(fp->x.tamr);
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118 else fclose(fp->x.tamw);
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119 free(fp);
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120 }
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121
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122 int samread(samfile_t *fp, bam1_t *b)
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123 {
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124 if (fp == 0 || !(fp->type & TYPE_READ)) return -1; // not open for reading
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125 if (fp->type & TYPE_BAM) return bam_read1(fp->x.bam, b);
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126 else return sam_read1(fp->x.tamr, fp->header, b);
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127 }
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128
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129 int samwrite(samfile_t *fp, const bam1_t *b)
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130 {
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131 if (fp == 0 || (fp->type & TYPE_READ)) return -1; // not open for writing
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132 if (fp->type & TYPE_BAM) return bam_write1(fp->x.bam, b);
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133 else {
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134 char *s = bam_format1_core(fp->header, b, fp->type>>2&3);
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135 int l = strlen(s);
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136 fputs(s, fp->x.tamw); fputc('\n', fp->x.tamw);
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137 free(s);
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138 return l + 1;
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139 }
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140 }
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141
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142 int sampileup(samfile_t *fp, int mask, bam_pileup_f func, void *func_data)
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143 {
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144 bam_plbuf_t *buf;
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145 int ret;
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146 bam1_t *b;
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147 b = bam_init1();
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148 buf = bam_plbuf_init(func, func_data);
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149 bam_plbuf_set_mask(buf, mask);
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150 while ((ret = samread(fp, b)) >= 0)
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151 bam_plbuf_push(b, buf);
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152 bam_plbuf_push(0, buf);
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153 bam_plbuf_destroy(buf);
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154 bam_destroy1(b);
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155 return 0;
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156 }
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157
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158 char *samfaipath(const char *fn_ref)
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159 {
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160 char *fn_list = 0;
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161 if (fn_ref == 0) return 0;
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162 fn_list = calloc(strlen(fn_ref) + 5, 1);
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163 strcat(strcpy(fn_list, fn_ref), ".fai");
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164 if (access(fn_list, R_OK) == -1) { // fn_list is unreadable
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165 if (access(fn_ref, R_OK) == -1) {
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166 fprintf(stderr, "[samfaipath] fail to read file %s.\n", fn_ref);
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167 } else {
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168 fprintf(stderr, "[samfaipath] build FASTA index...\n");
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169 if (fai_build(fn_ref) == -1) {
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170 fprintf(stderr, "[samfaipath] fail to build FASTA index.\n");
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171 free(fn_list); fn_list = 0;
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172 }
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173 }
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174 }
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175 return fn_list;
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176 }
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