Mercurial > repos > sarahinraauzeville > tax4fun
changeset 9:bd5ba02b1f71 draft
Uploaded
author | sarahinraauzeville |
---|---|
date | Thu, 21 Dec 2017 09:08:53 -0500 |
parents | a6a0c9a8ad51 |
children | 444f1e7fe177 |
files | sm_Tax4Fun.pl |
diffstat | 1 files changed, 143 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/sm_Tax4Fun.pl Thu Dec 21 09:08:53 2017 -0500 @@ -0,0 +1,143 @@ +#Declaration de l espace de travail et du chemin de l executable R : +use strict; +use Carp; +use Statistics::R; +use Cwd qw(abs_path); +use File::Basename; +use Getopt::Long; + + +#Variables Globales ########################### +defined $ENV{'MY_GALAXY_DIR'} || die "MY_GALAXY_DIR environment variable not defined"; +use lib "$ENV{'MY_GALAXY_DIR'}"; +use GalaxyPath; + +my ($r_bin,$log_dir,$R,$cmd,$line, $out, $input, $reference); + + +#test: +#perl sm_Tax4Fun.pl --input DATA/testtax4fun---ssu---fingerprint----Total---sim_93---tax_silva---td_20.csv --out DATA/Tax4FunProfile.txt + + +#Parametres d'entree +Getopt::Long::Configure( 'no_ignorecase', 'bundling' ); +GetOptions ( + 'out=s' => \$out, + 'input=s' => \$input, + 'reference=s' => \$reference +) or die "Usage: Error in command line arguments\n"; + +# SETTING GLOBAL VARIABLES #################################################### +my $cfg = GalaxyPath->new( -file => $ENV{"GALAXY_CONFIG_FILE"} ); +my $file_path = $cfg->my_path( 'workPath', 'MYWORKSPACE' ); +$r_bin = $cfg->my_path( 'toolsPath', 'R_BIN_PATH' ); +my $SCRIPTS_R_path = $cfg->my_path( 'toolsPath', 'SCRIPTS_R_path' ); +############################################################################### + +#workspace +my $debug = 0; #Mode debug (0) +if ($debug == 0) + { + print STDOUT "Debug mode OK \n"; + } +else + { + $file_path = dirname($out); + print STDOUT "No debug \n"; + } + + + +my ($nb) = ($out=~/dataset_(\d+)\.\S+$/); +#For debugging in command line on vm-galaxy-preprod +#my ($nb) = (1); +#creation du repertoire de sortie +`cd $file_path; mkdir $nb; chmod -R 777 $nb `; +my $dirresults= $file_path."/".$nb; + + +print STDOUT "Job working directory : $dirresults \n"; + + + +#remplacer les [a-z]__ du fichier entrant $input par rien +#$str =~ s/$find/$replace/g; +`cp -a $input $dirresults/INPUT.csv`; +open(FILE, "<$dirresults/INPUT.csv") || die "File not found"; +my @lines = <FILE>; +close(FILE); + +my @newlines; +my %Taxo=(); +foreach(@lines) { + $_ =~ s/[a-z]\_\_//g; #homogeneiser la taxonomie issues de frogs avec celle utilisée par tax4fun + $_ =~ s/\n/;\n/g; #pour qu'il y ait un point virgule a la fin de chaque ligne + $_ =~ s/;;/;/g; #remplacer les ;; par un seul ; + $_ =~ s/;;/;/g; #remplacer les ;; par un seul ; une seconde fois pour les triples points virgules + #push(@newlines,$_); + /^(\d+)\t(.+)/||die "bad line: $_"; + $Taxo{$2}+=$1; +} + +open(FILE, ">$dirresults/INPUT.csv") || die "File not found"; +#print FILE "abondance_sum\tTaxonomy\n"; +print FILE "abondance_sum;\n"; +#print FILE @newlines; +foreach my $taxo (sort {$a cmp $b} keys %Taxo) { + printf FILE "%d\t%s\n",$Taxo{$taxo},$taxo; +} +close(FILE); + + +#Log dir +$log_dir = $dirresults; + +#déaration de l'objet $R et ouverture du pont : +$R = Statistics::R->new( + "r_bin" => $r_bin, + "log_dir" => $log_dir, +) or die "Problem with R : $!\n"; +# Ouverture du pont +$R->startR; + + +print STDOUT "Ouverture du pont R \n"; + +#Read R script +$cmd=""; +#[galaxy-preprod@vm-galaxy-preprod sm_Tax4Fun]$ ln -s /galaxydata/galaxy-preprod/my_tools/sm_Tax4Fun/sm_Tax4Fun.R /galaxydata/galaxy-preprod/my_bin/scripts_R/. +open IN,"< $SCRIPTS_R_path/sm_Tax4Fun.R" + or die "Unable to read R script : $!\n"; +while ($line=<IN>) { + $cmd.=$line; +} +close(IN); + +#Declare R function +print STDOUT "Declaration du script R \n"; + +#$dirresults/INPUT.csv #"input.file = '$input', ". +$R->send( + "setwd('$dirresults')\n". + "$cmd\n". + "GalaxyFrogsTax( ". + "input.file = '$dirresults/INPUT.csv', ". + "reference = '$reference', ". + "output.file = '$out')\n"); + +print STDOUT "Envoi du script R "; + + + + +#Fermeture du pont +$R->stopR(); + +print STDOUT "Fermeture du pont R \n"; + +#Recuperation des outputs dans Galaxy +my $cmdOUT =''; +my $outTax= "$dirresults/Tax4FunProfile.txt"; +if (! -e $out){print STDERR "Pas de fichier Tax4FunProfile.txt produit \n";} +else {$cmdOUT = "(mv $outTax $out) >& ./cp_taxout.log 2>&1"; +system $cmdOUT;}