Mercurial > repos > sblanck > smagexp
annotate MetaRNASeq.R @ 4:58052f8bc987 draft
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 8d4651e98155855108d1c4574392d503cc04bc95
author | sblanck |
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date | Thu, 01 Mar 2018 05:22:24 -0500 |
parents | 1024245abc70 |
children | 3ce32282f6a4 |
rev | line source |
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1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
sblanck
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1 #!/usr/bin/env Rscript |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
sblanck
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2 # setup R error handling to go to stderr |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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3 options( show.error.messages=F, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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4 |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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5 # we need that to not crash galaxy with an UTF8 error on German LC settings. |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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6 loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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7 |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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8 library("optparse") |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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9 |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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10 ##### Read options |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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11 option_list=list( |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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12 make_option("--input",type="character",default="NULL",help="list of rdata objects containing eset objects"), |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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13 make_option("--result",type="character",default=NULL,help="text file containing result of the meta-analysis"), |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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14 make_option("--htmloutput",type="character",default=NULL,help="Output html report"), |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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15 make_option("--htmloutputpath",type="character",default="NULL",help="Path of output html report"), |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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16 make_option("--htmltemplate",type="character",default=NULL,help="html template)") |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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17 ); |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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18 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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19 opt_parser = OptionParser(option_list=option_list); |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
sblanck
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20 opt = parse_args(opt_parser); |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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21 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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22 if(is.null(opt$input)){ |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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23 print_help(opt_parser) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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24 stop("input required.", call.=FALSE) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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25 } |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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26 |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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27 #loading libraries |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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28 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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29 suppressPackageStartupMessages(require(affy)) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
sblanck
parents:
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30 suppressPackageStartupMessages(require(annaffy)) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
sblanck
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31 suppressPackageStartupMessages(require(VennDiagram)) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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32 suppressPackageStartupMessages(require(GEOquery)) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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33 |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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34 listInput <- trimws( unlist( strsplit(trimws(opt$input), ",") ) ) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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35 |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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36 listfiles=vector() |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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37 listfilenames=vector() |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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38 |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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39 for (i in 1:length(listInput)) |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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40 { |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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41 inputFileInfo <- unlist( strsplit( listInput[i], ';' ) ) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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42 listfiles=c(listfiles,inputFileInfo[1]) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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43 listfilenames=c(listfilenames,inputFileInfo[2]) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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44 } |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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45 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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46 outputfile <- opt$result |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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47 result.html = opt$htmloutput |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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48 html.files.path=opt$htmloutputpath |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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49 result.template=opt$htmltemplate |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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50 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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51 alpha=0.05 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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52 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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53 #print(comparison) |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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54 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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55 listData=lapply(listfiles,read.table) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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56 orderData=lapply(listData, function(x) x[order(x[1]), ]) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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57 rawpval=lapply(orderData,function(x) x[6]) |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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58 rawpval=lapply(rawpval, function(x) as.numeric(unlist(x))) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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59 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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60 DE=list() |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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61 DE=lapply(orderData, function(x) ifelse(x[7]<=0.05,1,0)) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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62 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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63 FC=list() |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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64 FC=lapply(orderData, function(x) x[3]) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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65 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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66 DE=as.data.frame(DE) |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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67 colnames(DE)=listfilenames |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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68 FC=as.data.frame(FC) |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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69 colnames(FC)=listfilenames |
1024245abc70
planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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70 # the comparison must only have two values and the conds must |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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71 # be a vector from those values, at least one of each. |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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72 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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73 #if (length(comparison) != 2) { |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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74 # stop("Comparison type must be a tuple: ", cargs[length(cargs) - 8]) |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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75 #} |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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76 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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77 sink("/dev/null") |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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78 dir.create(html.files.path, recursive=TRUE) |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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79 #library(DESeq) |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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80 #library(HTSFilter) |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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81 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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82 #DE=list() |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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83 #FC=list() |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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84 #i=1 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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85 |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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86 # Open the html output file |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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87 #file.conn = file(diag.html, open="w") |
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88 |
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89 #writeLines( c("<html><body>"), file.conn) |
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90 |
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91 # Perform deseq analysis on each study |
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92 #for(i in 1:length(listfiles)) |
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93 #{ |
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94 # f=listfiles[i] |
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95 # fname=listfilenames[i] |
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96 # study_name=unlist(strsplit(fname,"[.]"))[1] |
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97 # print(paste0("study.name ",study_name)) |
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98 # d <- read.table(f, sep=" ", header=TRUE, row.names=1) |
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99 # conds<-sapply(strsplit(colnames(d),"[.]"),FUN=function(x) x[1]) |
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100 # if (length(unique(conds)) != 2) { |
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101 # warning(as.data.frame(strsplit(colnames(d),"[.]"))) |
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102 # stop("You can only have two columns types: ", paste(conds,collapse=" ")) |
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103 # } |
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104 # if (!identical(sort(comparison), sort(unique(conds)))) { |
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105 # stop("Column types must use the two names from Comparison type, and vice versa. Must have at least one of each in the Column types.\nColumn types: ", cargs[2], "\n", "Comparison type: ", cargs[3]) |
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106 # } |
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107 # if (length(d) != length(conds)) { |
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108 # stop("Number of total sample columns in counts file must correspond to the columns types field. E.g. if column types is 'kidney,kidney,liver,liver' then number of sample columns in counts file must be 4 as well.") |
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109 # } |
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110 # |
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111 # cds <- newCountDataSet(d, conds) |
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112 # cds <- estimateSizeFactors(cds) |
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113 # |
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114 # cdsBlind <- estimateDispersions( cds, method="blind" ) |
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115 # |
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116 # if (length(conds) != 2) { |
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117 # cds <- estimateDispersions( cds ) |
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118 # norep = FALSE |
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119 # } |
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120 # |
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121 # if (length(conds) == 2) { |
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122 # cds <- estimateDispersions( cds, method=method, sharingMode=mod, fitType="parametric" ) |
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123 # norep = TRUE |
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124 # } |
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125 # |
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126 # filter<-HTSFilter(cds, plot=FALSE) |
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127 # cds.filter<-filter$filteredData |
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128 # on.index<-which(filter$on==1) |
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129 # |
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130 # res<-as.data.frame(matrix(NA,nrow=nrow(cds),ncol=ncol(cds))) |
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131 # nbT <- nbinomTest(cds.filter, comparison[1], comparison[2]) |
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132 # colnames(res)<-colnames(nbT) |
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133 # res[on.index,]<-nbT |
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134 # #write.table(res[order(res$padj), ], file=outputfile, quote=FALSE, row.names=FALSE, sep="\t") |
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135 # |
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136 # |
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137 # temp.pval.plot = file.path( html.files.path, paste("PvalHist",i,".png",sep="")) |
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138 # png( temp.pval.plot, width=500, height=500 ) |
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139 # hist(res$pval, breaks=100, col="skyblue", border="slateblue", main="") |
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140 # dev.off() |
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141 # |
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142 # writeLines( c("<h2>P-value histogram for ",study_name,"</h2>"), file.conn) |
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143 # writeLines( c("<img src='PvalHist",i,".png'><br/><br/>"), file.conn) |
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144 # |
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145 # #on enregistre la p-value |
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146 # rawpval[[study_name]]<-res$pval |
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147 # DE[[study_name]]<-ifelse(res$padj<=alpha,1,0) |
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148 # FC[[study_name]]<-res$log2FoldChange |
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149 # |
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150 # i=i+1 |
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151 #} |
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152 |
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153 |
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154 # combinations |
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155 library(metaRNASeq) |
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156 fishcomb<-fishercomb(rawpval, BHth=alpha) |
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157 warning(length(rawpval)) |
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158 invnormcomb<-invnorm(rawpval, nrep=c(8,8), BHth=alpha) |
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159 #DE[["fishercomb"]]<-ifelse(fishcomb$adjpval<=alpha,1,0) |
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160 #DE[["invnormcomb"]]<-ifelse(invnormcomb$adjpval<=alpha,1,0) |
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161 |
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162 signsFC<-mapply(FC,FUN=function(x) sign(x)) |
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163 sumsigns<-apply(signsFC,1,sum) |
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164 commonsgnFC<-ifelse(abs(sumsigns)==dim(signsFC)[2],sign(sumsigns),0) |
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165 |
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166 DEresults <- data.frame(DE=DE,"DE.fishercomb"=ifelse(fishcomb$adjpval<=alpha,1,0),"DE.invnorm"=ifelse(invnormcomb$adjpval<=alpha,1,0)) |
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167 |
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168 unionDE <- unique(c(fishcomb$DEindices,invnormcomb$DEindices)) |
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169 FC.selecDE <- data.frame(DEresults[unionDE,],FC[unionDE,],signFC=commonsgnFC[unionDE]) |
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170 keepDE <- FC.selecDE[which(abs(FC.selecDE$signFC)==1),] |
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171 |
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172 fishcomb_de <- rownames(keepDE)[which(keepDE[,"DE.fishercomb"]==1)] |
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173 invnorm_de <- rownames(keepDE)[which(keepDE[,"DE.invnorm"]==1)] |
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174 indstudy_de = list() |
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175 for (i in 1:length(listfiles)) { |
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176 currentIndstudy_de = rownames(keepDE)[which(keepDE[,i]==1)] |
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177 indstudy_de[[listfilenames[i]]]=currentIndstudy_de |
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178 } |
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179 |
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180 IDDIRRfishcomb=IDD.IRR(fishcomb_de,indstudy_de) |
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181 IDDIRRinvnorm=IDD.IRR(invnorm_de,indstudy_de) |
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182 |
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183 #conflits<-data.frame(ID=listData[[1]][rownames(DEresults),1],Fishercomb=DEresults[["DE.fishercomb"]],Invnormcomb=DEresults[["DE.invnorm"]],sign=commonsgnFC) |
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planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit 5974f806f344dbcc384b931492d7f023bfbbe03b
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184 conflits<-data.frame(ID=listData[[1]][rownames(DEresults),1],DE=DEresults,FC=FC,signFC=commonsgnFC) |
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185 #write DE outputfile |
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186 write.table(conflits, outputfile,sep="\t",,row.names=FALSE) |
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187 library(VennDiagram) |
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188 DE_num=apply(DEresults, 2, FUN=function(x) which(x==1)) |
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189 venn.plot<-venn.diagram(x=as.list(DE_num),filename=NULL, col="black", fill=1:length(DE_num)+1,alpha=0.6) |
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190 temp.venn.plot = file.path( html.files.path, paste("venn.png")) |
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191 png(temp.venn.plot,width=500,height=500) |
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192 grid.draw(venn.plot) |
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193 dev.off() |
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194 |
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195 library(jsonlite) |
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196 matrixConflits=as.matrix(conflits) |
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197 datajson=toJSON(matrixConflits,pretty = TRUE) |
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198 summaryFishcombjson=toJSON(as.matrix(t(IDDIRRfishcomb)),pretty = TRUE) |
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199 summaryinvnormjson=toJSON(as.matrix(t(IDDIRRinvnorm)),pretty = TRUE) |
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200 |
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201 |
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202 #vennsplit=strsplit(result.venn,split="/")[[1]] |
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203 #venn=paste0("./",vennsplit[length(vennsplit)]) |
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204 |
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205 |
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206 vennFilename="venn.png" |
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207 vennFile=file.path(html.files.path,vennFilename) |
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208 htmlfile=readChar(result.template, file.info(result.template)$size) |
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209 htmlfile=gsub(x=htmlfile,pattern = "###DATAJSON###",replacement = datajson, fixed = TRUE) |
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210 htmlfile=gsub(x=htmlfile,pattern = "###FISHSUMMARYJSON###",replacement = summaryFishcombjson, fixed = TRUE) |
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211 htmlfile=gsub(x=htmlfile,pattern = "###INVSUMMARYJSON###",replacement = summaryinvnormjson, fixed = TRUE) |
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212 htmlfile=gsub(x=htmlfile,pattern = "###VENN###",replacement = vennFilename, fixed = TRUE) |
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213 write(htmlfile,result.html) |
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214 |
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215 #library(VennDiagram) |
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216 #flog.threshold(ERROR) |
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217 # |
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218 ##venn.plot<-venn.diagram(x = c(res[c(1:(length(res)-3))],meta=list(res$Meta)),filename = v, col = "black", fill = c(1:(length(res)-2)), margin=0.05, alpha = 0.6,imagetype = "png") |
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219 #dir.create(result.path, showWarnings = TRUE, recursive = FALSE) |
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220 # |
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221 #showVenn<-function(liste,file) |
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222 #{ |
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223 # venn.plot<-venn.diagram(x = liste, |
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224 # filename = vennFilename, col = "black", |
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225 # fill = 1:length(liste)+1, |
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226 # margin=0.05, alpha = 0.6,imagetype = "png") |
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227 ## png(file); |
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228 ## grid.draw(venn.plot); |
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229 ## dev.off(); |
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230 # |
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231 #} |
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232 # |
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233 #l=list() |
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234 #for(i in 1:length(esets)) |
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235 #{ |
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236 # l[[paste("study",i,sep="")]]<-res[[i]] |
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237 #} |
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238 #l[["Meta"]]=res[[length(res)-1]] |
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239 #showVenn(l,vennFile) |
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240 #file.copy(vennFilename,result.path) |
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241 |
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242 |
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243 #writeLines( c("<h2>Venn Plot</h2>"), file.conn) |
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244 #writeLines( c("<img src='venn.png'><br/><br/>"), file.conn) |
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245 #writeLines( c("</body></html>"), file.conn) |
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246 #close(file.conn) |
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247 #print("passe6") |
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248 #sink(NULL) |